cDNA Clone Ubiquitin

• Plasmid: pET24a-ubiquitin (c220a) K11R
• Parent Plasmid: pET24a original
• Expressed: untagged Ubiquitin [K11R]
• Genbank: P62988

Sequence of Insert

CATATGCAGATCTTCGTGAAGACCCTGACTGGTAGGACCATCACTCTCGA
AGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGACA
AGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAG
CTGGAAGATGGACGCACCCTGTCTGACTACAACATCCAGAAAGAGTCCAC
CCTGCACCTGGTCCTCCGTCTCCGAGGTGGGTGATAAGGATCC

Amino Acid Sequence

MQIFVKTLTGRTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQL
EDGRTLSDYNIQKESTLHLVLRLRGG**

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU23831
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Arg73 codon AGA mutated to more common CGA