cDNA Clone RN213

• Plasmid: pCMV-HA-RN213 aa3980-5207
• Parent Plasmid: pCMV HA
• Expressed: HA-RN213 aa3980-5207
• Genbank: Q63HN8
• Tag: HA
• Species: Human

Sequence of Insert

agatctGAATGCAAGGAGACAGCCAGCAAGACCCTCAGCAGGTTTGGGAT
TCAGCCGTGCTCCATCTGCCTGGGAGATGCAAAGGACCCCGTCTGTCTGC
CCTGCGACCACGTGCACTGCCTGCGCTGCCTCAGGGCCTGGTTTGCCTCA
GAGCAGATGATATGCCCCTACTGTTTAACTGCCTTGCCAGACGAATTCTC
TCCAGCTGTTTCCCAAGCGCACAGGGAAGCCATTGAAAAGCATGCCCGCT
TCCGGCAGATGTGCAACAGTTTCTTCGTAGACCTGGTGTCCACCATTTGC
TTCAAGGACAACGCTCCGCCTGAGAAGGAAGTGATTGAGAGCCTGCTCTC
TCTCCTCTTCGTCCAAAAGGGGCGCTTAAGAGATGCTGCCCAGAGACACT
GTGAACACACAAAATCTCTCTCTCCATTCAATGATGTTGTGGATAAGACT
CCTGTCATCCGCTCAGTGATACTGAAACTGCTTTTGAAGTACAGCTTTCA
TGATGTAAAAGATTATATTCAGGAATATTTGACCCTGTTAAAAAAGAAAG
CATTCATAACTGAAGATAAAACTGAACTGTACATGCTCTTCATCAACTGC
CTGGAGGATTCAATACTTGAGAAGACCAGTGCTTACTCCAGAAATGATGA
ACTGAACCACCTAGAAGAGGAAGGTCGTTTCCTTAAGGCATATTCTCCAG
CAAGCCGGGGCCGAGAGCCTGCCAACGAGGCCTCGGTTGAATACCTGCAA
GAGGTGGCCCGGATCCGCCTCTGCCTCGACAGAGCTGCAGATTTCCTCTC
GGAGCCTGAGGGAGGCCCAGAGATGGCCAAGGAGAAGCAGTGCTACCTGC
AGCAAGTCAAGCAGTTCTGTATCCGGGTGGAGAACGACTGGCACCGGGTG
TACCTGGTGCGGAAGCTCAGCAGCCAGCGGGGGATGGAGTTCGTGCAGGG
CCTCTCCAAGCCCGGCCGCCCGCACCAGTGGGTGTTTCCCAAGGACGTTG
TCAAGCAGCAGGGGCTGCGGCAGGACCACCCAGGCCAGATGGATAGGTAC
CTGGTGTACGGCGATGAATACAAGGCTCTCCGTGATGCTGTGGCCAAAGC
TGTCCTCGAGTGCAAGCCACTGGGCATTAAGACTGCTCTGAAGGCCTGCA
AGACCCCCCAAAGCCAGCAGTCAGCCTACTTCCTGTTAACACTGTTTAGA
GAGGTGGCTATTTTGTACAGATCCCACAATGCAAGCCTCCACCCCACGCC
AGAGCAATGTGAAGCTGTGAGCAAATTCATTGGCGAATGCAAGATCCTTT
CACCTCCTGATATCAGCCGTTTTGCAACATCGCTCGTGGACAATTCTGTG
CCATTGTTGAGGGCGGGGCCTAGTGACAGCAACCTTGATGGAACGGTGAC
AGAAATGGCCATTCATGCTGCAGCCGTCCTTCTGTGTGGACAGAATGAAC
TCTTGGAGCCCCTAAAGAATCTGGCCTTCTCCCCAGCCACCATGGCGCAT
GCTTTTCTTCCAACCATGCCTGAAGACTTGCTGGCTCAAGCTCGGAGGTG
GAAGGGTCTGGAGCGAGTCCACTGGTACACTTGTCCCAACGGCCATCCTT
GCTCCGTGGGAGAGTGTGGCAGGCCGATGGAACAGAGCATCTGCATTGAC
TGCCATGCGCCGATTGGAGGCATTGACCACAAACCTCGGGACGGCTTTCA
TCTGGTCAAAGACAAGGCAGACAGAACGCAGACCGGCCATGTGCTGGGCA
ACCCGCAGCGGAGAGACGTGGTGACATGTGACCGAGGGCTGCCCCCAGTG
GTCTTCCTCCTTATCCGGCTACTCACTCACTTGGCTCTGCTTCTGGGAGC
GTCCCAGAGTTCCCAGGCTCTGATAAACATCATTAAGCCTCCAGTGAGGG
ATCCAAAAGGCTTTCTGCAGCAGCACATCCTGAAGGACCTGGAGCAGTTG
GCCAAGATGCTGGGACACAGTGCCGACGAGACCATCGGCGTGGTCCACCT
CGTCCTGCGCAGGCTTCTCCAAGAGCAGCACCAGCTCTCTAGCAGAAGGC
TTTTAAATTTTGACACAGAATTGTCAACTAAAGAAATGAGGAACAACTGG
GAAAAGGAAATCGCAGCTGTGATTTCTCCTGAACTGGAGCATCTAGATAA
AACCCTTCCCACCATGAATAATCTCATCAGCCAAGATAAGCGTATCAGCT
CTAACCCTGTGGCCAAAATAATATATGGTGACCCAGTGACCTTCCTGCCC
CACCTGCCCCGGAAAAGTGTGGTCCATTGCTCTAAGATTTGGAGCTGCAG
GAAAAGAATTACAGTTGAGTACCTCCAGCACATTGTGGAACAGAAAAATG
GCAAAGAAAGAGTGCCCATCCTCTGGCATTTCCTGCAGAAGGAAGCAGAG
CTGAGGCTGGTAAAGTTCCTGCCTGAGATTTTGGCCTTGCAAAGGGATCT
AGTGAAGCAGTTCCAGAACGTCCAGCAAGTTGAATACAGCTCCATCAGAG
GCTTCCTCAGCAAGCACAGCTCAGATGGGTTGAGGCAGCTGCTTCACAAC
AGGATCACAGTCTTTCTGTCCACATGGAACAAACTGAGGAGATCGCTTGA
GACGAACGGTGAGATCAACCTACCCAAAGACTACTGCAGCACTGACTTGG
ATCTGGACACTGAGTTTGAGATCCTCTTGCCACGCCGACGGGGCCTGGGC
CTCTGTGCTACCGCTCTCGTCAGCTACTTGATTCGCCTACACAATGAAAT
TGTCTACGCCGTGGAAAAACTCTCCAAGGAAAACAACAGCTATTCCGTGG
ATGCCGCCGAGGTCACTGAACTGCATGTCATCAGTTATGAAGTGGAGCGG
GACCTGACTCCACTGATTCTCTCCAACTGCCAGTACCAGGTGGAGGAGGG
CAGAGAGACCGTGCAGGAGTTCGATCTGGAGAAGATTCAGCGGCAGATCG
TCAGCCGCTTCCTCCAGGGCAAGCCCCGGCTGAGCCTCAAGGGAATACCC
ACTCTGGTGTACAGACACGACTGGAACTATGAACATCTCTTTATGGACAT
CAAGAACAAAATGGCACAGGACTCCCTCCCCAGCTCGGTCATTAGTGCCA
TCAGTGGACAGCTGCAGTCCTACAGCGATGCCTGTGAAGTGCTGTCTGTC
GTAGAAGTCACTCTGGGGTTTCTGAGCACAGCTGGTGGGGATCCAAACAT
GCAGCTGAATGTGTATACTCAAGACATCCTGCAAATGGGTGATCAGACGA
TTCACGTGTTAAAGGCCTTAAACAGATGCCAGTTAAAACACACCATTGCC
CTCTGGCAGTTCCTGTCTGCTCATAAGTCTGAACAGCTGCTGCGGCTGCA
CAAAGAGCCATTTGGGGAAATCAGTTCAAGGTACAAAGCGGATCTGAGCC
CGGAAAATGCTAAGCTCCTCAGCACATTCCTAAATCAGACTGGCCTAGAC
GCCTTCCTGCTAGAGCTGCACGAAATGATAATCTTGAAACTAAAGAACCC
CCAAACCCAAACCGAGGAGCGCTTCCGCCCTCAGTGGAGCCTGAGAGACA
CTCTCGTAAGTTACATGCAAACTAAAGAAAGTGAAATTCTTCCTGAAATG
GCATCTCAGTTCCCAGAAGAGATACTGCTCGCCAGCTGTGTCTCAGTGTG
GAAAACAGCTGCTGTGCTGAAATGGAATCGAGAAATGAGATAGgcggccg
c

Amino Acid Sequence

MYPYDVPDYAGSECKETASKTLSRFGIQPCSICLGDAKDPVCLPCDHVHC
LRCLRAWFASEQMICPYCLTALPDEFSPAVSQAHREAIEKHARFRQMCNS
FFVDLVSTICFKDNAPPEKEVIESLLSLLFVQKGRLRDAAQRHCEHTKSL
SPFNDVVDKTPVIRSVILKLLLKYSFHDVKDYIQEYLTLLKKKAFITEDK
TELYMLFINCLEDSILEKTSAYSRNDELNHLEEEGRFLKAYSPASRGREP
ANEASVEYLQEVARIRLCLDRAADFLSEPEGGPEMAKEKQCYLQQVKQFC
IRVENDWHRVYLVRKLSSQRGMEFVQGLSKPGRPHQWVFPKDVVKQQGLR
QDHPGQMDRYLVYGDEYKALRDAVAKAVLECKPLGIKTALKACKTPQSQQ
SAYFLLTLFREVAILYRSHNASLHPTPEQCEAVSKFIGECKILSPPDISR
FATSLVDNSVPLLRAGPSDSNLDGTVTEMAIHAAAVLLCGQNELLEPLKN
LAFSPATMAHAFLPTMPEDLLAQARRWKGLERVHWYTCPNGHPCSVGECG
RPMEQSICIDCHAPIGGIDHKPRDGFHLVKDKADRTQTGHVLGNPQRRDV
VTCDRGLPPVVFLLIRLLTHLALLLGASQSSQALINIIKPPVRDPKGFLQ
QHILKDLEQLAKMLGHSADETIGVVHLVLRRLLQEQHQLSSRRLLNFDTE
LSTKEMRNNWEKEIAAVISPELEHLDKTLPTMNNLISQDKRISSNPVAKI
IYGDPVTFLPHLPRKSVVHCSKIWSCRKRITVEYLQHIVEQKNGKERVPI
LWHFLQKEAELRLVKFLPEILALQRDLVKQFQNVQQVEYSSIRGFLSKHS
SDGLRQLLHNRITVFLSTWNKLRRSLETNGEINLPKDYCSTDLDLDTEFE
ILLPRRRGLGLCATALVSYLIRLHNEIVYAVEKLSKENNSYSVDAAEVTE
LHVISYEVERDLTPLILSNCQYQVEEGRETVQEFDLEKIQRQIVSRFLQG
KPRLSLKGIPTLVYRHDWNYEHLFMDIKNKMAQDSLPSSVISAISGQLQS
YSDACEVLSVVEVTLGFLSTAGGDPNMQLNVYTQDILQMGDQTIHVLKAL
NRCQLKHTIALWQFLSAHKSEQLLRLHKEPFGEISSRYKADLSPENAKLL
STFLNQTGLDAFLLELHEMIILKLKNPQTQTEERFRPQWSLRDTLVSYMQ
TKESEILPEMASQFPEEILLASCVSVWKTAAVLKWNREMR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29341
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Silent changes compared to NCBI ref H578 CAC > CAT; Parental 44059
• Low Temp: No