cDNA Clone ULK1

• Synonyms: ATG1; ATG1A; hATG1; UNC51; Unc51.1
• Plasmid: pBabe.puro.GFP DU ULK1 S467A
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP ULK1 S467A
• Genbank: NM_003565.2 GI:225637564 with silent change c1065t and coding change c2141t (P714L) (exisiting SNP)
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCCTGGAGCCCGGCCGCGGCGGCACAGAGACCGTGGGCAAGTTCGA
GTTCTCCCGCAAGGACCTGATCGGCCACGGCGCCTTCGCGGTGGTCTTCA
AGGGCCGCCACCGCGAGAAGCACGATTTGGAGGTCGCCGTCAAGTGCATT
AACAAGAAGAACCTCGCCAAGTCTCAGACGCTGCTGGGGAAGGAAATCAA
AATCCTGAAGGAACTGAAACATGAAAACATCGTGGCCCTGTACGACTTCC
AGGAAATGGCTAATTCTGTCTACCTGGTTATGGAGTACTGCAACGGTGGG
GACCTGGCCGACTACCTGCACGCCATGCGCACGCTGAGCGAGGACACCAT
CAGGCTCTTCCTGCAGCAGATCGCGGGCGCCATGCGGCTTCTGCACAGCA
AAGGCATCATCCACCGCGACCTGAAACCGCAGAACATCCTGCTGTCCAAC
CCCGCCGGCCGCCGCGCCAACCCCAACAGCATCCGCGTCAAGATCGCTGA
CTTCGGCTTCGCGCGGTACCTCCAGAGCAACATGATGGCGGCCACACTCT
GCGGCTCCCCCATGTACATGGCCCCCGAGGTCATCATGTCCCAGCACTAC
GACGGGAAGGCGGACCTGTGGAGCATCGGCACCATCGTCTACCAGTGCCT
GACGGGGAAGGCGCCCTTCCAGGCCAGCAGCCCCCAGGACCTGCGCCTGT
TCTACGAGAAGAACAAGACGTTGGTCCCCACCATCCCCCGGGAGACCTCG
GCCCCGCTGCGGCAGCTGCTCCTGGCCCTACTGCAACGCAACCACAAGGA
CCGCATGGACTTCGATGAGTTTTTTCATCACCCTTTCCTCGATGCCAGCC
CCTCGGTCAGGAAATCCCCACCCGTGCCTGTGCCCTCGTACCCAAGCTCG
GGGTCCGGCAGCAGCTCCAGCAGCAGCTCCACCTCCCACCTGGCCTCCCC
GCCGTCCCTGGGCGAGATGCAGCAGCTGCAGAAGACCCTGGCCTCCCCGG
CTGACACCGCTGGCTTCCTGCACAGCTCCCGGGACTCTGGTGGCAGCAAG
GACTCTTCCTGTGACACAGATGACTTCGTCATGGTCCCCGCGCAGTTTCC
AGGTGACCTGGTGGCTGAGGCGCCCAGTGCCAAACCCCCGCCAGACAGCC
TGATGTGCAGTGGGAGCTCACTGGTGGCCTCTGCGGGCTTGGAGAGCCAC
GGCCGGACCCCATCTCCATCCCCACCCTGCAGCAGCTCCCCCAGTCCCTC
AGGCCGGGCTGGCCCGTTCTCCAGCAGCAGGTGCGGCGCCTCTGTCCCCA
TCCCAGTCCCCACGCAGGTGCAGAACTACCAGCGCATTGAGCGAAACCTG
CAGTCACCCACCCAGTTCCAAACACCTCGGTCCTCTGCCATCCGCAGGTC
AGGCgcCACCAGCCCCCTGGGCTTTGCAAGGGCCAGCCCCTCGCCCCCTG
CCCACGCTGAGCATGGAGGCGTCCTGGCCAGGAAGATGTCTCTGGGTGGA
GGCCGGCCCTACACGCCATCTCCTCAAGTTGGAACCATCCCTGAGCGGCC
AGGCTGGAGCGGGACGCCCTCCCCACAGGGAGCTGAGATGCGGGGTGGCA
GGTCCCCTCGTCCAGGCTCCTCTGCACCCGAGCACTCTCCCCGCACTTCC
GGGCTGGGCTGCCGCCTGCACAGCGCCCCCAACCTGTCTGACTTGCACGT
CGTCCGCCCCAAGCTGCCCAAACCCCCCACGGACCCCCTGGGAGCTGTGT
TCAGCCCACCACAGGCCAGCCCTCCCCAGCCGTCCCACGGCCTGCAGTCC
TGCCGGAACCTGCGGGGCTCACCCAAGCTGCCCGACTTCCTGCAGCGAAA
CCCCCTGCCCCCCATCCTGGGCTCCCCCACCAAGGCTGTGCCCTCCTTTG
ACTTCCCGAAGACCCCCAGCTCCCAGAACCTGCTGGCCCTCCTAGCCCGG
CAGGGCGTGGTGATGACGCCCCCTCGAAACCGGACGCTGCCCGACCTCTC
GGAGGTGGGACCCTTCCATGGTCAGCCGTTGGGCCCTGGCCTGCGGCCAG
GCGAGGACCCCAAGGGCCCCTTTGGCCGGTCTTTCAGCACCAGCCGCCTC
ACTGACCTGCTCCTTAAGGCGGCGTTTGGGACACAAGCCCCGGACCTGGG
CAGCACGGAGAGCCTGCAGGAGAAGCCCATGGAGATCGCACCCTCAGCTG
GCTTTGGAGGGAGCCTGCACCCAGGAGCCCGTGCTGGGGGCACCAGCAGC
CCTTCCCCGGTGGTCTTCACCGTGGGCTCTCCCCCGAGCGGGAGCACGCC
CCCCCAGGGCCCCCGCACCAGGATGTTCTCAGCGGGCCCCACTGGCTCTG
CCAGCTCTTCTGCCCGCCACCTGGTGCCTGGGCCCTGCAGCGAGGCCCCA
GCCCCTGAGCTCCCTGCTCCAGGACACGGCTGCAGCTTTGCCGACCCCAT
TGCTGCGAACCTGGAGGGGGCTGTGACCTTCGAGGCCCCCGACCTCCCTG
AGGAGACCCTCATGGAGCAAGAGCACACGGAGATCCTGCGTGGCCTGCGC
TTCACGCTGCTGTTCGTGCAGCACGTCCTGGAGATCGCAGCCCTGAAGGG
CAGCGCCAGTGAGGCGGCGGGGGGCCCTGAGTACCAGCTGCAGGAGAGTG
TGGTGGCCGACCAGATCAGCCTGCTGAGCCGAGAATGGGGCTTCGCGGAA
CAGCTGGTGCTGTACCTGAAGGTGGCCGAGCTACTGTCCTCCGGCCTGCA
AAGTGCCATCGACCAGATCCGGGCCGGCAAGCTCTGCCTGTCGTCCACTG
TGAAGCAGGTGGTGCGCAGGCTGAATGAGCTGTACAAGGCCAGCGTGGTG
TCCTGCCAGGGCCTGAGCCTGCGGCTGCAGCGCTTCTTCCTGGACAAGCA
GCGGCTCCTGGACCGCATTCACAGCATCACTGCCGAGAGGCTCATCTTCA
GCCACGCTGTGCAGATGGTGCAGTCGGCTGCCCTGGACGAGATGTTCCAG
CACCGTGAGGGCTGCGTCCCACGCTACCACAAGGCCCTGCTGCTCCTGGA
GGGGCTGCAGCACATGCTCTCGGACCAGGCCGACATCGAGAACGTCACCA
AGTGCAAGCTGTGCATTGAGCGGAGACTCTCGGCGCTGCTGACTGGCATC
TGTGCCTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSLEPGRG
GTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKS
QTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHA
MRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANP
NSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWS
IGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLL
ALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSS
SSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSSCDTDD
FVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSP
PCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQT
PRSSAIRRSGATSPLGFARASPSPPAHAEHGGVLARKMSLGGGRPYTPSP
QVGTIPERPGWSGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLGCRLHS
APNLSDLHVVRPKLPKPPTDPLGAVFSPPQASPPQPSHGLQSCRNLRGSP
KLPDFLQRNPLPPILGSPTKAVPSFDFPKTPSSQNLLALLARQGVVMTPP
RNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPFGRSFSTSRLTDLLLKAA
FGTQAPDLGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSSPSPVVFTV
GSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAPAPELPAPG
HGCSFADPIAANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTLLFVQH
VLEIAALKGSASEAAGGPEYQLQESVVADQISLLSREWGFAEQLVLYLKV
AELLSSGLQSAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLR
LQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQSAALDEMFQHREGCVPR
YHKALLLLEGLQHMLSDQADIENVTKCKLCIERRLSALLTGICA*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pBabe.puro.GFP DU ULK1 S467A56.86 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65166
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N.B. Grow at 30 degrees.; silent change c1065t and coding change c2141t (P714L) (exisiting SNP) Grow at or below 30ºC
• Low Temp: Yes