cDNA Clone USP35

• Plasmid: pcDNA FRT TO USP35-GFP 180-1018
• Parent Plasmid: pcDNA FRT TO C-GFP
• Expressed: USP35-GFP 180-1018
• Tag: GFP

Sequence of Insert

GGATCCATGGAAGGCGAAGAGGGCGCCGTGGAATTCCTGGAACAGGCCCAGCAGGTCAGCGGCCTGCTGGCACAGCTGTGGCGAGCCCAGCCTGCCGCCATCCTGCCCTGCCTGAAAGAACTGTTCGCCGTGATCAGCTGCGCCGAGGAAGAACCCCCTAGCAGCGCTCTGGCCAGCGTGGTGCAGCATCTGCCTCTGGAACTGATGGACGGCGTCGTGCGGAACCTGAGCAACGACGACAGCGTGACCGACAGCCAGATGCTGACCGCCATCAGCCGGATGATCGACTGGGTGTCCTGGCCCCTGGGCAAGAACATCGACAAGTGGATCATTGCCCTGCTGAAGGGCCTGGCCGCCGTGAAGAAGTTCAGCATCCTGATCGAGGTGTCCCTGACCAAGATCGAGAAGGTGTTCAGCAAGCTGCTGTACCCCATCGTGCGGGGAGCCGCCCTGAGCGTGCTGAAGTACATGCTGCTGACCTTCCAGCACAGCCACGAGGCCTTCCATCTGCTGCTGCCCCACATCCCCCCCATGGTGGCCAGCCTGGTCAAAGAGGACAGCAACAGCGGCACCAGCTGCCTGGAACAGCTGGCCGAGCTGGTGCACTGCATGGTGTTCCGGTTCCCCGGCTTCCCCGACCTGTACGAGCCCGTGATGGAAGCCATCAAGGACCTGCACGTGCCCAACGAGGACCGGATCAAGCAGCTGCTGGGCCAGGACGCCTGGACCAGCCAGAAGTCCGAGCTGGCCGGCTTCTACCCCAGGCTGATGGCCAAGAGCGACACCGGCAAGATCGGCCTGATCAACCTGGGCAATACCTGCTACGTGAACAGCATCCTGCAGGCCCTGTTCATGGCCAGCGACTTCCGGCACTGCGTGCTGCGGCTGACCGAGAACAACAGCCAGCCCCTGATGACCAAGCTGCAGTGGCTGTTCGGCTTTCTGGAACACTCCCAGAGGCCCGCTATCAGCCCCGAGAACTTCCTGAGCGCCAGCTGGACCCCCTGGTTCAGCCCTGGCACCCAGCAGGACTGCAGCGAGTACCTGAAGTATCTGCTGGACCGGCTGCACGAGGAAGAGAAAACCGGCACCCGGATCTGCCAGAAGCTGAAGCAGAGCAGCAGCCCCAGCCCCCCTGAGGAACCTCCTGCCCCAAGCAGCACCAGCGTGGAAAAGATGTTCGGCGGAAAGATCGTGACCAGAATCTGCTGCCTGTGCTGTCTGAACGTGTCCAGCCGGGAAGAGGCCTTCACCGACCTGAGCCTGGCCTTCCCACCCCCCGAGAGATGCAGAAGGCGGAGACTGGGCAGCGTGATGCGGCCCACCGAGGACATCACCGCCAGAGAGCTGCCTCCCCCAACAAGCGCCCAGGGACCTGGCAGAGTGGGCCCCAGACGGCAGCGGAAGCACTGCATCACCGAGGATACCCCCCCCACCAGCCTGTACATCGAGGGCCTGGACAGCAAAGAGGCTGGCGGCCAGAGCAGCCAGGAAGAGAGAATCGAGCGCGAGGAAGAGGGCAAAGAGGAACGCACCGAGAAAGAGGAAGTCGGCGAGGAAGAAGAGAGCACCAGAGGCGAGGGCGAGCGCGAAAAAGAGGAAGAAGTCGAAGAGGAAGAGGAAAAGGTCGAGAAAGAGACAGAGAAAGAGGCCGAGCAGGAAAAAGAAGAGGACTCCCTGGGAGCCGGCACCCACCCCGATGCCGCCATTCCTAGCGGCGAGAGAACCTGCGGCAGCGAGGGCAGCAGAAGCGTGCTGGACCTGGTCAACTACTTCCTGTCCCCCGAGAAGCTGACCGCCGAGAACCGGTACTACTGCGAGAGCTGCGCCAGCCTGCAGGACGCCGAGAAGGTGGTGGAACTGAGCCAGGGCCCCTGCTACCTGATCCTGACCCTGCTGAGATTCAGCTTCGACCTGCGGACCATGCGGCGGAGAAAGATTCTGGACGACGTGTCCATCCCCCTGCTGCTGAGGCTGCCTCTGGCTGGCGGAAGAGGCCAGGCCTACGACCTGTGCAGCGTGGTGGTGCACAGCGGCGTGTCCAGCGAGAGCGGCCACTACTACTGCTACGCCAGAGAGGGCGCTGCCAGACCAGCCGCCTCTCTGGGCACAGCCGATAGACCCGAGCCCGAGAACCAGTGGTATCTGTTCAACGACACCAGAGTGTCCTTCAGCAGCTTCGAGAGCGTGTCCAACGTGACCTCATTCTTCCCAAAGGACACCGCCTACGTGCTGTTCTACCGGCAGCGGCCTAGAGAGGGCCCTGAGGCCGAACTGGGCAGCTCCAGAGTGCGGACCGAGCCCACCCTGCACAAGGACCTGATGGAAGCTATCTCCAAGGACAACATCCTGTATCTGCAGGAACAGGAAAAAGAGGCCAGAAGCAGAGCCGCCTACATCAGCGCCCTGCCCACCAGCCCTCACTGGGGCAGAGGCTTCGACGAGGACAAGGATGAGGACGAGGGCAGCCCTGGCGGCTGCAACCCTGCTGGCGGCAATGGCGGCGACTTCCACCGGCTGGTGTTCGCGGCCGCT

Amino Acid Sequence

MEGEEGAVEFLEQAQQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVVQHLPLELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWIIALLKGLAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHSHEAFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGFPDLYEPVMEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSDTGKIGLINLGNTCYVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWLFGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHPDAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCASLQDAEKVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPLAGGRGQAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPENQWYLFNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGSSRVRTEPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGRGFDEDKDEDEGSPGGCNPAGGNGGDFHRLVFAAAMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU55776
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please clone USP35 180-1018 with a C-terminal GFP tag into pcDNA5 FRT/TO hygro. Thank you.
• Low Temp: No