cDNA Clone SUMO1

• Synonyms: DAP1, SENP2, GMP1, PIC1, SMT3, UBL1, OFC10, SMT3C, SMT3H3
• Plasmid: pTXB1-SUMO1 C52S delta G97-Mxe Intein-CBD
• Parent Plasmid: pTXB1
• Expressed: SUMO1 C52S delta G97-Mxe Intein-CBD
• Genbank: Gene ID: 7341 NM_003352.4 → NP_003343.1
• Species: Human

Sequence of Insert

CATATGAGCGATCAAGAAGCAAAACCGAGCACCGAAGATCTGGGTGATAA
AAAAGAAGGCGAGTATATCAAACTGAAAGTGATTGGTCAGGATAGCAGCG
AAATCCATTTTAAAGTGAAAATGACCACCCATCTGAAAAAACTGAAAGAA
AGCTATTcTCAGCGTCAGGGTGTTCCGATGAATAGCCTGCGTTTTCTGTT
TGAAGGTCAGCGTATTGCAGATAATCATACCCCGAAAGAACTGGGTATGG
AAGAGGAAGATGTGATCGAAGTTTATCAAGAACAGACCGGTTGCATCACG
GGAGATGCACTAGTTGCCCTACCCGAGGGCGAGTCGGTACGCATCGCCGA
CATCGTGCCGGGTGCGCGGCCCAACAGTGACAACGCCATCGACCTGAAAG
TCCTTGACCGGCATGGCAATCCCGTGCTCGCCGACCGGCTGTTCCACTCC
GGCGAGCATCCGGTGTACACGGTGCGTACGGTCGAAGGTCTGCGTGTGAC
GGGCACCGCGAACCACCCGTTGTTGTGTTTGGTCGACGTCGCCGGGGTGC
CGACCCTGCTGTGGAAGCTGATCGACGAAATCAAGCCGGGCGATTACGCG
GTGATTCAACGCAGCGCATTCAGCGTCGACTGTGCAGGTTTTGCCCGCGG
GAAACCCGAATTTGCGCCCACAACCTACACAGTCGGCGTCCCTGGACTGG
TGCGTTTCTTGGAAGCACACCACCGAGACCCGGACGCCCAAGCTATCGCC
GACGAGCTGACCGACGGGCGGTTCTACTACGCGAAAGTCGCCAGTGTCAC
CGACGCCGGCGTGCAGCCGGTGTATAGCCTTCGTGTCGACACGGCAGACC
ACGCGTTTATCACGAACGGGTTCGTCAGCCACGCTACTGGCCTCACCGGT
CTGAACTCAGGCCTCACGACAAATCCTGGTGTATCCGCTTGGCAGGTCAA
CACAGCTTATACTGCGGGACAATTGGTCACATATAACGGCAAGACGTATA
AATGTTTGCAGCCCCACACCTCCTTGGCAGGATGGGAACCATCCAACGTT
CCTGCCTTGTGGCAGCTTCAATGACTGCAG

Amino Acid Sequence

MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKE
SYSQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGCIT
GDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPVLADRLFHS
GEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYA
VIQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIA
DELTDGRFYYAKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHATGLTG
LNSGLTTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
PALWQLQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pTXB1-SUMO1 C52S delta G97-Mxe Intein-CBD51.84 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65423
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: small ubiquitin-related modifier 1 isoform a precursor; codon optimised for bacterial expression
• Low Temp: No