cDNA Clone RNF1-233S

• Plasmid: pET15 6HIS SUMO RNF1-233s
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6His SUMO RNF1-233s
• Genbank: synthetic gene
• Tag: 6His
• Tag: SUMO

Sequence of Insert

GGATCCATGACCACCACCCGTTATCGTCCGACCTGGGATCTGGCACTGGACCCGCTGGTTAGCTGTAAACTGTGTCTGGGTGAATATCCGGTTGAGCAGATGACCACAATTGCACAGTGTCAGTGTATTTTTTGTACCCTGTGTCTGAAACAGTATGTGGAACTGCTGATTAAAGAAGGTCTGGAAACCGCAATTAGCTGTCCGGATGCAGCATGTCCGAAACAGGGCCATCTGCAAGAAAATGAAATTGAATGTATGGTGGCAGCCGAAATTATGCAGCGTTACAAAAAACTGCAATTTGAACGCGAAGTTCTGTTTGATCCGTGTCGTACCTGGTGTCCGGCAAGCACCTGTCAGGCAGTTTGTCAGCTGCAGGATGTTGGCCTGCAGACACCGCAGCCGGTTCAGTGTAAAGCATGTCGTATGGAATTTTGCAGCACCTGTAAAGCAAGCTGGCATCCGGGTCAGGGTTGTCCGGAAACCATGCCGATTACCTTTCTGCCTGGTGAAACCAGCGCAGCCTTTAAAATGGAAGAGGATGATGCACCGATTAAACGTTGTCCGAAATGCAAAGTGTATATCGAACGTGATGAAGGTTGTGCACAGATGATGTGCAAAAATTGCAAACATGCCTTTTGCTGGTATTGTCTGGAAAGCCTGGATGATGATTTTCTGCTGATCCACTATGATAAAGGTCCGTAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMTTTRYRPTWDLALDPLVSCKLCLGEYPVEQMTTIAQCQCIFCTLCLKQYVELLIKEGLETAISCPDAACPKQGHLQENEIECMVAAEIMQRYKKLQFEREVLFDPCRTWCPASTCQAVCQLQDVGLQTPQPVQCKACRMEFCSTCKASWHPGQGCPETMPITFLPGETSAAFKMEEDDAPIKRCPKCKVYIERDEGCAQMMCKNCKHAFCWYCLESLDDDFLLIHYDKGP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU22728
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: linker sequence after Senp cleavage deleted; Parental Plasmid DU32993