cDNA Clone RNF144B

• Synonyms: E3 ubiquitin-protein ligase RNF144B
• Plasmid: pET15 6HIS SUMO RNF144B M1-M252
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: 6His SUMO-RNF144B M1-M252
• Genbank: synthesized gene, NM_182757.3
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

ggatccATGGGCTCCGCTGGCCGTCTGCATTATCTGGCGATGACCGCTGAAAACCCGACCCCGGGCGACCTGGCACCGGCACCGCTGATTACGTGCAAACTGTGCCTGTGTGAACAGAGCCTGGATAAAATGACCACGCTGCAGGAATGCCAATGTATCTTTTGCACCGCATGTCTGAAACAGTATATGCAACTGGCTATTCGCGAAGGCTGCGGTTCCCCGATCACCTGCCCGGATATGGTGTGTCTGAACCATGGCACGCTGCAAGAAGCAGAAATCGCTTGTCTGGTCCCGGTGGACCAGTTTCAACTGTATCAGCGTCTGAAATTCGAACGCGAAGTCCACCTGGACCCGTACCGTACCTGGTGTCCGGTTGCGGACTGCCAGACGGTGTGTCCGGTTGCCAGCTCTGATCCGGGTCAACCGGTTCTGGTCGAATGCCCGAGCTGTCATCTGAAATTTTGCTCTTGCTGTAAAGATGCGTGGCACGCCGAAGTGTCATGTCGCGACTCGCAGCCGATTGTTCTGCCGACCGAACATCGTGCACTGTTCGGTACGGACGCAGAAGCACCGATTAAACAGTGCCCGGTCTGTCGTGTGTACATCGAACGCAACGAAGGTTGCGCCCAAATGATGTGCAAAAATTGTAAACACACCTTTTGCTGGTATTGTCTGCAGAACCTGGATAATGACATTTTCCTGCGCCATTACGATAAAGGCCCGTGTCGTAACAAACTGGGTCACAGTCGCGCGTCCGTTATGTGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGSMGSAGRLHYLAMTAENPTPGDLAPAPLITCKLCLCEQSLDKMTTLQECQCIFCTACLKQYMQLAIREGCGSPITCPDMVCLNHGTLQEAEIACLVPVDQFQLYQRLKFEREVHLDPYRTWCPVADCQTVCPVASSDPGQPVLVECPSCHLKFCSCCKDAWHAEVSCRDSQPIVLPTEHRALFGTDAEAPIKQCPVCRVYIERNEGCAQMMCKNCKHTFCWYCLQNLDNDIFLRHYDKGPCRNKLGHSRASVM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU25314
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Needs mutated to create no overhang after SENP digestion. ; Parental Plasmid DU32993