cDNA Clone UBD (FAT10)

• Plasmid: pSc-B-FAT10 aa1-162 (C7S C9S C134S C160S C162S)-T3C intein overlap
• Parent Plasmid: pSC-B
• Genbank: O15205
• Species: Human

Sequence of Insert

GCTAGCATGGGTTGCTCTTCCGGCGCTCCCAATGCTTCCTCTCTCTCTGTGCATGTCCGTTCCGAGGAATGGGATTTAATGACCTTTGATGCCAACCCATATGACAGCGTGAAAAAAATCAAAGAACATGTCCGGTCTAAGACCAAGGTTCCTGTGCAGGACCAGGTTCTTTTGCTGGGCTCCAAGATCTTAAAGCCACGGAGAAGCCTCTCATCTTATGGCATTGACAAAGAGAAGACCATCCACCTTACCCTGAAAGTGGTGAAGCCCAGTGATGAGGAGCTGCCCTTGTTTCTTGTGGAGTCAGGTGATGAGGCAAAGAGGCACCTCCTCCAGGTGCGAAGGTCCAGCTCAGTGGCACAAGTGAAAGCAATGATCGAGACTAAGACGGGTATAATCCCTGAGACCCAGATTGTGACTTCCAATGGAAAGAGACTGGAAGATGGGAAGATGATGGCAGATTATGGCATCAGAAAGGGCAACTTACTCTTCCTGGCATCTTATTCTTGCATCTGCGGAGATGCACTAGT

Amino Acid Sequence

MGCSSGAPNASSLSVHVRSEEWDLMTFDANPYDSVKKIKEHVRSKTKVPV
QDQVLLLGSKILKPRRSLSSYGIDKEKTIHLTLKVVKPSDEELPLFLVES
GDEAKRHLLQVRRSSSVAQVKAMIETKTGIIPETQIVTSNGKRLEDGKMM
ADYGIRKGNLLFLASYSCICGDAL

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU29471
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GCSSG inserted after Met1 and 3' end overlaps intein (to Spe1 site) and includes T3C mutation. No stop. For cloning into Nhe1 Spe1 sites of pTXB1
• Low Temp: No