cDNA Clone USP1

• Synonyms: UBP
• Plasmid: pFBDM6P1 USP1 S13-L785(end) delta 229-408 and 608-737 C90S/WDR48 M1-N563
• Parent Plasmid: pFBDM6P1
• Expressed: GST-3C-USP1 S13-L785(end) delta 229-408 and 608-737 C90S
• Genbank: NM_001017415.1 GI:63053521
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCTCAAGAGGTAGCCCTTCAAAGAAAAACAGACTTTCCTTAAAGTTTTTTCAGAAAAAGGAAACTAAGAGAGCTTTGGATTTCACAGATTCTCAAGAAAATGAAGAAAAAGCTTCTGAATATAGAGCATCTGAAATTGATCAAGTTGTTCCTGCAGCACAGTCTTCACCTATAAACTGTGAGAAGAGAGAAAACTTGTTACCATTTGTGGGACTGAATAATCTCGGCAATACTAGCTATCTTAATAGTATACTTCAGGTATTATATTTTTGTCCCGGTTTTAAATCTGGAGTAAAGCACTTATTTAATATTATTTCAAGGAAGAAAGAAGCTCTAAAGGATGAAGCCAATCAAAAAGACAAGGGAAATTGCAAAGAAGATTCTTTGGCAAGTTATGAATTGATATGCAGTTTACAGTCCTTAATCATTTCGGTTGAACAGCTCCAGGCTAGTTTTCTCTTAAATCCAGAGAAATATACTGATGAACTTGCCACTCAGCCAAGGCGACTGCTTAACACACTGAGGGAACTCAACCCTATGTATGAAGGATATCTACAGCATGATGCACAGGAAGTATTACAATGTATTTTGGGAAACATTCAAGAAACATGCCAACTCCTAAAAAAAGAAGAAGTAAAAAATGTGGCAGAAAATGAAGTTAAACCCATAAACAAAGGTGAAGAACAAATTGGTTTTGAGCTAGTGGAGAAATTATTTCAAGGTCAGCTGGTATTAAGGACGCGTTGCTTGGAATGTGAAAGTTTAACAGAAAGAAGAGAAGATTTTCAAGACATCAGTGTGCCAGTACAAGAAGATGAGCTTTCCAAAGTAGAGGAGAGTTCTGAAATTTCTCCAGAGCCAAAAACAGAAATGAAGACCCTGAGATGGGCAATTTCACAATTTGCTTCAGTAGAAAGGATTGTAGGAGAAGATAAATATTTCTGTGAAAACTGCCATCATTATACTGAAGCTGAACGAAGTCTTTTGTTTGACAAAATGCCTGAAGTTATAACTATTCATTTGAAGTGCTTTGCTGCTAGTGGTTTGGAGTTTGATTGTTATGGTGGTGGACTTTCCAAGATCAACACTCCTTTATTGACACCTCTTAAATTGTCACTAGAAGAATGGAGCACAAAGCCAACTAACGACAGCTATGGATTATTTGCGGTTGTGATGCATAGTGGCATTACAATTAGTAGTGGGCATTACACTGCTTCTGTTAAAGTCACTGACCTTAACAGTTTAGAACAAAGCTTAAAGGAGTATGAGGGGAAGTGGTTGCTTTTTGATGATTCTGAAGTCAAAGTTACTGAAGAGAAGGACTTTCTGAATTCTCTTTCCCCTTCTACATCTCCTACTTCTACTCCTTACTTGCTATTTTATAAGAAATTATAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKASEYRASEIDQVVPAAQSSPINCEKRENLLPFV GLNNLGNTSYLNSILQVLYFCPGFKSGVKHLFNIISRKKEALKDEANQKDKGNCKEDSLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRL LNTLRELNPMYEGYLQHDAQEVLQCILGNIQETCQLLKKEEVKNVAENEVKPINKGEEQIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQ EDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLK LSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLEQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU53513
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: WDR48 M1-N563 NcoI/KpnI into DU53496