cDNA Clone USP25

• Plasmid: pcDNA FRT TO GFP USP25 C178A
• Expressed: GFP-USP25 C178A
• Genbank: NM_013396.3
• Tag: GFP

Sequence of Insert

ggatccATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAA GCACCAGCAGACGTTTTTGAATCAACTGAGAGAAATTACGGGGATTAATG ACACCCAGATACTACAGCAAGCCTTGAAGGATAGTAATGGAAACTTGGAA TTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAGACCCCTCAGCAGGA GGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACATCA GTGTGGGAAGCCAAGCAGATACAAATGTGATTGATCTCACTGGAGATGAT AAAGATGATCTTCAGAGAGCAATTGCCTTGAGTTTGGCCGAATCAAACAG GGCATTCAGGGAGACTGGAATAACTGATGAGGAACAAGCCATTAGCAGAG TTCTTGAAGCCAGCATAGCAGAGAATAAAGCATGTTTGAAGAGGACACCT ACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGATAGAAAAAGACAGGA CAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTgctTGGTTTAGTG CTGTTATTCAGTCATTATTTAATCTTTTGGAATTTAGAAGATTAGTTCTG AATTACAAGCCTCCATCAAATGCTCAAGATTTACCCCGAAACCAAAAGGA ACATCGGAATTTGCCTTTTATGCGTGAGCTGAGGTATCTATTTGCACTTC TTGTTGGTACCAAAAGGAAGTATGTTGATCCATCAAGAGCAGTTGAAATT CTTAAGGATGCTTTCAAATCAAATGACTCACAGCAGCAAGATGTGAGTGA GTTTACACACAAATTATTAGATTGGTTAGAAGATGCCTTCCAAATGAAAG CTGAAGAGGAGACGGATGAAGAGAAGCCAAAGAACCCCATGGTAGAGTTG TTCTATGGCAGATTCCTGGCTGTGGGAGTACTTGAAGGTAAAAAATTTGA AAACACTGAAATGTTTGGTCAGTACCCACTTCAGGTCAATGGGTTCAAAG ATCTGCATGAGTGCCTAGAAGCTGCAATGATTGAAGGAGAAATTGAGTCT TTACATTCAGAGAATTCAGGAAAATCAGGCCAAGAGCATTGGTTTACTGA ATTACCACCTGTGTTAACATTTGAATTGTCAAGATTTGAATTTAATCAGG CATTGGGAAGACCAGAAAAAATTCACAACAAATTAGAATTTCCCCAAGTT TTATATTTGGACAGATACATGCACAGAAACAGAGAAATAACAAGAATTAA GAGGGAAGAGATCAAGAGACTGAAAGATTACCTCACGGTATTACAACAAA GGCTAGAAAGATATTTAAGCTATGGTTCCGGTCCCAAACGATTCCCCTTG GTAGATGTTCTTCAGTATGCATTGGAATTTGCCTCAAGTAAACCTGTTTG CACTTCTCCTGTTGACGATATTGACGCTAGTTCCCCACCTAGTGGTTCCA TACCATCACAGACATTACCAAGCACAACAGAACAACAGGGAGCCCTATCT TCAGAACTGCCAAGCACATCACCTTCATCAGTTGCTGCCATTTCATCGAG ATCAGTAATACACAAACCATTTACTCAGTCCCGGATACCTCCAGATTTGC CCATGCATCCGGCACCGAGGCACATAACGGAGGAAGAACTTTCTGTGCTG GAAAGTTGTTTACATCGCTGGAGGACAGAAATAGAAAATGACACCAGAGA TTTGCAGGAAAGCATATCCAGAATCCATCGAACAATTGAATTAATGTACT CTGACAAATCTATGATACAAGTTCCTTATCGATTACATGCCGTTTTAGTT CACGAAGGCCAAGCTAATGCTGGGCACTACTGGGCATATATTTTTGATCA TCGTGAAAGCAGATGGATGAAGTACAATGATATTGCTGTGACAAAATCAT CATGGGAAGAGCTAGTGAGGGACTCTTTTGGTGGTTATAGAAATGCCAGT GCATACTGTTTAATGTACATAAATGATAAGGCACAGTTCCTAATACAAGA GGAGTTTAATAAAGAAACTGGGCAGCCCCTTGTTGGTATAGAAACATTAC CACCGGATTTGAGAGATTTTGTTGAGGAAGACAACCAACGATTTGAAAAA GAACTAGAAGAATGGGATGCACAACTTGCCCAGAAAGCTTTGCAGGAAAA GCTTTTAGCGTCTCAGAAATTGAGAGAGTCAGAGACTTCTGTGACAACAG CACAAGCAGCAGGAGACCCAGAATATCTAGAGCAGCCATCAAGAAGTGAT TTCTCAAAGCACTTGAAAGAAGAAACTATTCAAATAATTACCAAGGCATC ACATGAGCATGAAGATAAAAGTCCTGAAACAGTTTTGCAGTCGGCAATTA AGTTGGAATATGCAAGGTTGGTTAAGTTGGCCCAAGAAGACACCCCACCA GAAACCGATTATCGTTTACATCATGTAGTGGTCTACTTTATCCAGAACCA GGCACCAAAGAAAATTATTGAGAAAACATTACTAGAACAATTTGGAGATA GAAATTTGAGTTTTGATGAAAGGTGTCACAACATAATGAAAGTTGCTCAA GCCAAACTGGAAATGATAAAACCTGAAGAAGTAAACTTGGAGGAATATGA GGAGTGGCATCAGGATTATAGGAAATTCAGGGAAACAACTATGTATCTCA TAATTGGGCTAGAAAATTTTCAAAGAGAAAGTTATATAGATTCCTTGCTG TTCCTCATCTGTGCTTATCAGAATAACAAAGAACTCTTGTCTAAAGGCTT ATACAGAGGACATGATGAAGAATTGATATCACATTATAGAAGAGAATGTT TGCTAAAATTAAATGAGCAAGCCGCAGAACTCTTCGAATCTGGAGAGGAT CGAGAAGTAAACAATGGTTTGATTATCATGAATGAGTTTATTGTCCCATT TTTGCCATTATTACTGGTGGATGAAATGGAAGAAAAGGATATACTAGCTG TAGAAGATATGAGAAATCGATGGTGTTCCTACCTTGGTCAAGAAATGGAA CCACACCTCCAAGAAAAGCTGACAGATTTTTTGCCAAAACTGCTTGATTG TTCTATGGAGATTAAAAGTTTCCATGAGCCACCGAAGTTACCTTCATATT CCACGCATGAACTCTGTGAGCGATTTGCCCGAATCATGTTGTCCCTCAGT CGAACTCCTGCTGATGGAAGATAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMTVEQNVLQQSAAQKHQQTFLNQLREITGINDTQILQQALKDSNGNLELAVAFLTAKNAKTPQQEETTYYQTALPGNDRYISVGSQADTNVIDLTGDDKDDLQRAIALSLAESNRAFRETGITDEEQAISRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTAWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMHRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPVDDIDASSPPSGSIPSQTLPSTTEQQGALSSELPSTSPSSVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHRESRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQPLVGIETLPPDLRDFVEEDNQRFEKELEEWDAQLAQKALQEKLLASQKLRESETSVTTAQAAGDPEYLEQPSRSDFSKHLKEETIQIITKASHEHEDKSPETVLQSAIKLEYARLVKLAQEDTPPETDYRLHHVVVYFIQNQAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU59618
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please clone DU Number:49617 with a point mutation at C178 to Alanine
• Low Temp: No