cDNA Clone TEV Protease

• Plasmid: pcDNA5 FRT/TO-10His-TEV Nla Protease S219V
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: 10His-TEV Nla Protease S219V
• Genbank: NP_734212
• Tag: 6His
• Species: Tobacco Etch Virus

Sequence of Insert

GGATCCATGGGCCATCATCATCATCATCATCATCATCATCACGGAGGTATGGGCGAGAGCTTGTTTAAGGGACCACGTGATTACAACCCGATATCGAGCACCATTTGTCATTTGACGAATGAATCTGATGGGCACACAACATCGTTGTATGGTATTGGATTTGGTCCCTTCATCATTACAAACAAGCACTTGTTTAGAAGAAATAATGGAACACTGTTGGTCCAATCACTACATGGTGTATTCAAGGTCAAGAACACCACGACTTTGCAACAACACCTCATTGATGGGAGGGACATGATAATTATTCGCATGCCTAAGGATTTCCCACCATTTCCTCAAAAGCTGAAATTTAGAGAGCCACAAAGGGAAGAGCGCATATGTCTTGTGACAACCAACTTCCAAACTAAGAGCATGTCTAGCATGGTGTCAGACACTAGTTGCACATTCCCTTCATCTGATGGCATATTCTGGAAGCATTGGATTCAAACCAAGGATGGGCAGTGTGGCAGTCCATTAGTATCAACTAGAGATGGGTTCATTGTTGGTATACACTCAGCATCGAATTTCACCAACACAAACAATTATTTCACAAGCGTGCCGAAAAACTTCATGGAATTGTTGACAAATCAGGAGGCGCAGCAGTGGGTTAGTGGTTGGCGATTAAATGCTGACTCAGTATTGTGGGGGGGCCATAAAGTTTTCATGGTCAAACCTGAAGAGCCTTTTCAGCCAGTTAAGGAAGCGACTCAACTCATGAATGAATTGGTGTACTCGCAATAAGCGGCCGC

Amino Acid Sequence

MGHHHHHHHHHHGGMGESLFKGPRDYNPISSTICHLTNESDGHTTSLYGI
GFGPFIITNKHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIII
RMPKDFPPFPQKLKFREPQREERICLVTTNFQTKSMSSMVSDTSCTFPSS
DGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFTNTNNYFTSVPKN
FMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPEEPFQPVKEATQLM
NELVYSQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65275
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: S219V to prevent self-proteolysis; The Nla-Pro protein (NP_734212.1) represents Gly2038-Q2279 of the polyprotein NP_062908.1; Parental 41459
• Low Temp: No