cDNA Clone CLASP1

• Synonyms: MAST1
• Plasmid: pGEX6P2 CLASP1 tv3
• Parent Plasmid: pGEX6P2
• Expressed: GST-3C-CLASP1 tv3
• Genbank: NM_001142274.1
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCAATGGAGCCTCGCATGGAGTCCTGCCTGGCGCAGGTGTTGCAGAAGGATGTGGGGAAACGATTGCAGGTTGGCCAAGAACTGATAGACTATTTCTCAGACAAACAGAAGTCTGCTGACCTTGAGCATGACCAGACCATGTTAGATAAACTTGTGGATGGACTTGCTACCTCTTGGGTGAACTCTAGCAATTACAAGGTGGTTCTGCTGGGCATGGACATCCTGTCCGCCCTGGTGACCCGGCTGCAGGATCGGTTCAAGGCGCAGATCGGCACAGTGCTGCCAAGTCTAATAGACAGACTAGGAGATGCTAAAGACTCTGTGAGGGAGCAGGACCAAACTCTGCTGCTAAAGATCATGGATCAAGCTGCTAATCCCCAGTACGTATGGGACAGAATGCTTGGAGGCTTCAAACACAAGAATTTCCGTACTCGAGAAGGCATCTGTCTCTGCCTTATAGCAACACTCAATGCCTCTGGAGCACAGACTTTAACACTAAGCAAGATTGTGCCACATATATGCAACTTACTTGGAGATCCAAACAGCCAGGTTCGAGATGCAGCAATAAACAGCTTAGTGGAAATTTACAGACATGTAGGAGAACGTGTGAGGGCAGATCTCAGTAAAAAAGGATTGCCACAGTCCCGGTTGAATGTAATTTTTACAAAATTTGATGAAGTCCAGAAATCTGGAAACATGATACAATCTGCAAATGATAAAAATTTCGACGATGAAGATTCTGTGGATGGTAACAGACCTTCCTCTGCTAGTTCTACATCATCCAAGGCTCCACCAAGTTCTCGGAGAAACGTTGGAATGGGAACCACCCGCCGGCTTGGTTCATCCACCCTTGGATCCAAGTCTTCAGCTGCAAAAGAAGGAGCTGGTGCTGTTGATGAAGAGGATTTTATTAAAGCATTTGATGATGTACCTGTAGTACAGATTTATTCCAGCCGAGACCTTGAGGAATCCATAAACAAAATTAGGGAAATATTATCTGATGACAAGCATGATTGGGAGCAGAGAGTAAATGCTCTAAAAAAGATTAGATCTTTACTTTTGGCTGGTGCTGCTGAGTATGATAACTTCTTTCAACATTTGCGTCTTTTGGATGGAGCCTTTAAACTCTCTGCTAAGGACCTGCGGTCTCAGGTAGTGCGGGAGGCTTGTATCACGTTGGGGCATCTGTCATCAGTTCTGGGGAATAAGTTTGACCATGGAGCTGAAGCCATTATGCCAACTATCTTTAATTTAATTCCAAACAGTGCCAAAATTATGGCCACATCTGGTGTTGTAGCTGTTAGGTTAATTATTCGGCACACACACATCCCTAGGTTAATACCTGTCATAACAAGCAACTGTACCTCTAAGTCTGTCGCAGTTAGAAGGCGCTGTTTTGAATTTTTAGATTTGCTTTTACAAGAATGGCAGACACATTCACTAGAACGACACATATCAGTATTAGCTGAAACAATAAAGAAGGGAATACATGATGCTGATTCCGAAGCAAGAATAGAAGCCAGAAAATGTTACTGGGGTTTCCACAGTCACTTCAGCAGAGAAGCAGAGCACTTGTACCACACCTTGGAGTCCTCCTACCAGAAAGCCCTGCAGTCCCACCTGAAGAACTCAGACAGCATAGTGTCTCTGCCTCAGTCAGACCGCTCATCTTCCAGCTCTCAAGAGAGTCTAAATCGTCCGCTGTCTGCCAAAAGAAGTCCTACTGGAAGTACCACATCTAGAGCTTCTACAGTTAGTACCAAATCTGTGTCAACGACTGGGTCCCTCCAGCGATCTCGAAGTGATATTGATGTGAACGCAGCAGCCAGTGCCAAATCCAAAGTCTCCTCATCTTCGGGCACGACGCCTTTCAGCTCTGCAGCAGCTTTGCCTCCAGGGTCATACGCATCCTTAGGTCGGATCCGCACAAGACGGCAAAGCTCTGGGAGTGCCACCAACGTCGCCTCTACACCTGATAACCGGGGCCGCAGTCGCGCTAAAGTGGTTTCACAGTCCCAGCGATCCAGATCTGCTAATCCTGCTGGTGCTGGCAGCCGGTCAAGTTCCCCAGGAAAATTGTTGGGAAGTGGTTATGGTGGACTTACTGGGGGCTCCTCACGAGGCCCACCTGTGACACCGTCTTCAGAAAAGCGAAGCAAGATTCCCAGGAGCCAGGGATGTAGCCGGGAAACAAGTCCAAACCGAATAGGATTAGATCGGTTTGGGCTTGGCCAGCCAGGAAGAATACCTGGTTCTGTGAATGCCATGAGAGTTCTGAGCACAAGTACAGATCTTGAAGCTGCTGTTGCTGATGCTTTGCTGTTAGGAGACTCCAGGAGCAAGAAGAAGCCTGTGAGGAGGAGATATGAGCCGTATGGGATGTATTCTGACGATGATGCCAACAGTGATGCCTCAAGTGTTTGCTCTGAGCGCTCATATGGCTCCAGGAATGGTGGCATTCCCCATTATCTGCGGCAGACTGAGGATGTAGCAGAAGTTCTCAACCACTGTGCTAGTTCAAACTGGTCAGAAAGGAAAGAAGGGCTTCTGGGCCTGCAGAACTTACTGAAGAGCCAAAGAACACTGAGTCGAGTTGAACTGAAAAGGTTGTGTGAGATCTTCACTCGGATGTTTGCTGACCCTCATAGCAAGAGAGTTTTCAGTATGTTTTTGGAGACTCTTGTGGATTTTATAATAATTCATAAGGATGATTTACAAGACTGGCTTTTTGTTCTTCTCACACAATTACTTAAGAAAATGGGAGCAGATTTACTTGGATCTGTGCAAGCAAAAGTTCAAAAGGCTCTAGATGTCACAAGGGACTCCTTTCCATTTGATCAACAATTTAACATTTTGATGAGATTTATTGTGGATCAAACTCAAACTCCAAACCTCAAGGTCAAAGTTGCAATCCTGAAATACATTGAGTCTCTGGCCAGACAGATGGATCCAACAGATTTTGTAAACTCTAGTGAGACAAGGCTTGCTGTTTCTAGAATCATAACCTGGACAACAGAACCAAAGAGTTCAGACGTGAGAAAGGCAGCACAGATTGTGCTAATCTCTCTGTTTGAATTGAATACTCCTGAATTTACCATGTTACTTGGTGCCTTGCCAAAAACATTCCAGGATGGTGCCACCAAACTCCTGCACAACCACCTCAAGAATTCCAGTAACACCAGTGTGGGCTCTCCAAGCAATACGATTGGCCGGACGCCCTCCCGACACACCAGCAGCAGGACCAGCCCCCTGACCTCACCCACCAACTGTTCCCATGGGGGTCTGTCTCCAAGCATGCTGGACTATGATACAGAGAACCTGAACTCTGAAGAAATCTATAGTTCTCTACGTGGAGTTACAGAAGCCATTGAAAAGTTTAGTTTTCGAAGCCAAGAAGATCTGAATGAGCCAATTAAACGAGATGGCAAAAAGGAGTGTGATATTGTGTCCCGCGATGGGGGCGCTGCCTCCCCTGCCACTGAGGGCCGGGGGGGTAGTGAAGTAGAAGGAGGCCGGACAGCTCTGGATAACAAGACCTCACTACTCAACACCCAGCCTCCGCGCGCCTTCCCGGGGCCGCGGGCGCGAGACTACAACCCGTACCCCTACTCAGATGCCATCAACACCTACGACAAGACCGCCCTGAAAGAGGCTGTGTTCGATGACGACATGGAGCAGCTTCGAGACGTGCCCATCGACCATTCTGACCTGGTGGCTGACCTTCTGAAAGAGCTGTCCAACCACAATGAGCGAGTGGAGGAACGGAAGGGAGCCCTGCTGGAGCTGCTCAAGATCACGCGGGAAGACAGCCTTGGTGTCTGGGAGGAGCACTTCAAGACCATTCTGCTCCTGCTGCTGGAGACCCTTGGAGACAAAGACCATTCAATTCGAGCACTGGCGTTAAGAGTTTTGAGGGAAATTCTGAGAAATCAACCAGCAAGATTTAAAAACTACGCCGAGCTGACGATTATGAAGACTCTGGAAGCCCACAAAGACTCCCATAAGGAGGTGGTGAGAGCGGCTGAGGAGGCTGCGTCCACACTGGCCAGTTCCATCCACCCGGAGCAGTGCATCAAGGTGCTCTGCCCCATCATCCAGACGGCCGACTACCCCATCAACCTTGCTGCCATCAAGATGCAGACCAAAGTCGTCGAGAGGATCGCAAAGGAGTCATTGCTGCAGCTCCTTGTCGACATCATCCCAGGCTTGCTGCAGGGTTATGACAACACCGAAAGTAGTGTGCGTAAGGCCAGCGTGTTTTGCTTAGTGGCAATTTATTCCGTAATCGGAGAAGACCTGAAACCTCACCTTGCACAGCTCACAGGGAGCAAGATGAAGCTACTAAACTTATACATAAAGAGGGCCCAGACCACCAACAGCAACAGCAGCTCCTCCTCCGATGTCTCCACGCACAGCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEIPGSTRAAAMEPRMESC
LAQVLQKDVGKRLQVGQELIDYFSDKQKSADLEHDQTMLDKLVDGLATSW
VNSSNYKVVLLGMDILSALVTRLQDRFKAQIGTVLPSLIDRLGDAKDSVR
EQDQTLLLKIMDQAANPQYVWDRMLGGFKHKNFRTREGICLCLIATLNAS
GAQTLTLSKIVPHICNLLGDPNSQVRDAAINSLVEIYRHVGERVRADLSK
KGLPQSRLNVIFTKFDEVQKSGNMIQSANDKNFDDEDSVDGNRPSSASST
SSKAPPSSRRNVGMGTTRRLGSSTLGSKSSAAKEGAGAVDEEDFIKAFDD
VPVVQIYSSRDLEESINKIREILSDDKHDWEQRVNALKKIRSLLLAGAAE
YDNFFQHLRLLDGAFKLSAKDLRSQVVREACITLGHLSSVLGNKFDHGAE
AIMPTIFNLIPNSAKIMATSGVVAVRLIIRHTHIPRLIPVITSNCTSKSV
AVRRRCFEFLDLLLQEWQTHSLERHISVLAETIKKGIHDADSEARIEARK
CYWGFHSHFSREAEHLYHTLESSYQKALQSHLKNSDSIVSLPQSDRSSSS
SQESLNRPLSAKRSPTGSTTSRASTVSTKSVSTTGSLQRSRSDIDVNAAA
SAKSKVSSSSGTTPFSSAAALPPGSYASLGRIRTRRQSSGSATNVASTPD
NRGRSRAKVVSQSQRSRSANPAGAGSRSSSPGKLLGSGYGGLTGGSSRGP
PVTPSSEKRSKIPRSQGCSRETSPNRIGLDRFGLGQPGRIPGSVNAMRVL
STSTDLEAAVADALLLGDSRSKKKPVRRRYEPYGMYSDDDANSDASSVCS
ERSYGSRNGGIPHYLRQTEDVAEVLNHCASSNWSERKEGLLGLQNLLKSQ
RTLSRVELKRLCEIFTRMFADPHSKRVFSMFLETLVDFIIIHKDDLQDWL
FVLLTQLLKKMGADLLGSVQAKVQKALDVTRDSFPFDQQFNILMRFIVDQ
TQTPNLKVKVAILKYIESLARQMDPTDFVNSSETRLAVSRIITWTTEPKS
SDVRKAAQIVLISLFELNTPEFTMLLGALPKTFQDGATKLLHNHLKNSSN
TSVGSPSNTIGRTPSRHTSSRTSPLTSPTNCSHGGLSPSMLDYDTENLNS
EEIYSSLRGVTEAIEKFSFRSQEDLNEPIKRDGKKECDIVSRDGGAASPA
TEGRGGSEVEGGRTALDNKTSLLNTQPPRAFPGPRARDYNPYPYSDAINT
YDKTALKEAVFDDDMEQLRDVPIDHSDLVADLLKELSNHNERVEERKGAL
LELLKITREDSLGVWEEHFKTILLLLLETLGDKDHSIRALALRVLREILR
NQPARFKNYAELTIMKTLEAHKDSHKEVVRAAEEAASTLASSIHPEQCIK
VLCPIIQTADYPINLAAIKMQTKVVERIAKESLLQLLVDIIPGLLQGYDN
TESSVRKASVFCLVAIYSVIGEDLKPHLAQLTGSKMKLLNLYIKRAQTTN
SNSSSSSDVSTHS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU48820
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: variant 3