cDNA Clone UBCH6

• Synonyms: UBE2E1
• Plasmid: pET156P UBCH6
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His UBCH6
• Genbank: AAH09139
• Tag: 6His
• Species: Human

Sequence of Insert

GCGGCCGCGATGTCGGATGACGATTCGAGGGCCAGCACCAGCTCCTCCTC
ATCTTCGTCTTCCAACCAGCAAACCGAGAAAGAAACAAACACCCCCAAGA
AGAAGGAGAGTAAAGTCAGCATGAGCAAAAACTCCAAACTCCTCTCCACC
AGCGCCAAGAGAATTCAGAAGGAGCTGGCGGACATCACTTTAGACCCTCC
ACCTAATTGCAGTGCTGGTCCCAAAGGCGATAACATCTATGAATGGAGAT
CAACCATTCTAGGGCCTCCAGGATCCGTGTATGAGGGTGGTGTATTCTTT
CTCGATATCACTTTTACACCAGAATATCCCTTCAAGCCTCCAAAGGTTAC
ATTTCGGACAAGAATCTATCATTGTAATATTAACAGTCAAGGTGTTATTT
GCTTGGACATATTGAAAGATAATTGGAGTCCAGCACTAACCATTTCTAAA
GTCCTCCTTTCTATCTGCTCACTTCTTACAGACTGTAATCCTGCCGACCC
CTTGGTGGGAAGTATTGCCACTCAGTATATGACCAACAGAGCAGAACATG
ACAGAATGGCCAGACAGTGGACCAAGAGATACGCTACATAAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSPEFPGVDSKAAAMSDDDSRASTSSSSS
SSSNQQTEKETNTPKKKESKVSMSKNSKLLSTSAKRIQKELADITLDPPP
NCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTF
RTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPL
VGSIATQYMTNRAEHDRMARQWTKRYAT*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET156P UBCH646.15 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU12803
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6HIS for bacterial expression.; ; Estimated MW of expressed protein : 25018 Daltons
• Low Temp: No