cDNA Clone UFM1

• Plasmid: pSc-B-UFM1 (aa1-80) T3C intein overlap
• Parent Plasmid: pSC-B
• Species: Human

Sequence of Insert

GCTAGCATGGGTTGCTCTTCCGGCTCGAAGGTTTCCTTTAAGATCACGCTGACGTCGGACCCACGGCTGCCGTACAAAGTACTCAGTGTTCCTGAAAGTACACCTTTCACAGCAGTCTTAAAGTTTGCAGCAGAAGAATTTAAAGTTCCTGCTGCAACAAGTGCAATTATTACCAATGATGGAATAGGAATAAATCCTGCACAGACTGCTGGAAATGTTTTTCTAAAACATGGTTCAGAACTGCGGATTATTCCTAGAGATTGCATCTGCGGAGATGCACTAGT

Amino Acid Sequence

MGCSSGSKVSFKITLTSDPRLPYKVLSVPESTPFTAVLKFAAEEFKVPAA
TSAIITNDGIGINPAQTAGNVFLKHGSELRIIPRDCICGDAL

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU29459
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GCSSG inserted after Met1 and 3' end overlaps intein (to Spe1 site) and includes T3C mutation. No stop. For cloning into Nhe1 Spe1 sites of pTXB1
• Low Temp: No