cDNA Clone TRAK2

• Synonyms: ALS2CR3, CALS-C, GRIF-1, GRIF1, MILT2, OIP98
• Plasmid: pSCB TRAK2 N659S Bam Not
• Parent Plasmid: pSC-B
• Genbank: NM_015049.2
• Species: Human

Sequence of Insert

GGATCCATGAGTCAATCCCAGAATGCAATTTTTACATCACCAACAGGTGAAGAAAACCTCATGAATAGCAATCACAGAGACTCGGAGAGCATCACTGATGTCTGCTCCAATGAGGATCTCCCTGAAGTTGAGCTGGTGAGTCTGCTAGAAGAACAACTACCACAGTATAGGCTAAAAGTAGACACTCTCTTTCTATATGAAAATCAAGACTGGACTCAGTCTCCACACCAGCGGCAGCATGCATCTGATGCTCTCTCTCCAGTCCTTGCTGAAGAGACTTTCCGTTACATGATTCTAGGCACAGACAGGGTGGAGCAGATGACCAAAACTTACAATGACATCGACATGGTTACACATCTCCTGGCAGAGAGGGATCGTGATCTGGAACTCGCTGCTCGAATTGGACAAGCTCTCTTAAAGCGGAACCATATCTTATCTGAGCAGAACGAATCCCTGGAGGAGCAATTGGGACAAGCCTTTGATCAAGTTAATCAGCTGCAGCATGAGCTATGCAAGAAAGATGAGTTACTTCGAATCGTCTCCATTGCTTCTGAAGAAAGTGAAACTGATTCCAGCTGTTCTACACCTCTTCGGTTCAATGAGTCCTTTAGCTTATCTCAAGGGTTGCTGCAGTTGGAAATGCTGCAAGAAAAGCTCAAGGAACTGGAAGAAGAGAATATGGCTCTTCGATCCAAGGCTTGTCACATAAAGACAGAAACTGTTACCTATGAAGAAAAGGAACAACAGCTTGTCAGCGACTGTGTTAAAGAACTTCGTGAAACAAATGCTCAGATGTCCAGAATGACTGAAGAATTGTCAGGGAAGAGTGATGAGCTGGTTCGATACCAAGAAGAGCTTTCCTCTCTTTTGTCACAGATTGTAGACCTTCAGCATAAACTTAAAGAACATGTGATTGAGAAGGAAGAACTAAAACTTCACCTGCAAGCTTCCAAAGATGCCCAACGGCAACTGACAATGGAGCTGCACGAGTTACAAGACAGGAATATGGAGTGTCTAGGAATGTTACATGAATCCCAAGAAGAAATAAAGGAACTTCGTAGTAGATCTGGCCCTACTGCTCATCTCTACTTCTCCCAATCATATGGAGCTTTTACTGGGGAATCTTTGGCAGCTGAGATTGAGGGGACTATGCGTAAAAAGCTGAGTTTGGATGAGGAATCTTCTCTCTTTAAACAAAAAGCCCAACAGAAGCGGGTATTTGATACCGTCAGGATTGCCAATGACACACGGGGCCGCTCTATCTCATTCCCAGCTCTGTTACCCATTCCAGGCTCCAACCGTTCAAGTGTCATCATGACAGCAAAACCTTTTGAGTCTGGTCTTCAGCAAACAGAGGACAAATCACTCCTGAACCAGGGGAGCAGCTCAGAGGAGGTTGCAGGGAGCTCCCAGAAGATGGGCCAACCAGGACCCTCAGGAGATAGTGATTTGGCTACAGCACTGCATCGCCTTAGCTTGCGTCGACAAAACTATTTAAGTGAGAAGCAGTTCTTTGCTGAAGAATGGCAGCGGAAGATCCAGGTTCTGGCAGACCAGAAGGAAGGAGTTAGTGGCTGTGTCACCCCGACAGAGAGCCTTGCCTCTCTCTGCACCACCCAGTCAGAGATCACAGACCTCAGCAGTGCCAGTTGCCTTCGAGGTTTTATGCCAGAAAAATTACAAATTGTCAAGCCCCTTGAAGGATCACAAACTCTGTATCACTGGCAGCAGCTTGCTCAACCAAACTTGGGAACCATCCTTGATCCACGACCAGGTGTCATTACTAAAGGCTTTACCCAGTTGCCCGGGGATGCTATTTATCACATCTCAGATTTAGAAGAGGATGAAGAGGAGGGTATTACTTTTCAGGTTCAGCAACCTCTTGAAGTGGAAGAGAAACTTTCAACATCCAAGCCAGTAACAGGGATCTTCCTGCCACCCATTACTTCAGCAGGTGGACCAGTTACAGTTGCAACCGCCAGCCCAGGAAAGTGCCTGTCGTGCACAAACTCAACATTCACTTTCACCACCTGTAGAATATTACATCCCTCTGACATCACTCAGGTTACCCCCAGCTCTGGGTTCCCTTCATTATCCTGTGGAAGTAGCGGTAGCAGTTCATCCAACACGGCTGTGAATTCTCCTGCCTTGTCCTATAGACTCAGCATTGGTGAGTCCATCACCAACCGACGAGATTCCACTACAACCTTCAGTAGCACCATGAGCTTGGCCAAACTTCTACAAGAGCGAGGCATCTCTGCCAAAGTGTACCACAGCCCAATTTCAGAGAACCCCCTCCAGCCTCTCCCTAAATCCCTGGCTATCCCTTCCACACCACCAAATTCACCATCTCACTCACCTTGCCCTTCTCCTTTACCCTTTGAGCCTCGAGTGCATCTCTCTGAAAATTTTTTGGCCTCTCGACCAGCTGAGACATTCCTCCAGGAGATGTATGGCTTGAGACCCTCCCGGAACCCTCCTGATGTTGGCCAGTTGAAGATGAACTTAGTGGACAGGCTGAAGAGACTGGGGATAGCCAGAGTGGTCAAGAACCCTGGTGCCCAAGAGAATGGAAGATGCCAGGAGGCAGAAATTGGTCCTCAAAAACCAGATTCTGCTGTTTATTTAAATTCAGGTAGCAGTTTATTAGGTGGACTAAGGAGGAATCAGAGTCTTCCAGTCATAATGGGTAGCTTTGCTGCCCCAGTTTGCACATCCTCACCCAAAATGGGTGTCCTGAAGGAGGACTGAGCGGCCGC

Amino Acid Sequence

MSQSQNAIFTSPTGEENLMNSNHRDSESITDVCSNEDLPEVELVSLLEEQ
LPQYRLKVDTLFLYENQDWTQSPHQRQHASDALSPVLAEETFRYMILGTD
RVEQMTKTYNDIDMVTHLLAERDRDLELAARIGQALLKRNHILSEQNESL
EEQLGQAFDQVNQLQHELCKKDELLRIVSIASEESETDSSCSTPLRFNES
FSLSQGLLQLEMLQEKLKELEEENMALRSKACHIKTETVTYEEKEQQLVS
DCVKELRETNAQMSRMTEELSGKSDELVRYQEELSSLLSQIVDLQHKLKE
HVIEKEELKLHLQASKDAQRQLTMELHELQDRNMECLGMLHESQEEIKEL
RSRSGPTAHLYFSQSYGAFTGESLAAEIEGTMRKKLSLDEESSLFKQKAQ
QKRVFDTVRIANDTRGRSISFPALLPIPGSNRSSVIMTAKPFESGLQQTE
DKSLLNQGSSSEEVAGSSQKMGQPGPSGDSDLATALHRLSLRRQNYLSEK
QFFAEEWQRKIQVLADQKEGVSGCVTPTESLASLCTTQSEITDLSSASCL
RGFMPEKLQIVKPLEGSQTLYHWQQLAQPNLGTILDPRPGVITKGFTQLP
GDAIYHISDLEEDEEEGITFQVQQPLEVEEKLSTSKPVTGIFLPPITSAG
GPVTVATASPGKCLSCTNSTFTFTTCRILHPSDITQVTPSSGFPSLSCGS
SGSSSSNTAVNSPALSYRLSIGESITNRRDSTTTFSSTMSLAKLLQERGI
SAKVYHSPISENPLQPLPKSLAIPSTPPNSPSHSPCPSPLPFEPRVHLSE
NFLASRPAETFLQEMYGLRPSRNPPDVGQLKMNLVDRLKRLGIARVVKNP
GAQENGRCQEAEIGPQKPDSAVYLNSGSSLLGGLRRNQSLPVIMGSFAAP
VCTSSPKMGVLKED*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU44465
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Contains an N659S error which requires repair. Two known residue variations V142I & I278V are also present which are benign according to ensembl and will be left in situ.