cDNA Clone SENP1

• Plasmid: pSc-B-(GGSG X2) SENP1 (aa415-602)-(GGSG)-eGFP-SV40-SENP1 C603A (aa415-644)
• Parent Plasmid: pSC-B
• Species: Human

Sequence of Insert

AAGCTTGATAGTGAAGATGAATTTCCTGAAATTACAGAGGAAATGGAGAAAGAAATAAAGAATGTATTTCGTAATGGGAATCAGGATGAAGTTCTCAGTGAAGCATTTCGCCTGACCATTACACGCAAAGATATTCAAACTCTAAACCATCTGAATTGGCTCAATGATGAGATCATCAATTTCTACATGAATATGCTGATGGAGCGAAGTAAAGAGAAGGGCTTGCCAAGTGTGCATGCATTTAATACCTTTTTCTTCACTAAATTAAAAACGGCTGGTTATCAGGCAGTGAAACGTTGGACAAAGAAAGTAGATGTATTTTCTGTTGACATTCTTTTGGTGCCCATTCACCTGGGAGTACACTGGTGTCTAGCTGTTGTGGACTTTAGAAAGAAGAATATTACCTATTACGACTCCATGGGTGGGATAAACAATGAAGCCTGCAGAATACTCTTGCAATACCTAAAGCAAGAAAGCATTGACAAGAAAAGGAAAGAGTTTGACACCAATGGCTGGCAGCTTTTCAGCAAGAAAAGCCAGGAGATTCCTCAGCAGATGAATGGAAGTGACGCTGGGATGTTTGCCTGCAAATATGCTGACTGTATTACCAAAGACAGACCAATCAACTTCACACAGCAACACATGCCATACTTCCGGAAGCGGATGGTCTGGGAGATCCTCCACCGAAAACTCTTGTAGGTCGAC

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU24760
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: cloned overlap PCR product - SENP1 fragment with 5' and 3' linkers fused to eGFP-SV40 sequence. C603A SENP1 fragment then inserted into HindIII-Sal1 sites. 33bp of pSc-B sequence exists between nt1570-1601 (NW3414/3415 to remove). Entire SENP-eGFP-SV40-SENP C603A cassette can be excised with BamHI-Sal1.