cDNA Clone TRAIP

• Synonyms: TRIP
• Plasmid: pRF100 mouseTRAIP 5FLAG Knockin
• Expressed: TRAIP internal 5FLAG tag
• Genbank: NP_035764.2
• Species: Mouse

Amino Acid Sequence

MPIRALCTICSDFFDHSRDVAAIHCGHTFHLQCLIQWFETAPSRTCPQCRIQVGKKTIINKLFFDLAQEEENVLDAEFLKNELDSVKAQLSQKDREKRDSQAIIDTLRDTLEERNATVESLQNALNKAEMLCSTLKKQMKFLEQRQDETKQAREEAHRLKCKMKTMEQIELLLQSQRSEVEEMIRDMGVGQSAVEQLAVYCVSLKKEYENLKEARKATGELADRLKKDLVSSRSKLKTLNTELDQAKLELRSAQKDLQSADQEITSLRKKLMILQGTLSLPPATNETVSRLVFESPAPVEMMNPRLHQPPFGDEIDLNTTFDVNTPPTQTSGSQHCGAGAGAGADYKDDDDKDYKDDDDKLMDYKDDDDKDYKDDDDKLMDYKDDDDKGAGAGAGALPKKLCLERARSPMQNVLKKVHKVSKPESQLSLGGQRCVGELDEELAGAFPLFIRNAVLGQKQPNRTTAESRCSTDVVRIGFDGLGGRTKFIQPRDTTIIRPVPVKSKAKSKQKVRIKTVSSASQPKLDTFLCQ

• Vector Type: Mammalian
• Full Snapgene Map: pRF100 mouseTRAIP 5FLAG Knockin113.15 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU78217
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Deposited by Ryo Fujisawa (Karim Labib group MRCPPU). cloned by Gibson assembly; ; TRAIP with internal 5FLAG tag (C336 and L337) expression cassette with CAG promoter to integrate at ROSA26 locus; ; 2610-4420 Mouse TRAIP ∆169-206 coding sequence19-888 ROSA26 5' arm889-2584 CAG promoter4488-5741 ROSA26 3' armAmpR
• Low Temp: No