cDNA Clone ZUFSP

• Plasmid: pGEX-C3-HALO_Thr-ZUFSP
• Parent Plasmid: pGEX6P Halo Thr
• Expressed: GST-HALO-ZUFSP
• Genbank: NP_659499 NM_145062
• Tag: GST
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCatgctttcctgtaatatttgtggtgaaacagtaacctcagaacc
agacatgaaagctcacctaattgttcacatggaaagtgaaattatatgtc
cattttgcaagttgtcaggtgtgaattatgatgaaatgtgttttcatatc
gaaacagctcattttgagcagaatacacttgaaagaaactttgagaggat
aaatacagtacaatatggaacttcagataacaagaaagacaacaccctac
agtgtggaatggaagttaattcaagtattctttcaggttgtgcatctaat
catccaaaaaattcagctcaaaacctgactaaagatagtactttaaaaca
tgaaggcttctattcagagaacttaactgaatctagaaaattcctgaaaa
gtagggaaaaacagtccagcctgaccgaaataaaaggatctgtttatgaa
acaacatacagtcctcctgaatgtccattctgtggaaaaatagaggagca
cagtgaagatatggaaactcatgtgaaaacaaagcatgccaatcttttag
acattccattggaagactgtgatcaaccactctatgattgtcctatgtgt
gggctcatatgtacaaattaccatattcttcaggaacatgttgacttgca
tttggaagaaaacagctttcagcaaggcatggatagagtccagtgttctg
gtgatctacaattggctcaccagcttcagcaagaagaagacagaaagagg
agatctgaagaatcaagacaagaaatagaagaatttcagaagctgcagag
acaatatggtttagataattctggaggatacaaacaacaacaactacgaa
atatggagatagaagtaaataggggaagaatgcctccatctgaatttcat
aggagaaaagctgatatgatggaatcattagctcttggttttgacgatgg
aaaaacaaaaacttccggaattattgaagcacttcataggtattatcaga
atgctgccacagatgtgagacgggtgtggctttcttcagtggtggatcac
tttcattcatctttaggcgacaaaggttggggttgtggttacagaaattt
ccaaatgctactttcatcattattacaaaatgatgcttacaacgattgct
taaaaggtatgttgattccttgcattccaaaaattcaatctatgattgaa
gatgcatggaaggaaggttttgatcctcagggggcctctcaacttaataa
caggttacagggaacaaaggcctggattggagcatgtgaagtatatatac
tcctgacctccctaagggtaaagtgtcatattgttgattttcacaaatca
actggtcctttgggtacacaccctcgcttatttgaatggatattgaacta
ttattcttcagagggagaagggagtccaaaggtagtgtgtacatctaaac
ctcctatctatcttcagcatcaaggtcacagtcgaactgttattggaatt
gaagagaaaaaaaaccgaacattatgcttactaatacttgatcctggatg
tccttctcgagaaatgcagaaattattaaagcaagacatagaggctagca
gtctcaagcaacttcggaaatctatgggaaatttaaaacataagcaatac
cagatattggcagtagagggtgctctttctctagaggagaaacttgccag
gagacaagcttctcaagtctttacagccgagaagattccttgaGCGGCCG
C

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAEIGTGFPFDPHYVEVLG
ERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGM
GKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRN
PERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFI
EGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIV
ALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGP
GLNLLQEDNPDLIGSEIARWLSTLEISGLVPRGSGSMLSCNICGETVTSE
PDMKAHLIVHMESEIICPFCKLSGVNYDEMCFHIETAHFEQNTLERNFER
INTVQYGTSDNKKDNTLQCGMEVNSSILSGCASNHPKNSAQNLTKDSTLK
HEGFYSENLTESRKFLKSREKQSSLTEIKGSVYETTYSPPECPFCGKIEE
HSEDMETHVKTKHANLLDIPLEDCDQPLYDCPMCGLICTNYHILQEHVDL
HLEENSFQQGMDRVQCSGDLQLAHQLQQEEDRKRRSEESRQEIEEFQKLQ
RQYGLDNSGGYKQQQLRNMEIEVNRGRMPPSEFHRRKADMMESLALGFDD
GKTKTSGIIEALHRYYQNAATDVRRVWLSSVVDHFHSSLGDKGWGCGYRN
FQMLLSSLLQNDAYNDCLKGMLIPCIPKIQSMIEDAWKEGFDPQGASQLN
NRLQGTKAWIGACEVYILLTSLRVKCHIVDFHKSTGPLGTHPRLFEWILN
YYSSEGEGSPKVVCTSKPPIYLQHQGHSRTVIGIEEKKNRTLCLLILDPG
CPSREMQKLLKQDIEASSLKQLRKSMGNLKHKQYQILAVEGALSLEEKLA
RRQASQVFTAEKIP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24210
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GST-C3-HALO-Thrombin fusion; Parental: 24192