cDNA Clone Ufm1

• Plasmid: pMEX TEV HA Ufm1 [G83C]
• Parent Plasmid: pMEX
• Expressed: MBP-TEV-HA-Ufm1 [G83C]
• Genbank: P61960
• Tag: HA
• Tag: MBP

Sequence of Insert

GGATCCGAAAACCTGTATTTTCAGGGCATGTACCCATACGATGTGCCAGATTACGCCATGTCGAAGGTTTCCTTTAAGATCACGCTGACGTCGGACCCACGGCTGCCGTACAAAGTACTCAGTGTTCCTGAAAGTACACCTTTCACAGCAGTCTTAAAGTTTGCAGCAGAAGAATTTAAAGTTCCTGCTGCAACAAGTGCAATTATTACCAATGATGGAATAGGAATAAATCCTGCACAGACTGCTGGAAATGTTTTTCTAAAACATGGTTCAGAACTGCGGATTATTCCTAGAGATCGTGTTTGCAGTTGTTAAGCGAAGGGCGAATTC

Amino Acid Sequence

MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGPLGSENLYFQGMYPYDVPDYAMSKVSFKITLTSDPRLPYKVLSVPESTPFTAVLKFAAEEFKVPAATSAIITNDGIGINPAQTAGNVFLKHGSELRIIPRDRVCSC*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU55462
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: The His-tagged protein (DU55407) is not soluble. Please try to sub clone into a MPB-vector and do away with the His-tag. Thanks
• Low Temp: No