cDNA Clone USP27X

• Synonyms: MRX105; USP22L; USP27
• Plasmid: pGEX6P1 USP27X
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C USP27X
• Genbank: NM_001145073.3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGTGTAAGGACTATGTATATGACAAAGACATTGAGCAAATTGCCAAAGAAGAGCAAGGAGAAGCTTTGAAATTACAAGCCTCCACCTCAACAGAGGTTTCTCACCAGCAGTGTTCAGTGCCAGGCCTTGGTGAGAAATTCCCAACCTGGGAAACAACCAAACCAGAATTAGAACTGCTGGGGCACAACCCGAGGAGAAGAAGAATCACCTCCAGCTTTACGATCGGTTTAAGAGGACTCATCAATCTTGGCAACACGTGCTTTATGAACTGCATTGTCCAGGCCCTCACCCACACGCCGATACTGAGAGATTTCTTTCTCTCTGACAGGCACCGATGTGAGATGCCGAGTCCCGAGTTGTGTCTGGTCTGTGAGATGTCGTCGCTGTTTCGGGAGTTGTATTCTGGAAACCCGTCTCCTCATGTGCCCTATAAGTTACTGCACCTGGTGTGGATACATGCCCGCCATTTAGCAGGGTACAGGCAACAGGATGCCCACGAGTTCCTCATTGCAGCGTTAGATGTCCTGCACAGGCACTGCAAAGGTGATGATGTCGGGAAGGCGGCCAACAATCCCAACCACTGTAACTGCATCATAGACCAAATCTTCACAGGTGGCCTGCAGTCTGATGTCACCTGTCAAGCCTGCCATGGCGTCTCCACCACGATAGACCCATGCTGGGACATTAGTTTGGACTTGCCTGGCTCTTGCACCTCCTTCTGGCCCATGAGCCCAGGGAGGGAGAGCAGTGTGAACGGGGAAAGCCACATACCAGGAATCACCACCCTCACGGACTGCTTGCGGAGGTTTACGAGGCCAGAGCACTTAGGAAGCAGTGCCAAAATCAAATGTGGTAGTTGCCAAAGCTACCAGGAATCTACCAAACAGCTCACAATGAATAAATTACCTGTCGTTGCCTGTTTTCATTTCAAACGGTTTGAACATTCAGCGAAACAGAGGCGCAAGATCACTACATACATTTCCTTTCCTCTGGAGCTGGATATGACGCCGTTTATGGCCTCAAGTAAAGAGAGCAGAATGAATGGACAATTGCAGCTGCCAACCAATAGTGGAAACAACGAAAATAAGTATTCCTTGTTTGCTGTGGTTAATCACCAAGGAACCTTGGAGAGTGGCCACTATACCAGCTTCATCCGGCACCACAAGGACCAGTGGTTCAAGTGTGATGATGCCGTCATCACTAAGGCCAGTATTAAGGACGTACTGGACAGTGAAGGGTATTTACTGTTCTATCACAAACAGGTGCTAGAACATGAGTCAGAAAAAGTGAAAGAAATGAACACACAAGCCTACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMCKDYVYDKDIEQIAKEEQ
GEALKLQASTSTEVSHQQCSVPGLGEKFPTWETTKPELELLGHNPRRRRI
TSSFTIGLRGLINLGNTCFMNCIVQALTHTPILRDFFLSDRHRCEMPSPE
LCLVCEMSSLFRELYSGNPSPHVPYKLLHLVWIHARHLAGYRQQDAHEFL
IAALDVLHRHCKGDDVGKAANNPNHCNCIIDQIFTGGLQSDVTCQACHGV
STTIDPCWDISLDLPGSCTSFWPMSPGRESSVNGESHIPGITTLTDCLRR
FTRPEHLGSSAKIKCGSCQSYQESTKQLTMNKLPVVACFHFKRFEHSAKQ
RRKITTYISFPLELDMTPFMASSKESRMNGQLQLPTNSGNNENKYSLFAV
VNHQGTLESGHYTSFIRHHKDQWFKCDDAVITKASIKDVLDSEGYLLFYH
KQVLEHESEKVKEMNTQAY

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 USP27X74.6 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU21193
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GST vector for bacterial expression; ; Estimated MW of expressed protein : 76460 Daltons
• Low Temp: No