cDNA Clone CDK13

• Plasmid: pET156P CDK13
• Parent Plasmid: pET15b 6His 3C
• Expressed: His-CDK13
• Genbank: Q14004.2
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCatgccgagcagctcggacacggcgctggggggaggcgggggcct
gagctgggcggagaagaggttggaggaacgccgcaagcggaggcgattcc
tgtcccctcagcagccgccgctgctgttgccgctcctgcagccgcagctc
ctgcaaccgccgccgcccccgccgcctctgctcttcctggctgctcccgg
cacggccgccgccgcagccgccgccgccgcggcctcctcctcttgcttca
gcccgggcccccctctggaggtcaagcggctggcgagaggcaagaggcgc
gcaggagggcggcagaagcggcgtcgcgggccccgcgccgggcaggaggc
ggagaagcgtcgggtcttctcgctgccccagccgcagcaggacggcggtg
gcggtgctagtagcggcgggggtgtgaccccgctggtggaatacgaggat
gtgagctcccagtccgagcaggggctgctgctggggggggccagcgcggc
aacggcggcgacggctgccgggggaacggggggcagcggcgggagtccgg
cctcctcctccggcacccagcggcgcggggaggggtcggagcgcaggccc
cgccgggaccgccgcagcagcagtggccgcagcaaagagcgccaccgcga
gcaccggcggcgggatgggcagcgcggtggcagcgaggcctccaagtccc
gcagccgccacagccacagcggcgaggaacgggccgaggtcgccaagagc
ggcagcagcagcagcagcggcggTcgccggaaaagcgcttcggccacatc
cagcagcagtagcagccgcaaggaccgggactcgaaggcccaccgcagcc
ggactaagtcgtccaaggagccgccttcggcctacaaggaaccgcccaag
gcctaccgggaggacaagaccgagcctaaggcctacaggcggcggcggtc
cctcagcccactgggaggccgggacgacagcccggtgtcccacagggcct
ctcagagcctgaggagccgcaagtcccccagcccggcaggaggtggcagc
agcccctattctcggcggctgccgcgctccccgagcccctacagtcgccg
ccgctcccccagctacagccgccacagctcctacgagcggggcggcgacg
tgtcccctagtccctacagcagcagcagctggcgccgctctcgcagtccc
tacagccctgtgctcagacggtctggaaaatcccgaagcagaagcccgta
ttcatctaggcattcaagatctcgtagcaggcacagattgtctagatcca
gaagtcgtcattctagtatttctcctagcacactaactctgaagagtagc
ctggcagctgaattgaacaagaataaaaaagcacgagcagcagaggcagc
aagagccgcagaagcagcgaaagctgcagaagcaactaaggctgctgagg
ctgctgccaaggctgcaaaagcttcaaacacttctacacctaccaagggg
aacacggaaactagtgccagtgcatcacaaacaaaccatgtgaaggatgt
gaagaaaattaaaattgaacatgcaccttctccctcaagtggtggaactt
taaaaaatgacaaagcaaaaacaaagccacctcttcaggtaacgaaggtg
gaaaataatttgattgtagataaagccaccaagaaagcagtcatagttgg
aaaggagagtaaatctgctgctacaaaggaggaatcagtatctcttaaag
agaaaaccaaaccacttacaccaagcataggagccaaggagaaggagcaa
catgtagctttagtcacctctacattaccaccgttacctttgcctcccat
gctgcctgaagataaagaagctgatagcttacgaggaaatatttcagtaa
aagcagttaaaaaagaagtagaaaagaaactccgatgtcttcttgctgat
ttaccgctgccccctgagctaccaggaggagatgatctttcaaagagtcc
agaggaaaagaaaacaacaacacagttacatagtaaaaggaggcctaaaa
tatgtgggcctcgctatggtgaaaccaaagaaaaagatattgactgggga
aaactctgcgtggataaatttgatatcatcggaattattggagaaggtac
ttacggacaagtttacaaagccagggataaagacactggagaaatggtag
ccttaaaaaaagtacgtctggataatgaaaaggaaggctttccaattaca
gcaattcgagaaattaaaattctccggcagcttacccatcagagtattat
caatatgaaggaaatagtgactgataaagaagatgctttggatttcaaga
aggacaaaggtgcattttatctggtgtttgaatatatggaccatgatctg
atgggactactggaatcaggcttggttcattttaatgaaaatcacataaa
gtcatttatgagacagctcatggagggtctggattattgtcataagaaga
actttttgcatagagatattaaatgttccaatatccttctaaataataga
gggcagataaaacttgcagactttggacttgctcgattgtatagctcaga
agaaagtcggccgtatactaacaaggtaattactttatggtaccgtccac
ctgaactgctactgggagaagaacgatacacaccagccattgatgtatgg
agctgtggctgtatccttggcgaactcttcactaaaaaacctatatttca
agcaaatcaggaacttgcacaactagaattaataagccgaatatgtggga
gtccatgtcctgcagtgtggcctgatgtaatcaaactaccatatttcaac
accatgaaaccaaagaagcaatatcgtcgaaagttaagagaagaatttgt
ttttattcctgcagctgcgctagacttatttgattacatgcttgccttgg
aCcctagtaagcgctgcactgctgaacaggctcttcagtgcgagttcctc
cgagatgtggaaccctcaaaaatgcctccaccagatctccctttatggca
agattgtcatgagttatggagtaaaaagcgaagaagacagaagcagatgg
gcatgactgatgatgtttccacaattaaagcccccaggaaggacttgtct
ctgggcttggatgacagcagaaccaacacaccccagggtgtgctgccatc
ttcacagctgaaatctcagggcagctcaaatgtggcacctgtaaaaacag
gccctggacagcacttaaaccacagtgaattggcaattctactaaaccta
ctacaatctaaaacaagtgttaatatggctgattttgtccaagtgttgaa
cattaaggtaaactctgagactcaacagcagctaaataaaataaaccttc
ctgctggaattttggcaacaggtgaaaaacagacagatccatcaacacca
caacaggagtcttcgaaaccgttgggaggaattcagccttcttctcagac
catccagcctaaaatggagactgatgctgcccaggcggctgtgcagagtg
catttgcagttctgttgactcagttaataaaggctcagcagtcaaagcag
aaagatgtgctactagaagagagggaaaatggatcgggacatgaagcgtc
attacaactcaggccacctccagaacctagcactccggtgtcgggacaag
atgacctcatccagcatcaagatatgaggatcttggagctaacgccagaa
ccagaccggcctcgaattctgcctcctgaccaacgacctcccgagcctcc
tgaaccaccaccagtcactgaggaagatctagattatcggacagaaaacc
agcatgtacccaccaccagttcttcattaactgaccctcatgccggagtg
aaggcagccctgttacagctgcttgctcagcatcagccccaggatgaccc
caaaagagaaggtgggattgattatcaagcaggagacacttacgtgtcca
cttcagactacaaggacaactttggTtcctcttctttctcttctgctcct
tatgttagcaatgatggtctaggaagcagttctgctccaccactagaacg
acgtagtttcattggaaattcagatattcagtctttggataactacagta
ctgcttcatctcattctggtggtccacctcagccttctgccttttctgag
tcatttcccagttcagtagctggatatggagacatttacctcaatgctgg
tcccatgttgtttagtggagacaaggaccatagatttgaatatagccatg
gtcctattgcagtcctggcaaacagcagtgacccttccacggggccagag
agtactcatcctttgccagcaaagatgcacaactataactatggtggtaa
cttacaggaaaatccgagtggccccagcctcatgcatggacagacctgga
cttctcctgcccaaggacctggatattcacaaggatacaggggacatatt
agcacatcaactggcagaggcagaggcagagggttaccatactgagcggc
cgc

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMPSSSDTALGGGGGLSWAEKRLEERRK
RRRFLSPQQPPLLLPLLQPQLLQPPPPPPPLLFLAAPGTAAAAAAAAAAS
SSCFSPGPPLEVKRLARGKRRAGGRQKRRRGPRAGQEAEKRRVFSLPQPQ
QDGGGGASSGGGVTPLVEYEDVSSQSEQGLLLGGASAATAATAAGGTGGS
GGSPASSSGTQRRGEGSERRPRRDRRSSSGRSKERHREHRRRDGQRGGSE
ASKSRSRHSHSGEERAEVAKSGSSSSSGGRRKSASATSSSSSSRKDRDSK
AHRSRTKSSKEPPSAYKEPPKAYREDKTEPKAYRRRRSLSPLGGRDDSPV
SHRASQSLRSRKSPSPAGGGSSPYSRRLPRSPSPYSRRRSPSYSRHSSYE
RGGDVSPSPYSSSSWRRSRSPYSPVLRRSGKSRSRSPYSSRHSRSRSRHR
LSRSRSRHSSISPSTLTLKSSLAAELNKNKKARAAEAARAAEAAKAAEAT
KAAEAAAKAAKASNTSTPTKGNTETSASASQTNHVKDVKKIKIEHAPSPS
SGGTLKNDKAKTKPPLQVTKVENNLIVDKATKKAVIVGKESKSAATKEES
VSLKEKTKPLTPSIGAKEKEQHVALVTSTLPPLPLPPMLPEDKEADSLRG
NISVKAVKKEVEKKLRCLLADLPLPPELPGGDDLSKSPEEKKTTTQLHSK
RRPKICGPRYGETKEKDIDWGKLCVDKFDIIGIIGEGTYGQVYKARDKDT
GEMVALKKVRLDNEKEGFPITAIREIKILRQLTHQSIINMKEIVTDKEDA
LDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFNENHIKSFMRQLMEGLDY
CHKKNFLHRDIKCSNILLNNRGQIKLADFGLARLYSSEESRPYTNKVITL
WYRPPELLLGEERYTPAIDVWSCGCILGELFTKKPIFQANQELAQLELIS
RICGSPCPAVWPDVIKLPYFNTMKPKKQYRRKLREEFVFIPAAALDLFDY
MLALDPSKRCTAEQALQCEFLRDVEPSKMPPPDLPLWQDCHELWSKKRRR
QKQMGMTDDVSTIKAPRKDLSLGLDDSRTNTPQGVLPSSQLKSQGSSNVA
PVKTGPGQHLNHSELAILLNLLQSKTSVNMADFVQVLNIKVNSETQQQLN
KINLPAGILATGEKQTDPSTPQQESSKPLGGIQPSSQTIQPKMETDAAQA
AVQSAFAVLLTQLIKAQQSKQKDVLLEERENGSGHEASLQLRPPPEPSTP
VSGQDDLIQHQDMRILELTPEPDRPRILPPDQRPPEPPEPPPVTEEDLDY
RTENQHVPTTSSSLTDPHAGVKAALLQLLAQHQPQDDPKREGGIDYQAGD
TYVSTSDYKDNFGSSSFSSAPYVSNDGLGSSSAPPLERRSFIGNSDIQSL
DNYSTASSHSGGPPQPSAFSESFPSSVAGYGDIYLNAGPMLFSGDKDHRF
EYSHGPIAVLANSSDPSTGPESTHPLPAKMHNYNYGGNLQENPSGPSLMH
GQTWTSPAQGPGYSQGYRGHISTSTGRGRGRGLPY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU46146
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: MW of product 167408 Da