cDNA Clone RNF144-HOIP chimera

• Plasmid: pGEX TEV RNF144 (L17-S64) - HOIP (C744-K1072)
• Parent Plasmid: pGEX TEV
• Expressed: GST-TEV-RNF144 (L17-S64) - HOIP (C744-K1072)
• Genbank: NM_017999.4 (HOIP) and NM_014746.3 (RNF144)
• Tag: GST
• Species: Human

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDENLYFQGGSPEFLVSCKLCLGEYPVEQMTT
IAQCQCIFCTLCLKQYVELLIKEGLETAISCPACGRPDLTDDTQLLSYFS
TLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQL
EATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRMNDPEYQAQGLAMY
LQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPE
PNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARA
VPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAH
SLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPL
GQSIPRRRK*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU24298
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: (DU20757) parental. Two rounds of insertion mutagenesis with NW2680/81 and NW2682/83 to replace HOIP 697-743 (QEC....TDMV) in DU22629 with RNF144A 17-64 (LVS....AIS)