Plasmid
pcDNA5D FRT/TO GFP DENND4C HA
Parent Plasmid
pcDNA5D FRT/TO GFP
Expressed
GFP DENND4C HA
Genbank
NM_017925.6
Tag
HA
GFP
Sequence of Insert
View Sequence
CTCGAGATGATAGAAGACAAAGGACCAAGAGTGACAGACTACTTTGTCGTAGCTGGTCTCACTGACACATCTACTCTTTTGGATCAAGAAATAAATCGTTTAGATACTAAGTCAACTGGACCTAAAGCTCCAATTACAGACATTGCCATTATTATCAAATCAGCTGGAGAAACAGTACCTGAAGGTTACACCTGTGTAGAAGCCACTCCATCAGCTCTCCAAGCAAACTTGAACTATGGAAGTCTGAAAAGCCCAGAACTTTTCCTCTGTTATAAGAGAGGGAGAGATAAACCACCGCTTACAGATATTGGAGTTCTATATGAAGGGAAAGAACGGCTTATTCCAGGATGTGAAGTGATCCTAGCCACACCCTATGGTCGCTGTGCCAATGTCAACAATAGTTCAACTACTTCACAAAGAATCTTTATCACTTATCGAAGGGCTCCTCCAGTTCGACCCCAGAATTCCTTGGCTGTAACTGATATCTGTGTTATTGTAACCAGTAAAGGAGAAACTCCTCCTCATACCTTCTGCAAAGTTGACAAAAACTTAAATTGTGGAATGTGGGGTTCCAGCGTGTTTCTGTGTTATAAGAAGTCTGTACCTGCTTCAAATGCAATAGCATATAAGGCTGGTTTAATTTTTAGATATCCAGAAGAGGACTATGAGTCATTTCCACTCTCAGAATCAGATGTACCTCTTTTCTGCCTTCCTATGGGAGCTACTATTGAGTGCTGGGATCCTGAAACCAAATATCCACTTCCAGTTTTTTCAACTTTTGTCTTGACAGGTTCTTCAGCCAAAAAGGTATATGGAGCTGCCATTCAGTTTTATGAACCTTACTCTCGGGAACTTCTATCAGAGAAACAGCTTATGCACCTGGGCTTGTTGACGCCTGTGGAGAGAAAAATGGTCTCCAAATCCATCAATACAAACAAATGCATTTGTTTACTCTCACACTGGCCTTTTTTTGAAGCTTTTAGGAAATTTCTTATGTTTATCTACAAACTTTCTGTGTCTGGACCACATCCTCTTCCCATTGAAAAGCACATTTCACATTTTATGCAAAACATCCCTTTTCCTTCACCACAAAGACCGAGAATCCTTGTCCAGCTGTCAGTCCATGATGCATTAATATTATCACAGCCAGTTTCTACACCTTTACCACTAAGTGGAGCCAACTTTAGCACCTTGCTAATGAATCTGGGTCCTGAGAATTGTGCAACACTGCTGCTCTTTGTTTTACTTGAGAGTAAAATTCTGCTGCATTCTCTTAGGCCAGCTGTCTTGACTGGGGTAGCTGAAGCTGTTGTAGCTATGATCTTTCCATTTCAGTGGCAATGCCCATATATTCCCCTTTGTCCTCTTTCACTGGCTGCAGTGCTTAGTGCACCTTTACCATTTATAGTTGGAGTTGACTCAAGGTATTTTGATCTTCATGACCCACCACAAGATGTTGTTTGCATTGACTTGGATACGAACATGTTATATGTATCAGATGAAAAGAAGAACATGAACTGGAAGCAACTTCCCAAAAAGCCGTGCAAAAATCTACTTAGCACCTTAAAGAAATTGTATCCCCAGCTGTCTTCAGTTCACCAAAAAACTCAAGAAGGCTCAGCGATTGACATGACTCCAATTGAAGCAGATTTCTCCTGGCAAAAGAAGATGACACAGCTTGAGATGGAAATTCAAGAGGCATTTTTGCGCTTTATGGCGTCTATTTTAAAAGGATATAGAACATATCTCAGACCAATCACAGAGGCTCCTTCAAATAAAGCCACAGCTGCTGATTCATTGTTTGACCGACAGGGATTTTTAAAAAGTCGAGATCGTGCCTATGCAAAATTCTATACCCTTTTATCCAAAACACAGATTTTTATTCGTTTCATTGAAGAATGCAGTTTTGTAAGTGATAAAGATACTGGATTAGCATTTTTTGATGACTGCATAGAAAAGTTGTTTCCTGATAAAGGCACAGAGAAAACAGATAAGGTTGATTTTGATTCAGCAGAAGATACCAGATTGATAGAACTAGATGATTCACAGAAAAGTGAGCATACTGTATTTATAATGCCGCCAGAGCCACCTCCTGATGATGGAAAGGACCTGTCACCAAAGTACAGTTACAAATACTTTCCAAGACTGGACCTTAAGCTTTTTGACAGACCGCAGGAGTTGAAACTTTGTTTTAGTAGACACCCTACTGGGAATAGCATTACAAAGAGTCCACCTCTCATGGCTAAGAGAACTAAACAGGAAATAAAAACAGCTCATAAATTGGCGAAGAGATGTTATACAAATCCACCACAGTGGGCCAAGTGTCTGTTTAGTCATTGTTACAGTTTATGGTTTATTTGTCTTCCGGCCTATGTTAGAGTTTCTCATCCTAAAGTCAGAGCACTTCAGCAGGCATATGATGTACTTATTAAGATGAGGAAAACAGATGTGGATCCCTTAGATGAGGTGTGCTATCGAGTAGTGATGCAGCTTTGTGGACTTTGGGGTCATCCTGTTTTAGCAGTGAGAGTCTTATTTGAAATGAAAACTGCTAGGATAAAGCCTAATGCTATTACTTATGGTTATTATAATAAGGTAGTCTTGGAGAGCCCGTGGCCTAGCAGTACCCGCAGTGGTATTTTCTTATGGACGAAGGTACGGAATGTGGTACGTGGCTTGGCACAGTTTAGGCAGCCGCTTAAAAAGACTGTGCAAAGGTCACAGGTCTCCTCAATATCAGGTGGTCAGTCTGACCAAGGATACGGGTCTAAGGATGAACTTATAAAGGATGATGCAGAAATTCATGTGCCTGAAGAACAGGCAGCAAGAGAATTGATAACTAAAACAAAAATGCAAACAGAAGAGGTGTGTGATGCCTCTGCTATTGTGGCAAAACATTCACAACCTAGTCCAGAGCCTCACAGTCCTACTGAACCTCCTGCATGGGGCAGCAGTATTGTGAAAGTTCCGTCTGGTATATTTGATGTCAACAGCAGGAAAAGTAGCACTGGTAGTATATCAAATGTGCTGTTTTCTACTCAAGATCCAGTTGAAGATGCAGTCTTTGGCGAAGCTACTAATCTCAAGAAGAATGGTGATAGAGGAGAAAAAAGACAAAAGCATTTTCCTGAGAGGAGTTGTAGTTTTAGTTCTGAAAGTCGAGCAGGAATGTTGCTTAAGAAGAGTAGTTTGGATTCGAATTCAAGTGAAATGGCTATCATGATGGGAGCAGATGCCAAGATTCTCACAGCAGCATTGACATGTCCTAAGACTTCTCTACTTCATATTGCAAGAACCCATAGCTTTGAGAATGTTAGCTGTCACCTACCTGATAGTAGGACTTGTATGTCTGAAAGCACTTGGAATCCTGAGCACAGATCATCTCCGGTGCCAGAGATGCTTGAGGAAAGCCAAGAACTCCTTGAGCCTGTGGTTGATGACGTACCTAAAACTACTGCAACAGTAGATACATATGAGAGTCTACTAAGTGATAGTAACAGTAATCAGTCCAGAGACTTGAAAACAGTATCCAAAGACCTGAGGAATAAGAGAAGTAGTTTATATGGTATTGCTAAGGTGGTTCAGAGGGAAGATGTTGAAACTGGACTAGATCCTTTGTCTCTTTTAGCCACTGAATGTACAGGAGGAAAAACTCCTGATTCTGAAGATAAGTTGTTTTCTCCAGTTATTGCACGTAATCTGGCTGATGAAATAGAAAGCTATATGAACCTAAAAAGTCCCCTAGGTAGTAAATCTTCTAGTATGGAATTACACAGAGAGGAAAACAGAGAGTCTGGCATGACTACTGCATTTATTCATGCTCTAGAGAGGAGATCAAGCCTACCTTTAGATCATGGTTCACCAGCACAGGAAAATCCTGAAAGTGAAAAGAGCTCACCTGCAGTGTCCAGGTCTAAAACTTTTACTGGGCGTTTCAAGCAGCAAACCCCCTCTCGAACTCATAAAGAACGTTCAACTTCTTTGTCAGCACTGGTGCGTTCTTCGCCACATGGCTCGTTGGGTTCTGTAGTAAATTCTTTGTCAGGGCTAAAGCTGGATAATATACTCTCAGGGCCCAAGATAGATGTCCTGAAATCTGGTATGAAACAAGCAGCGACAGTAGCCAGTAAGATGTGGGTAGCTGTTGCGTCTGCCTACAGCTACTCAGATGATGAGGAAGAAACTAATAGAGACTACAGCTTCCCAGCTGGCCTAGAAGACCATATTTTGGGGGAGAATATATCGCCTAACACAAGTATCTCAGGGTTGGTCCCCAGTGAACTTACCCAGAGCAACACAAGTCTTGGCAGTAGCAGCAGTAGTGGAGATGTAGGAAAACTGCATTATCCAACAGGTGAAGTTCCATTTCCAAGAGGCATGAAAGGGCAAGACTTTGAAAAATCAGATCATGGTTCTTCTCAAAATACCAGCATGTCTAGCATCTATCAGAATTGTGCAATGGAGGTTTTGATGTCCAGTTGTTCACAGTGTAGAGCTTGTGGAGCTTTAGTTTATGATGAAGAAATTATGGCTGGATGGACAGCAGATGACTCAAATTTGAATACAGCTTGTCCATTCTGTAAAAGCAACTTCTTGCCTCTTCTCAATATAGAATTCAAAGATTTGAGAGGTTCTGCAAGCTTTTTCCTGAAACCAAGTACCTCTGGTGACAGTTTACAAAGTGGAAGCATTCCATTGGCAAATGAATCCTTGGAGCACAAACCTGTATCCAGTTTAGCAGAACCTGACTTGATCAACTTTATGGACTTCCCAAAACATAACCAGATCATAACTGAAGAAACAGGCTCTGCAGTTGAACCAAGTGATGAAATAAAGAGAGCCAGTGGAGATGTCCAAACTATGAAAATTTCATCTGTGCCTAATAGTTTATCAAAGCGAAATGTGTCTTTGACTCGAAGTCACAGTGTTGGAGGCCCATTGCAGAATATTGACTTTACCCAGCGACCGTTTCATGGCATCTCAACAGTTAGTCTTCCAAATAGTCTGCAGGAAGTTGTGGATCCTTTAGGAAAAAGACCCAATCCTCCCCCTGTTTCTGTGCCCTACTTGAGTCCTCTAGTACTCCGTAAAGAACTTGAATCTTTGCTAGAAAATGAAGGTGATCAGGTGATTCATACATCTTCTTTCATCAATCAACATCCAATCATTTTCTGGAACCTCGTTTGGTATTTCAGACGTTTGGACCTTCCTAGTAACTTGCCAGGACTTATCCTCACATCTGAACATTGTAATGAAGGTGTACAGCTTCCTCTGTCATCTCTGTCCCAGGATAGCAAACTTGTGTATATTCAGCTGTTATGGGATAATATCAACCTTCATCAGGAACCAAGAGAACCTCTGTATGTCTCATGGAGGAATTTTAATTCTGAAAAGAAATCATCTCTCCTGTCAGAGGAACAACAAGAAACAAGCACTTTAGTAGAAACCATCAGGCAGAGTATTCAGCACAATAATGTTCTTAAACCCATCAACCTACTTTCACAGCAAATGAAGCCAGGCATGAAAAGACAAAGGAGTTTATACAGAGAAATCCTCTTCTTATCATTAGTGTCTCTAGGAAGAGAGAATATTGATATTGAGGCATTTGACAATGAATATGGAATTGCATACAATAGTCTGTCTTCAGAGATTCTTGAAAGGTTGCAGAAAATTGATGCTCCACCAAGTGCCAGTGTCGAGTGGTGCAGGAAGTGTTTTGGAGCGCCTCTCATTGCGGCCGC
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFASLEMIEDKGPRVTDYFVVAGLTDTSTLLDQEINRLDTKSTGPKAPITDIAIIIKSAGETVPEGYTCVEATPSALQANLNYGSLKSPELFLCYKRGRDKPPLTDIGVLYEGKERLIPGCEVILATPYGRCANVNNSSTTSQRIFITYRRAPPVRPQNSLAVTDICVIVTSKGETPPHTFCKVDKNLNCGMWGSSVFLCYKKSVPASNAIAYKAGLIFRYPEEDYESFPLSESDVPLFCLPMGATIECWDPETKYPLPVFSTFVLTGSSAKKVYGAAIQFYEPYSRELLSEKQLMHLGLLTPVERKMVSKSINTNKCICLLSHWPFFEAFRKFLMFIYKLSVSGPHPLPIEKHISHFMQNIPFPSPQRPRILVQLSVHDALILSQPVSTPLPLSGANFSTLLMNLGPENCATLLLFVLLESKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPYIPLCPLSLAAVLSAPLPFIVGVDSRYFDLHDPPQDVVCIDLDTNMLYVSDEKKNMNWKQLPKKPCKNLLSTLKKLYPQLSSVHQKTQEGSAIDMTPIEADFSWQKKMTQLEMEIQEAFLRFMASILKGYRTYLRPITEAPSNKATAADSLFDRQGFLKSRDRAYAKFYTLLSKTQIFIRFIEECSFVSDKDTGLAFFDDCIEKLFPDKGTEKTDKVDFDSAEDTRLIELDDSQKSEHTVFIMPPEPPPDDGKDLSPKYSYKYFPRLDLKLFDRPQELKLCFSRHPTGNSITKSPPLMAKRTKQEIKTAHKLAKRCYTNPPQWAKCLFSHCYSLWFICLPAYVRVSHPKVRALQQAYDVLIKMRKTDVDPLDEVCYRVVMQLCGLWGHPVLAVRVLFEMKTARIKPNAITYGYYNKVVLESPWPSSTRSGIFLWTKVRNVVRGLAQFRQPLKKTVQRSQVSSISGGQSDQGYGSKDELIKDDAEIHVPEEQAARELITKTKMQTEEVCDASAIVAKHSQPSPEPHSPTEPPAWGSSIVKVPSGIFDVNSRKSSTGSISNVLFSTQDPVEDAVFGEATNLKKNGDRGEKRQKHFPERSCSFSSESRAGMLLKKSSLDSNSSEMAIMMGADAKILTAALTCPKTSLLHIARTHSFENVSCHLPDSRTCMSESTWNPEHRSSPVPEMLEESQELLEPVVDDVPKTTATVDTYESLLSDSNSNQSRDLKTVSKDLRNKRSSLYGIAKVVQREDVETGLDPLSLLATECTGGKTPDSEDKLFSPVIARNLADEIESYMNLKSPLGSKSSSMELHREENRESGMTTAFIHALERRSSLPLDHGSPAQENPESEKSSPAVSRSKTFTGRFKQQTPSRTHKERSTSLSALVRSSPHGSLGSVVNSLSGLKLDNILSGPKIDVLKSGMKQAATVASKMWVAVASAYSYSDDEEETNRDYSFPAGLEDHILGENISPNTSISGLVPSELTQSNTSLGSSSSSGDVGKLHYPTGEVPFPRGMKGQDFEKSDHGSSQNTSMSSIYQNCAMEVLMSSCSQCRACGALVYDEEIMAGWTADDSNLNTACPFCKSNFLPLLNIEFKDLRGSASFFLKPSTSGDSLQSGSIPLANESLEHKPVSSLAEPDLINFMDFPKHNQIITEETGSAVEPSDEIKRASGDVQTMKISSVPNSLSKRNVSLTRSHSVGGPLQNIDFTQRPFHGISTVSLPNSLQEVVDPLGKRPNPPPVSVPYLSPLVLRKELESLLENEGDQVIHTSSFINQHPIIFWNLVWYFRRLDLPSNLPGLILTSEHCNEGVQLPLSSLSQDSKLVYIQLLWDNINLHQEPREPLYVSWRNFNSEKKSSLLSEEQQETSTLVETIRQSIQHNNVLKPINLLSQQMKPGMKRQRSLYREILFLSLVSLGRENIDIEAFDNEYGIAYNSLSSEILERLQKIDAPPSASVEWCRKCFGAPLIAAAYPYDVPDYA*
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU66185
Unit Source
Location
PPU
MTA
Notes
By mistake cloned into pcDNA FRT TO GFP rather than c-ter GFP, taken over from Mel 01/05/2019
Low Temp
No
Price
£125.00
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