cDNA Clone DENND4C

• Synonyms: bA513M16.3; C9orf55; C9orf55B; RAB10GEF
• Plasmid: pcDNA5D FRT/TO GFP DENND4C isoform 1
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP DENND4C isoform 1
• Genbank: NM_017925.6 with one silent change
• Tag: GFP
• Species: Human

Sequence of Insert

CTCGAGATGATAGAAGACAAAGGACCAAGAGTGACAGACTACTTTGTCGTAGCTGGTCTCACTGACACATCTACTCTTTTGGATCAAGAAATAAATCGTTTAGATACTAAGTCAACTGGACCTAAAGCTCCAATTACAGACATTGCCATTATTATCAAATCAGCTGGAGAAACAGTACCTGAAGGTTACACCTGTGTAGAAGCCACTCCATCAGCTCTCCAAGCAAACTTGAACTATGGAAGTCTGAAAAGCCCAGAACTTTTCCTCTGTTATAAGAGAGGGAGAGATAAACCACCGCTTACAGATATTGGAGTTCTATATGAAGGGAAAGAACGGCTTATTCCAGGATGTGAAGTGATCCTAGCCACACCCTATGGTCGCTGTGCCAATGTCAACAATAGTTCAACTACTTCACAAAGAATCTTTATCACTTATCGAAGGGCTCCTCCAGTTCGACCCCAGAATTCCTTGGCTGTAACTGATATCTGTGTTATTGTAACCAGTAAAGGAGAAACTCCTCCTCATACCTTCTGCAAAGTTGACAAAAACTTAAATTGTGGAATGTGGGGTTCCAGCGTGTTTCTGTGTTATAAGAAGTCTGTACCTGCTTCAAATGCAATAGCATATAAGGCTGGTTTAATTTTTAGATATCCAGAAGAGGACTATGAGTCATTTCCACTCTCAGAATCAGATGTACCTCTTTTCTGCCTTCCTATGGGAGCTACTATTGAGTGCTGGGATCCTGAAACCAAATATCCACTTCCAGTTTTTTCAACTTTTGTCTTGACAGGTTCTTCAGCCAAAAAGGTATATGGAGCTGCCATTCAGTTTTATGAACCTTACTCTCGGGAACTTCTATCAGAGAAACAGCTTATGCACCTGGGCTTGTTGACGCCTGTGGAGAGAAAAATGGTCTCCAAATCCATCAATACAAACAAATGCATTTGTTTACTCTCACACTGGCCTTTTTTTGAAGCTTTTAGGAAATTTCTTATGTTTATCTACAAACTTTCTGTGTCTGGACCACATCCTCTTCCCATTGAAAAGCACATTTCACATTTTATGCAAAACATCCCTTTTCCTTCACCACAAAGACCGAGAATCCTTGTCCAGCTGTCAGTCCATGATGCATTAATATTATCACAGCCAGTTTCTACACCTTTACCACTAAGTGGAGCCAACTTTAGCACCTTGCTAATGAATCTGGGTCCTGAGAATTGTGCAACACTGCTGCTCTTTGTTTTACTTGAGAGTAAAATTCTGCTGCATTCTCTTAGGCCAGCTGTCTTGACTGGGGTAGCTGAAGCTGTTGTAGCTATGATCTTTCCATTTCAGTGGCAATGCCCATATATTCCCCTTTGTCCTCTTTCACTGGCTGCAGTGCTTAGTGCACCTTTACCATTTATAGTTGGAGTTGACTCAAGGTATTTTGATCTTCATGACCCACCACAAGATGTTGTTTGCATTGACTTGGATACGAACATGTTATATGTATCAGATGAAAAGAAGAACATGAACTGGAAGCAACTTCCCAAAAAGCCGTGCAAAAATCTACTTAGCACCTTAAAGAAATTGTATCCCCAGCTGTCTTCAGTTCACCAAAAAACTCAAGAAGGCTCAGCGATTGACATGACTCCAATTGAAGCAGATTTCTCCTGGCAAAAGAAGATGACACAGCTTGAGATGGAAATTCAAGAGGCATTTTTGCGCTTTATGGCGTCTATTTTAAAAGGATATAGAACATATCTCAGACCAATCACAGAGGCTCCTTCAAATAAAGCCACAGCTGCTGATTCATTGTTTGACCGACAGGGATTTTTAAAAAGTCGAGATCGTGCCTATGCAAAATTCTATACCCTTTTATCCAAAACACAGATTTTTATTCGTTTCATTGAAGAATGCAGTTTTGTAAGTGATAAAGATACTGGATTAGCATTTTTTGATGACTGCATAGAAAAGTTGTTTCCTGATAAAGGCACAGAGAAAACAGATAAGGTTGATTTTGATTCAGCAGAAGATACCAGATTGATAGAACTAGATGATTCACAGAAAAGTGAGCATACTGTATTTATAATGCCGCCAGAGCCACCTCCTGATGATGGAAAGGACCTGTCACCAAAGTACAGTTACAAATACTTTCCAAGACTGGACCTTAAGCTTTTTGACAGACCGCAGGAGTTGAAACTTTGTTTTAGTAGACACCCTACTGGGAATAGCATTACAAAGAGTCCACCTCTCATGGCTAAGAGAACTAAACAGGAAATAAAAACAGCTCATAAATTGGCGAAGAGATGTTATACAAATCCACCACAGTGGGCCAAGTGTCTGTTTAGTCATTGTTACAGTTTATGGTTTATTTGTCTTCCGGCCTATGTTAGAGTTTCTCATCCTAAAGTCAGAGCACTTCAGCAGGCATATGATGTACTTATTAAGATGAGGAAAACAGATGTGGATCCCTTAGATGAGGTGTGCTATCGAGTAGTGATGCAGCTTTGTGGACTTTGGGGTCATCCTGTTTTAGCAGTGAGAGTCTTATTTGAAATGAAAACTGCTAGGATAAAGCCTAATGCTATTACTTATGGTTATTATAATAAGGTAGTCTTGGAGAGCCCGTGGCCTAGCAGTACCCGCAGTGGTATTTTCTTATGGACGAAGGTACGGAATGTGGTACGTGGCTTGGCACAGTTTAGGCAGCCGCTTAAAAAGACTGTGCAAAGGTCACAGGTCTCCTCAATATCAGGTGGTCAGTCTGACCAAGGATACGGGTCTAAGGATGAACTTATAAAGGATGATGCAGAAATTCATGTGCCTGAAGAACAGGCAGCAAGAGAATTGATAACTAAAACAAAAATGCAAACAGAAGAGGTGTGTGATGCCTCTGCTATTGTGGCAAAACATTCACAACCTAGTCCAGAGCCTCACAGTCCTACTGAACCTCCTGCATGGGGCAGCAGTATTGTGAAAGTTCCGTCTGGTATATTTGATGTCAACAGCAGGAAAAGTAGCACTGGTAGTATATCAAATGTGCTGTTTTCTACTCAAGATCCAGTTGAAGATGCAGTCTTTGGCGAAGCTACTAATCTCAAGAAGAATGGTGATAGAGGAGAAAAAAGACAAAAGCATTTTCCTGAGAGGAGTTGTAGTTTTAGTTCTGAAAGTCGAGCAGGAATGTTGCTTAAGAAGAGTAGTTTGGATTCGAATTCAAGTGAAATGGCTATCATGATGGGAGCAGATGCCAAGATTCTCACAGCAGCATTGACATGTCCTAAGACTTCTCTACTTCATATTGCAAGAACCCATAGCTTTGAGAATGTTAGCTGTCACCTACCTGATAGTAGGACTTGTATGTCTGAAAGCACTTGGAATCCTGAGCACAGATCATCTCCGGTGCCAGAGATGCTTGAGGAAAGCCAAGAACTCCTTGAGCCTGTGGTTGATGACGTACCTAAAACTACTGCAACAGTAGATACATATGAGAGTCTACTAAGTGATAGTAACAGTAATCAGTCCAGAGACTTGAAAACAGTATCCAAAGACCTGAGGAATAAGAGAAGTAGTTTATATGGTATTGCTAAGGTGGTTCAGAGGGAAGATGTTGAAACTGGACTAGATCCTTTGTCTCTTTTAGCCACTGAATGTACAGGAGGAAAAACTCCTGATTCTGAAGATAAGTTGTTTTCTCCAGTTATTGCACGTAATCTGGCTGATGAAATAGAAAGCTATATGAACCTAAAAAGTCCCCTAGGTAGTAAATCTTCTAGTATGGAATTACACAGAGAGGAAAACAGAGAGTCTGGCATGACTACTGCATTTATTCATGCTCTAGAGAGGAGATCAAGCCTACCTTTAGATCATGGTTCACCAGCACAGGAAAATCCTGAAAGTGAAAAGAGCTCACCTGCAGTGTCCAGGTCTAAAACTTTTACTGGGCGTTTCAAGCAGCAAACCCCCTCTCGAACTCATAAAGAACGTTCAACTTCTTTGTCAGCACTGGTGCGTTCTTCGCCACATGGCTCGTTGGGTTCTGTAGTAAATTCTTTGTCAGGGCTAAAGCTGGATAATATACTCTCAGGGCCCAAGATAGATGTCCTGAAATCTGGTATGAAACAAGCAGCGACAGTAGCCAGTAAGATGTGGGTAGCTGTTGCGTCTGCCTACAGCTACTCAGATGATGAGGAAGAAACTAATAGAGACTACAGCTTCCCAGCTGGCCTAGAAGACCATATTTTGGGGGAGAATATATCGCCTAACACAAGTATCTCAGGGTTGGTCCCCAGTGAACTTACCCAGAGCAACACAAGTCTTGGCAGTAGCAGCAGTAGTGGAGATGTAGGAAAACTGCATTATCCAACAGGTGAAGTTCCATTTCCAAGAGGCATGAAAGGGCAAGACTTTGAAAAATCAGATCATGGTTCTTCTCAAAATACCAGCATGTCTAGCATCTATCAGAATTGTGCAATGGAGGTTTTGATGTCCAGTTGTTCACAGTGTAGAGCTTGTGGAGCTTTAGTTTATGATGAAGAAATTATGGCTGGATGGACAGCAGATGACTCAAATTTGAATACAGCTTGTCCATTCTGTAAAAGCAACTTCTTGCCTCTTCTCAATATAGAATTCAAAGATTTGAGAGGTTCTGCAAGCTTTTTCCTGAAACCAAGTACCTCTGGTGACAGTTTACAAAGTGGAAGCATTCCATTGGCAAATGAATCCTTGGAGCACAAACCTGTATCCAGTTTAGCAGAACCTGACTTGATCAACTTTATGGACTTCCCAAAACATAACCAGATCATAACTGAAGAAACAGGCTCTGCAGTTGAACCAAGTGATGAAATAAAGAGAGCCAGTGGAGATGTCCAAACTATGAAAATTTCATCTGTGCCTAATAGTTTATCAAAGCGAAATGTGTCTTTGACTCGAAGTCACAGTGTTGGAGGCCCATTGCAGAATATTGACTTTACCCAGCGACCGTTTCATGGCATCTCAACAGTTAGTCTTCCAAATAGTCTGCAGGAAGTTGTGGATCCTTTAGGAAAAAGACCCAATCCTCCCCCTGTTTCTGTGCCCTACTTGAGTCCTCTAGTACTCCGTAAAGAACTTGAATCTTTGCTAGAAAATGAAGGTGATCAGGTGATTCATACATCTTCTTTCATCAATCAACATCCAATCATTTTCTGGAACCTCGTTTGGTATTTCAGACGTTTGGACCTTCCTAGTAACTTGCCAGGACTTATCCTCACATCTGAACATTGTAATGAAGGTGTACAGCTTCCTCTGTCATCTCTGTCCCAGGATAGCAAACTTGTGTATATTCAGCTGTTATGGGATAATATCAACCTTCATCAGGAACCAAGAGAACCTCTGTATGTCTCATGGAGGAATTTTAATTCTGAAAAGAAATCATCTCTCCTGTCAGAGGAACAACAAGAAACAAGCACTTTAGTAGAAACCATCAGGCAGAGTATTCAGCACAATAATGTTCTTAAACCCATCAACCTACTTTCACAGCAAATGAAGCCAGGCATGAAAAGACAAAGGAGTTTATACAGAGAAATCCTCTTCTTATCATTAGTGTCTCTAGGAAGAGAGAATATTGATATTGAGGCATTTGACAATGAATATGGAATTGCATACAATAGTCTGTCTTCAGAGATTCTTGAAAGGTTGCAGAAAATTGATGCTCCACCAAGTGCCAGTGTCGAGTGGTGCAGGAAGTGTTTTGGAGCGCCTCTCATTTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFASLEMIEDKGPRVTDYFVVAGLTDTSTLLDQEINRLDTKSTGPKAPITDIAI
IIKSAGETVPEGYTCVEATPSALQANLNYGSLKSPELFLCYKRGRDKPPLTDIGVLYEGKERLIPGCEVILATPYGRCANVNNSSTTSQRIFITYRRAPP
VRPQNSLAVTDICVIVTSKGETPPHTFCKVDKNLNCGMWGSSVFLCYKKSVPASNAIAYKAGLIFRYPEEDYESFPLSESDVPLFCLPMGATIECWDPET
KYPLPVFSTFVLTGSSAKKVYGAAIQFYEPYSRELLSEKQLMHLGLLTPVERKMVSKSINTNKCICLLSHWPFFEAFRKFLMFIYKLSVSGPHPLPIEKH
ISHFMQNIPFPSPQRPRILVQLSVHDALILSQPVSTPLPLSGANFSTLLMNLGPENCATLLLFVLLESKILLHSLRPAVLTGVAEAVVAMIFPFQWQCPY
IPLCPLSLAAVLSAPLPFIVGVDSRYFDLHDPPQDVVCIDLDTNMLYVSDEKKNMNWKQLPKKPCKNLLSTLKKLYPQLSSVHQKTQEGSAIDMTPIEAD
FSWQKKMTQLEMEIQEAFLRFMASILKGYRTYLRPITEAPSNKATAADSLFDRQGFLKSRDRAYAKFYTLLSKTQIFIRFIEECSFVSDKDTGLAFFDDC
IEKLFPDKGTEKTDKVDFDSAEDTRLIELDDSQKSEHTVFIMPPEPPPDDGKDLSPKYSYKYFPRLDLKLFDRPQELKLCFSRHPTGNSITKSPPLMAKR
TKQEIKTAHKLAKRCYTNPPQWAKCLFSHCYSLWFICLPAYVRVSHPKVRALQQAYDVLIKMRKTDVDPLDEVCYRVVMQLCGLWGHPVLAVRVLFEMKT
ARIKPNAITYGYYNKVVLESPWPSSTRSGIFLWTKVRNVVRGLAQFRQPLKKTVQRSQVSSISGGQSDQGYGSKDELIKDDAEIHVPEEQAARELITKTK
MQTEEVCDASAIVAKHSQPSPEPHSPTEPPAWGSSIVKVPSGIFDVNSRKSSTGSISNVLFSTQDPVEDAVFGEATNLKKNGDRGEKRQKHFPERSCSFS
SESRAGMLLKKSSLDSNSSEMAIMMGADAKILTAALTCPKTSLLHIARTHSFENVSCHLPDSRTCMSESTWNPEHRSSPVPEMLEESQELLEPVVDDVPK
TTATVDTYESLLSDSNSNQSRDLKTVSKDLRNKRSSLYGIAKVVQREDVETGLDPLSLLATECTGGKTPDSEDKLFSPVIARNLADEIESYMNLKSPLGS
KSSSMELHREENRESGMTTAFIHALERRSSLPLDHGSPAQENPESEKSSPAVSRSKTFTGRFKQQTPSRTHKERSTSLSALVRSSPHGSLGSVVNSLSGL
KLDNILSGPKIDVLKSGMKQAATVASKMWVAVASAYSYSDDEEETNRDYSFPAGLEDHILGENISPNTSISGLVPSELTQSNTSLGSSSSSGDVGKLHYP
TGEVPFPRGMKGQDFEKSDHGSSQNTSMSSIYQNCAMEVLMSSCSQCRACGALVYDEEIMAGWTADDSNLNTACPFCKSNFLPLLNIEFKDLRGSASFFL
KPSTSGDSLQSGSIPLANESLEHKPVSSLAEPDLINFMDFPKHNQIITEETGSAVEPSDEIKRASGDVQTMKISSVPNSLSKRNVSLTRSHSVGGPLQNI
DFTQRPFHGISTVSLPNSLQEVVDPLGKRPNPPPVSVPYLSPLVLRKELESLLENEGDQVIHTSSFINQHPIIFWNLVWYFRRLDLPSNLPGLILTSEHC
NEGVQLPLSSLSQDSKLVYIQLLWDNINLHQEPREPLYVSWRNFNSEKKSSLLSEEQQETSTLVETIRQSIQHNNVLKPINLLSQQMKPGMKRQRSLYRE
ILFLSLVSLGRENIDIEAFDNEYGIAYNSLSSEILERLQKIDAPPSASVEWCRKCFGAPLI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU63772
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No