cDNA Clone USP35

• Plasmid: pcDNA FRT TO Rhod ext-USP35 51-1018
• Parent Plasmid: pcDNA FRT TO
• Expressed: SRMNGTEGPNFYVPFSNKTVD-USP35 51-1018

Sequence of Insert

GGATCCAGCAGAATGAACGGGACCGAGGGCCCAAACTTCTACGTGCCTTTCTCCAACAAGACGGTGGACATGGCCAGACACCCCAGATGCGTGCCCGACGGCCCCCACAGACTGCTGTTCTGTCAGCAGCTCGTGCGGTGCCTGGGCCGGTTCAGATGTCCTGCCGAAGGCGAAGAGGGCGCCGTGGAATTCCTGGAACAGGCCCAGCAGGTCAGCGGCCTGCTGGCACAGCTGTGGCGAGCCCAGCCTGCCGCCATCCTGCCCTGCCTGAAAGAACTGTTCGCCGTGATCAGCTGCGCCGAGGAAGAACCCCCTAGCAGCGCTCTGGCCAGCGTGGTGCAGCATCTGCCTCTGGAACTGATGGACGGCGTCGTGCGGAACCTGAGCAACGACGACAGCGTGACCGACAGCCAGATGCTGACCGCCATCAGCCGGATGATCGACTGGGTGTCCTGGCCCCTGGGCAAGAACATCGACAAGTGGATCATTGCCCTGCTGAAGGGCCTGGCCGCCGTGAAGAAGTTCAGCATCCTGATCGAGGTGTCCCTGACCAAGATCGAGAAGGTGTTCAGCAAGCTGCTGTACCCCATCGTGCGGGGAGCCGCCCTGAGCGTGCTGAAGTACATGCTGCTGACCTTCCAGCACAGCCACGAGGCCTTCCATCTGCTGCTGCCCCACATCCCCCCCATGGTGGCCAGCCTGGTCAAAGAGGACAGCAACAGCGGCACCAGCTGCCTGGAACAGCTGGCCGAGCTGGTGCACTGCATGGTGTTCCGGTTCCCCGGCTTCCCCGACCTGTACGAGCCCGTGATGGAAGCCATCAAGGACCTGCACGTGCCCAACGAGGACCGGATCAAGCAGCTGCTGGGCCAGGACGCCTGGACCAGCCAGAAGTCCGAGCTGGCCGGCTTCTACCCCAGGCTGATGGCCAAGAGCGACACCGGCAAGATCGGCCTGATCAACCTGGGCAATACCTGCTACGTGAACAGCATCCTGCAGGCCCTGTTCATGGCCAGCGACTTCCGGCACTGCGTGCTGCGGCTGACCGAGAACAACAGCCAGCCCCTGATGACCAAGCTGCAGTGGCTGTTCGGCTTTCTGGAACACTCCCAGAGGCCCGCTATCAGCCCCGAGAACTTCCTGAGCGCCAGCTGGACCCCCTGGTTCAGCCCTGGCACCCAGCAGGACTGCAGCGAGTACCTGAAGTATCTGCTGGACCGGCTGCACGAGGAAGAGAAAACCGGCACCCGGATCTGCCAGAAGCTGAAGCAGAGCAGCAGCCCCAGCCCCCCTGAGGAACCTCCTGCCCCAAGCAGCACCAGCGTGGAAAAGATGTTCGGCGGAAAGATCGTGACCAGAATCTGCTGCCTGTGCTGTCTGAACGTGTCCAGCCGGGAAGAGGCCTTCACCGACCTGAGCCTGGCCTTCCCACCCCCCGAGAGATGCAGAAGGCGGAGACTGGGCAGCGTGATGCGGCCCACCGAGGACATCACCGCCAGAGAGCTGCCTCCCCCAACAAGCGCCCAGGGACCTGGCAGAGTGGGCCCCAGACGGCAGCGGAAGCACTGCATCACCGAGGATACCCCCCCCACCAGCCTGTACATCGAGGGCCTGGACAGCAAAGAGGCTGGCGGCCAGAGCAGCCAGGAAGAGAGAATCGAGCGCGAGGAAGAGGGCAAAGAGGAACGCACCGAGAAAGAGGAAGTCGGCGAGGAAGAAGAGAGCACCAGAGGCGAGGGCGAGCGCGAAAAAGAGGAAGAAGTCGAAGAGGAAGAGGAAAAGGTCGAGAAAGAGACAGAGAAAGAGGCCGAGCAGGAAAAAGAAGAGGACTCCCTGGGAGCCGGCACCCACCCCGATGCCGCCATTCCTAGCGGCGAGAGAACCTGCGGCAGCGAGGGCAGCAGAAGCGTGCTGGACCTGGTCAACTACTTCCTGTCCCCCGAGAAGCTGACCGCCGAGAACCGGTACTACTGCGAGAGCTGCGCCAGCCTGCAGGACGCCGAGAAGGTGGTGGAACTGAGCCAGGGCCCCTGCTACCTGATCCTGACCCTGCTGAGATTCAGCTTCGACCTGCGGACCATGCGGCGGAGAAAGATTCTGGACGACGTGTCCATCCCCCTGCTGCTGAGGCTGCCTCTGGCTGGCGGAAGAGGCCAGGCCTACGACCTGTGCAGCGTGGTGGTGCACAGCGGCGTGTCCAGCGAGAGCGGCCACTACTACTGCTACGCCAGAGAGGGCGCTGCCAGACCAGCCGCCTCTCTGGGCACAGCCGATAGACCCGAGCCCGAGAACCAGTGGTATCTGTTCAACGACACCAGAGTGTCCTTCAGCAGCTTCGAGAGCGTGTCCAACGTGACCTCATTCTTCCCAAAGGACACCGCCTACGTGCTGTTCTACCGGCAGCGGCCTAGAGAGGGCCCTGAGGCCGAACTGGGCAGCTCCAGAGTGCGGACCGAGCCCACCCTGCACAAGGACCTGATGGAAGCTATCTCCAAGGACAACATCCTGTATCTGCAGGAACAGGAAAAAGAGGCCAGAAGCAGAGCCGCCTACATCAGCGCCCTGCCCACCAGCCCTCACTGGGGCAGAGGCTTCGACGAGGACAAGGATGAGGACGAGGGCAGCCCTGGCGGCTGCAACCCTGCTGGCGGCAATGGCGGCGACTTCCACCGGCTGGTGTTCTGATGAGCGGCCGC

Amino Acid Sequence

SRMNGTEGPNFYVPFSNKTVDMARHPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVEFLEQAQQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVVQHLPLELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWIIALLKGLAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHSHEAFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGFPDLYEPVMEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSDTGKIGLINLGNTCYVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWLFGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHPDAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCASLQDAEKVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPLAGGRGQAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPENQWYLFNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGSSRVRTEPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGRGFDEDKDEDEGSPGGCNPAGGNGGDFHRLVF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU55843
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please clone USP35 51-1018 with an N-terminal SRMNGTEGPNFYVPFSNKTVD tag into pcDNA5 FRT/TO hygro. Thank you.
• Low Temp: No