cDNA Clone RhoA

• Plasmid: pET15b 6HIS TEV HALO 3C RHOA
• Parent Plasmid: pET15b 6His TEV Halo 3C
• Expressed: 6HIS TEV HALO 3C RHOA
• Genbank: NM_001664.2 GI:50593005
• Tag: 6His
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCCCGGCTGCCATCCGGAAGAAACTGGTGATTGTTGGTGATGGAGC
CTGTGGAAAGACATGCTTGCTCATAGTTAACAGCAAGGACCAGTTCCCAG
AGGTGTATGTGCCCACAGTGTTTGAGAACTATGTGGCAGATATCGAGGTG
GATGGAAAGCAGGTAGAGTTGGCTTTGTGGGACACAGCTGGGCAGGAAGA
TTATGATCGCCTGAGGCCCCTCTCCTACCCAGATACCGATGTTATACTGA
TGTGTTTTTCCATCGACAGCCCTGATAGTTTAGAAAACATCCCAGAAAAG
TGGACCCCAGAAGTCAAGCATTTCTGTCCCAACGTGCCCATCATCCTGGT
TGGGAATAAGAAGGATCTTCGGAATGATGAGCACACAAGGCGGGAGCTAG
CCAAGATGAAGCAGGAGCCGGTGAAACCTGAAGAAGGCAGAGATATGGCA
AACAGGATTGGCGCTTTTGGGTACATGGAGTGTTCAGCAAAGACCAAAGA
TGGAGTGAGAGAGGTTTTTGAAATGGCTACGAGAGCTGCTCTGCAAGCTA
GACGTGGGAAGAAAAAATCTGGGTGCCTTGTCTTGTGAAAGCTGGGAATT
C

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSENLYFQGMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMG
KSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIE
GTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPG
LNLLQEDNPDLIGSEIARWLSTLEISGLEVLFQGPGSPAAIRKKLVIVGDGACGKTCLLIVNSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQ
EDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
KDGVREVFEMATRAALQARRGKKKSGCLVL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU56125
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 47992 Daltons
• Low Temp: No