cDNA Clone RNF144A

• Plasmid: pET15 6HIS SUMO RNF144a M1-R233 C214A
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6His-SUM-RNF144 M1-R233 C214A
• Genbank: NM_014746.3
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

ATGGGTCATCATCACCATCACCATTCTGACCAGGAGGCAAAACCTTCAAC
TGAGGACTTGGGGGATAAGAAGGAAGGTGAATATATTAAACTCAAAGTCA
TTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATGACAACACAT
CTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATGAA
TTCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATACTC
CAAAAGAACTGGGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGGAA
CAAACGGGG
ggaATGACCACAACAAGGTACCGGCCCACCTGGGACCTGGCCCTCGACCCGCTGGTGTCTTGCAAGCTCTGTCTTGGGGAGTACCCAGTGGAGCAGATGACAACCATAGCCCAGTGCCAATGCATCTTCTGTACTCTGTGCCTGAAACAGTATGTTGAGCTCTTGATCAAAGAAGGATTAGAAACCGCAATTAGCTGCCCAGATGCTGCCTGCCCTAAACAGGGCCACCTACAGGAGAACGAGATTGAGTGCATGGTTGCAGCTGAAATTATGCAAAGATATAAAAAGCTACAATTTGAAAGAGAGGTGCTGTTTGATCCCTGTCGGACTTGGTGCCCGGCGTCCACCTGCCAAGCTGTGTGTCAGCTCCAGGACGTGGGGCTGCAGACCCCCCAGCCAGTGCAGTGCAAAGCCTGCCGTATGGAATTCTGCTCCACCTGCAAAGCCAGCTGGCACCCTGGCCAGGGCTGCCCGGAGACCATGCCGATCACCTTCCTCCCCGGGGAGACCAGTGCTGCTTTCAAAATGGAAGAAGATGACGCGCCCATCAAGCGCTGCCCCAAGTGCAAAGTCTACATCGAGCGAGACGAAGGCTGCGCGCAGATGATGTGCAAGAACTGCAAGCACGCCTTCTGTTGGTACGCCCTGGAGTCTCTGGACGATGATTTCCTTCTGATACACTACGATAAGGGACCCTGCCGGTAGgcggccgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMTTTRYRPTWDLALDPLVSCKLCLGEYPVEQMTTIAQCQCIFCTLCLKQYVELLIKEGLETAISCPDAACPKQGHLQENEIECMVAAEIMQRYKKLQFEREVLFDPCRTWCPASTCQAVCQLQDVGLQTPQPVQCKACRMEFCSTCKASWHPGQGCPETMPITFLPGETSAAFKMEEDDAPIKRCPKCKVYIERDEGCAQMMCKNCKHAFCWYALESLDDDFLLIHYDKGPCR*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU22776
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Linker sequence after senp cleavage deleted; Parental Plasmid DU32993