cDNA Clone VRP1

• Plasmid: pRS413 ADH Pr 400-VRP1/YLR337C-200 S519/522E
• Parent Plasmid: pRS413 ADH Pr
• Expressed: VRP1/YLR337C S519/522E
• Genbank: 851051

Sequence of Insert

GGATCCTCCGCTACATTGCTGTATTCTCTTTTAAGTTCAAATTACAAGGGCAAGATAAAAATTTTAGCTCACAAAAGATGGAAAAAGTCGCAAATATTCAAGTTACGAAACATTGACAGAGGATGGAATCACAATGTTAGCCTTCAAATCCTACTATCCTGTATGAAGCTTGCTAAAAGCGTCTTTAGAAAGAATGAAATGTTCATAACCTAGTCACTGCTTACGGGTGCGGCCGCCGTCCTCAAATAGGGGCCTTAAGAATAATCCGCACAGTCAGTAAATACTTGAACAAAATCTAAAATTATAAACTTATGAAGAAAGTTTTTGATATGAGACAAAGCATACGACTAAAAAGCGCACAACCGTTTGTTGAAGAAGCCTACCATATATATAACGAATTGTTGAAATGGCAGGTGCTCCAGCTCCTCCTCCTCCACCGCCTCCACCAGCATTAGGTGGTAGTGCTCCTAAACCGGCAAAATCTGTGATGCAGGGCCGTGACGCCCTGTTAGGTGACATTAGAAAGGGAATGAAACTAAAGAAAGCTGAGACCAATGATAGAAGTGCCCCAATCGTGGGAGGTGGTGTCGTTTCATCGGCCTCAGGAAGTTCTGGTACCGTCAGTAGTAAAGGACCCTCTATGTCAGCTCCTCCAATCCCTGGGATGGGCGCTCCGCAGTTGGGCGATATTTTAGCTGGAGGTATACCGAAGTTAAAACATATCAATAACAATGCAAGCACTAAACCCTCTCCATCGGCGAGCGCACCGCCAATTCCGGGCGCGGTACCATCTGTTGCGGCTCCCCCCATTCCTAATGCACCATTATCACCAGCACCCGCTGTTCCTAGTATTCCCTCGTCTTCTGCACCTCCAATTCCTGATATTCCATCTTCTGCGGCCCCTCCTATTCCCATTGTACCATCATCTCCAGCACCACCGCTTCCATTATCAGGTGCCTCTGCTCCAAAGGTACCGCAAAATAGACCGCATATGCCTTCAGTAAGGCCAGCACACAGAAGCCACCAAAGAAAAAGCTCTAATATTTCTCTTCCTAGTGTGTCAGCACCCCCTTTGCCTTCAGCGTCTCTGCCAACACATGTTAGCAACCCTCCACAAGCTCCACCCCCTCCCCCAACTCCAACAATTGGCCTGGACTCAAAAAACATTAAACCTACAGATAACGCAGTCTCACCACCATCTTCTGAAGTTCCTGCAGGCGGGTTGCCATTTTTGGCAGAAATTAATGCCAGGAGGTCTGAACGAGGAGCCGTCGAGGGCGTTTCTTCCACAAAGATTCAAACCGAAAATCATAAATCTCCTTCACAACCACCACTTCCATCATCTGCTCCGCCCATTCCTACATCACATGCTCCGCCATTACCGCCTACTGCACCACCTCCACCATCGTTACCAAATGTGACCTCTGCACCTAAAAAAGCAACTTCAGCTCCAGCTCCGCCTCCACCACCACTACCAGCAGCCATGATCTCTGCGTCGACTAACAGCGTAAAAGCAACCCCGGTTCCCCCAACTTTAGCACCACCGTTACCAAATACTACATCAGTTCCTCCTAACAAAGCATCGTCAATGCCCGCGCCACCACCTCCTCCTCCCCCACCACCTGGTGCTTTTTCAACATCCTCTGCCTTGTCAGCATCATCTATTCCTTTAGCACCACTACCACCTCCTCCTCCTCCTTCGGTTGCAACGTCAGTACCATCGGCTCCTCCTCCTCCTCCAACACTCACGACAAACAAACCCTCAGCGTCCAGTAAGCAATCCAAAATATCTTCATCCAGTTCTTCCTCGGCCGTAACACCTGGGGGACCCTTACCATTTCTTGCTGAGATCCAAAAGAAGAGAGATGATAGATTCGTGGTTGGTGGTGATACTGGATACACTACCCAAGATAAACAAGAGGATGTAATAGGTTCATCTAAGGATGATAACGTAAGACCTGAACCTATTGAGCCATCTATAAACCCGCCTAAACAGAGTAGCCAAAATGGTATGTCGTTTCTGGATGAAATTGAATCCAAATTACATAAGCAAACATCATCGAATGCTTTTAACGCACCACCCCCCCATACGGATGCTATGGCGCCTCCTTTGCCTCCTAGTGCACCTCCTCCTCCTATAACATCGCTACCGACTCCAACTGCATCAGGTGATGACCACACCAATGATAAGAGTGAGACTGTGTTAGGGATGAAAAAAGCAAAGGCCCCTGCACTGCCTGGACACGTTCCACCTCCACCAGTTCCACCAGTTTTATCAGATGATAGCAAGAATAATGTCCCCGCCGCTTCTTTATTACACGATGTTCTTCCCTCCTCGAATCTCGAAAAACCTCCTTCTCCTCCTGTTGCAGCTGCTCCGCCATTGCCAACATTTAGTGCACCAAGTCTGCCTCAGCAATCCGTGTCTACATCAATACCATCACCTCCTCCTGTTGCGCCTACACTTTCCGTTCGTACCGAAACTGAATCAATTTCGAAGAACCCCACTAAGTCGCCACCCCCACCACCAAGTCCATCTACGATGGATACCGGTACCTCTAATAGCCCCAGTAAAAACCTTAAACAACGGTTATTTTCTACAGGTGGATCGACGCTGCAACACAAGCATAATACGCATACAAATCAACCAGATGTTGATGTAGGTAGGTATACGATAGGGGGATCGAATTCTATCGTTGGGGCCAAATCTGGAAATGAAAGAATTGTTATAGATGATTCAAGATTTAAATGGACTAATGTGTCACAAATGCCTAAGCCACGTCCCTTTCAAAATAAAACAAAACTGTATCCAAGTGGAAAGGGTAGTAGTGTGCCATTGGACTTAACATTATTTACGTGAGCATTTCTCCATGGTTACAATAAATCACTGAAGAACAAGAAAATAATAATATTTTATAAAAATATTTTACTAAATTACATATGGTATCTGCAATATTTATTTTTTAATCTTTTAGCTTCCCGTCCTTTTCGCTGTGAAAAATATAAAATCCAGCAAAAAAAAAAAAATGCGATGAGCTTACAAGACTACTGAACTTACTTCTCGAG

Amino Acid Sequence

MAGAPAPPPPPPPPALGGSAPKPAKSVMQGRDALLGDIRKGMKLKKAETNDRSAPIVGGGVVSSASGSSGTVSSKGPSMSAPPIPGMGAPQLGDILAGGIPKLKHINNNASTKPSPSASAPPIPGAVPSVAAPPIPNAPLSPAPAVPSIPSSSAPPIPDIPSSAAPPIPIVPSSPAPPLPLSGASAPKVPQNRPHMPSVRPAHRSHQRKSSNISLPSVSAPPLPSASLPTHVSNPPQAPPPPPTPTIGLDSKNIKPTDNAVSPPSSEVPAGGLPFLAEINARRSERGAVEGVSSTKIQTENHKSPSQPPLPSSAPPIPTSHAPPLPPTAPPPPSLPNVTSAPKKATSAPAPPPPPLPAAMISASTNSVKATPVPPTLAPPLPNTTSVPPNKASSMPAPPPPPPPPPGAFSTSSALSASSIPLAPLPPPPPPSVATSVPSAPPPPPTLTTNKPSASSKQSKISSSSSSSAVTPGGPLPFLAEIQKKRDDRFVVGGDTGYTTQDKQEDVIGSSKDDNVRPEPIEPSINPPKQSSQNGMSFLDEIESKLHKQTSSNAFNAPPPHTDAMAPPLPPSAPPPPITSLPTPTASGDDHTNDKSETVLGMKKAKAPALPGHVPPPPVPPVLSDDSKNNVPAASLLHDVLPSSNLEKPPSPPVAAAPPLPTFSAPSLPQQSVSTSIPSPPPVAPTLSVRTETESISKNPTKSPPPPPSPSTMDTGTSNSPSKNLKQRLFSTGGSTLQHKHNTHTNQPDVDVGRYTIGGSNSIVGAKSGNERIVIDDSRFKWTNVSQMPKPRPFQNKTKLYPSGKGSSVPLDLTLFT*

• Vector Type: Yeast
• Antibiotic: Amp
• DU Number: DU66150
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: VRP1 - S519A/S522E from pRS413 ADH Pr 400-VRP1/YLR337C-200 (DU59830) changes to reference sequence: silent g781a and del P444
• Low Temp: No