cDNA Clone ITSN1

• Plasmid: pCMV5D HA ITSN1
• Parent Plasmid: pCMV5D HA
• Expressed: HA ITSN1
• Genbank: 6453
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCTTCGAAGAATTCGCTAGCCTCGAGATGGCTCAGTTTCCAACACCTTTTGGTGGCAGCCTGGATATCTGGGCCATAACTGTAGAGGAAAGAGCGAAGCATGATCAGCAGTTCCATAGTTTAAAGCCAATATCTGGATTCATTACTGGTGATCAAGCTAGAAACTTTTTTTTTCAATCTGGGTTACCTCAACCTGTTTTAGCACAGATATGGGCACTAGCTGACATGAATAATGATGGAAGAATGGATCAAGTGGAGTTTTCCATAGCTATGAAACTTATCAAACTGAAGCTACAAGGATATCAGCTACCCTCTGCACTTCCCCCTGTCATGAAACAGCAACCAGTTGCTATTTCTAGCGCACCAGCATTTGGTATGGGAGGTATCGCCAGCATGCCACCGCTTACAGCTGTTGCTCCAGTGCCAATGGGATCCATTCCAGTTGTTGGAATGTCTCCAACCCTAGTATCTTCTGTTCCCACAGCAGCTGTGCCCCCCCTGGCTAACGGGGCTCCCCCTGTTATACAACCTCTGCCTGCATTTGCTCATCCTGCAGCCACATTGCCAAAGAGTTCTTCCTTTAGTAGATCTGGTCCAGGGTCACAACTAAACACTAAATTACAAAAGGCACAGTCATTTGATGTGGCCAGTGTCCCACCAGTGGCAGAGTGGGCTGTTCCTCAGTCATCAAGACTGAAATACAGGCAATTATTCAATAGTCATGACAAAACTATGAGTGGACACTTAACAGGTCCCCAAGCAAGAACTATTCTTATGCAGTCAAGTTTACCACAGGCTCAGCTGGCTTCAATATGGAATCTTTCTGACATTGATCAAGATGGAAAACTTACAGCAGAGGAATTTATCCTGGCAATGCACCTCATTGATGTAGCTATGTCTGGCCAACCACTGCCACCTGTCCTGCCTCCAGAATACATTCCACCTTCTTTTAGAAGAGTTCGATCTGGCAGTGGTATATCTGTCATAAGCTCAACATCTGTAGATCAGAGGCTACCAGAGGAACCAGTTTTAGAAGATGAACAACAACAATTAGAAAAGAAATTACCTGTAACGTTTGAAGATAAGAAGCGGGAGAACTTTGAACGTGGCAACCTGGAACTGGAGAAACGAAGGCAAGCTCTCCTGGAACAGCAGCGCAAGGAGCAGGAGCGCCTGGCCCAGCTGGAGCGGGCGGAGCAGGAGAGGAAGGAGCGTGAGCGCCAGGAGCAAGAGCGCAAAAGACAACTGGAACTGGAGAAGCAACTGGAAAAGCAGCGGGAGCTAGAACGGCAGAGAGAGGAGGAGAGGAGGAAAGAAATTGAGAGGCGAGAGGCTGCAAAACGGGAACTTGAAAGGCAACGACAACTTGAGTGGGAACGGAATCGAAGGCAAGAACTACTAAATCAAAGAAACAAAGAACAAGAGGACATAGTTGTACTGAAAGCAAAGAAAAAGACTTTGGAATTTGAATTAGAAGCTCTAAATGATAAAAAGCATCAACTAGAAGGGAAACTTCAAGATATCAGATGTCGATTGACCACCCAAAGGCAAGAAATTGAGAGCACAAACAAATCTAGAGAGTTGAGAATTGCCGAAATCACCCATCTACAGCAACAATTACAGGAATCTCAGCAAATGCTTGGAAGACTTATTCCAGAAAAACAGATACTCAATGACCAATTAAAACAAGTTCAGCAGAACAGTTTGCACAGAGATTCACTTGTTACACTTAAAAGAGCCTTAGAAGCAAAAGAACTAGCTCGGCAGCACCTACGAGACCAACTGGATGAAGTGGAGAAAGAAACTAGATCAAAACTACAGGAGATTGATATTTTCAATAATCAGCTGAAGGAACTAAGAGAAATACACAATAAGCAACAACTCCAGAAGCAAAAGTCCATGGAGGCTGAACGACTGAAACAGAAAGAACAAGAACGAAAGATCATAGAATTAGAAAAACAAAAAGAAGAAGCCCAAAGACGAGCTCAGGAAAGGGACAAGCAGTGGCTGGAGCATGTGCAGCAGGAGGACGAGCATCAGAGACCAAGAAAACTCCACGAAGAGGAAAAACTGAAAAGGGAGGAGAGTGTCAAAAAGAAGGATGGCGAGGAAAAAGGCAAACAGGAAGCACAAGACAAGCTGGGTCGGCTTTTCCATCAACACCAAGAACCAGCTAAGCCAGCTGTCCAGGCACCCTGGTCCACTGCAGAAAAAGGTCCACTTACCATTTCTGCACAGGAAAATGTAAAAGTGGTGTATTACCGGGCACTGTACCCCTTTGAATCCAGAAGCCATGATGAAATCACTATCCAGCCAGGAGACATAGTCATGGTTAAAGGGGAATGGGTGGATGAAAGCCAAACTGGAGAACCCGGCTGGCTTGGAGGAGAATTAAAAGGAAAGACAGGGTGGTTCCCTGCAAACTATGCAGAGAAAATCCCAGAAAATGAGGTTCCCGCTCCAGTGAAACCAGTGACTGATTCAACATCTGCCCCTGCCCCCAAACTGGCCTTGCGTGAGACCCCCGCCCCTTTGGCAGTAACCTCTTCAGAGCCCTCCACGACCCCTAATAACTGGGCCGACTTCAGCTCCACGTGGCCCACCAGCACGAATGAGAAACCAGAAACGGATAACTGGGATGCATGGGCAGCCCAGCCCTCTCTCACCGTTCCAAGTGCCGGCCAGTTAAGGCAGAGGTCCGCCTTTACTCCAGCCACGGCCACTGGCTCCTCCCCGTCTCCTGTGCTAGGCCAGGGTGAAAAGGTGGAGGGGCTACAAGCTCAAGCCCTATATCCTTGGAGAGCCAAAAAAGACAACCACTTAAATTTTAACAAAAATGATGTCATCACCGTCCTGGAACAGCAAGACATGTGGTGGTTTGGAGAAGTTCAAGGTCAGAAGGGTTGGTTCCCCAAGTCTTACGTGAAACTCATTTCAGGGCCCATAAGGAAGTCTACAAGCATGGATTCTGGTTCTTCAGAGAGTCCTGCTAGTCTAAAGCGAGTAGCCTCTCCAGCAGCCAAGCCGGTCGTTTCGGGAGAAGAATTTATTGCCATGTACACTTACGAGAGTTCTGAGCAAGGAGATTTAACCTTTCAGCAAGGGGATGTGATTTTGGTTACCAAGAAAGATGGTGACTGGTGGACAGGAACAGTGGGCGACAAGGCCGGAGTCTTCCCTTCTAACTATGTGAGGCTTAAAGATTCAGAGGGCTCTGGAACTGCTGGGAAAACAGGGAGTTTAGGAAAAAAACCTGAAATTGCCCAGGTTATTGCCTCATACACCGCCACCGGCCCCGAGCAGCTCACTCTCGCCCCTGGTCAGCTGATTTTGATCCGAAAAAAGAACCCAGGTGGATGGTGGGAAGGAGAGCTGCAAGCACGTGGGAAAAAGCGCCAGATAGGCTGGTTCCCAGCTAATTATGTAAAGCTTCTAAGCCCTGGGACGAGCAAAATCACTCCAACAGAGCCACCTAAGTCAACAGCATTAGCGGCAGTGTGCCAGGTGATTGGGATGTACGACTACACCGCGCAGAATGACGATGAGCTGGCCTTCAACAAGGGCCAGATCATCAACGTCCTCAACAAGGAGGACCCTGACTGGTGGAAAGGAGAAGTCAATGGACAAGTGGGGCTCTTCCCATCCAATTATGTGAAGCTGACCACAGACATGGACCCAAGCCAGCAATGGTGTTCAGACTTACATCTCTTGGATATGTTGACCCCAACTGAAAGAAAGCGACAAGGATACATCCACGAGCTCATTGTCACCGAGGAGAACTATGTGAATGACCTGCAGCTGGTCACAGAGATTTTTCAAAAACCCCTGATGGAGTCTGAGCTGCTGACAGAAAAAGAGGTTGCTATGATTTTTGTGAACTGGAAGGAGCTGATTATGTGTAATATCAAACTACTAAAAGCGCTGAGAGTCCGCAAGAAGATGTCCGGGGAGAAGATGCCTGTGAAGATGATTGGAGACATCCTGAGCGCACAGCTGCCGCACATGCAGCCCTACATCCGCTTCTGCAGCCGCCAGCTCAACGGGGCTGCCCTGATCCAGCAGAAGACGGACGAGGCCCCAGACTTCAAGGAGTTCGTCAAAAGATTGGCAATGGATCCTCGGTGTAGAGGGATGCCACTCTCTAGTTTTATACTGAAGCCTATGCAACGGGTAACAAGATACCCACTGATCATTAAAAATATCCTGGAAAACACCCCTGAAAACCACCCGGACCACAGCCACTTGAAGCACGCCCTGGAGAAGGCGGAAGAGCTCTGTTCCCAGGTGAACGAAGGGGTGCGGGAGAAGGAGAACTCTGACCGGCTGGAGTGGATCCAGGCCCACGTGCAGTGTGAAGGCCTGTCTGAGCAACTTGTGTTCAATTCAGTGACCAATTGCTTGGGGCCGCGCAAATTTCTGCACAGTGGGAAGCTCTACAAGGCCAAGAGCAACAAGGAGCTGTATGGCTTCCTTTTCAACGACTTCCTCCTGCTGACTCAGATCACGAAGCCTTTGGGGTCTTCTGGCACCGACAAAGTCTTCAGCCCCAAATCAAACCTGCAGTATAAAATGTATAAAACACCTATTTTCCTAAATGAGGTTCTAGTAAAATTACCCACCGACCCTTCTGGAGACGAGCCCATCTTCCACATCTCCCACATTGACCGCGTCTATACTCTCCGAGCAGAAAGCATAAATGAAAGGACTGCCTGGGTGCAGAAAATCAAAGCTGCTTCTGAACTCTACATAGAGACTGAGAAAAAGAAGCGCGAGAAAGCGTACCTGGTCCGTTCCCAAAGGGCAACAGGCATTGGAAGGTTGATGGTGAACGTGGTTGAAGGCATCGAGTTGAAACCCTGTCGGTCACATGGAAAGAGCAACCCGTACTGTGAGGTGACCATGGGTTCCCAGTGCCACATCACCAAGACGATCCAGGACACTCTGAACCCCAAGTGGAATTCCAACTGCCAGTTCTTCATCCGAGACCTGGAGCAGGAAGTCCTCTGCATCACTGTGTTCGAGAGGGACCAGTTCTCACCAGATGATTTTTTGGGTCGGACGGAGATCCGTGTGGCGGACATCAAGAAAGACCAGGGCTCCAAAGGTCCAGTTACGAAGTGTCTTCTGCTGCACGAAGTCCCCACGGGAGAGATTGTGGTCCGCTTGGACCTGCAGTTGTTTGATGAGCCGTAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSFEEFASLEMAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCRGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU27053
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: THis clone has 1 silent mutation D1348 and 1 naturally occuring SNP K1367R
• Low Temp: No