cDNA Clone SUMO1

• Synonyms: DAP1, SENP2, GMP1, PIC1, SMT3, UBL1, OFC10, SMT3C, SMT3H3
• Plasmid: pTXB1-SUMO1 (Met1 GCSSG-Ser2) C52S delta G97-Mxe Intein-CBD
• Parent Plasmid: pTXB1
• Expressed: SUMO1 (Met1 GCSSG-Ser2) C52S delta G97-Mxe Intein-CBD
• Genbank: Gene ID: 7341 NM_003352.4 → NP_003343.1
• Species: Human

Sequence of Insert

CATATGGGTTGCTCTTCCGGCAGCGATCAAGAAGCAAAACCGAGCACCGA
AGATCTGGGTGATAAAAAAGAAGGCGAGTATATCAAACTGAAAGTGATTG
GTCAGGATAGCAGCGAAATCCATTTTAAAGTGAAAATGACCACCCATCTG
AAAAAACTGAAAGAAAGCTATTCTCAGCGTCAGGGTGTTCCGATGAATAG
CCTGCGTTTTCTGTTTGAAGGTCAGCGTATTGCAGATAATCATACCCCGA
AAGAACTGGGTATGGAAGAGGAAGATGTGATCGAAGTTTATCAAGAACAG
ACCGGTTGCATCACGGGAGATGCACTAGTTGCCCTACCCGAGGGCGAGTC
GGTACGCATCGCCGACATCGTGCCGGGTGCGCGGCCCAACAGTGACAACG
CCATCGACCTGAAAGTCCTTGACCGGCATGGCAATCCCGTGCTCGCCGAC
CGGCTGTTCCACTCCGGCGAGCATCCGGTGTACACGGTGCGTACGGTCGA
AGGTCTGCGTGTGACGGGCACCGCGAACCACCCGTTGTTGTGTTTGGTCG
ACGTCGCCGGGGTGCCGACCCTGCTGTGGAAGCTGATCGACGAAATCAAG
CCGGGCGATTACGCGGTGATTCAACGCAGCGCATTCAGCGTCGACTGTGC
AGGTTTTGCCCGCGGGAAACCCGAATTTGCGCCCACAACCTACACAGTCG
GCGTCCCTGGACTGGTGCGTTTCTTGGAAGCACACCACCGAGACCCGGAC
GCCCAAGCTATCGCCGACGAGCTGACCGACGGGCGGTTCTACTACGCGAA
AGTCGCCAGTGTCACCGACGCCGGCGTGCAGCCGGTGTATAGCCTTCGTG
TCGACACGGCAGACCACGCGTTTATCACGAACGGGTTCGTCAGCCACGCT
ACTGGCCTCACCGGTCTGAACTCAGGCCTCACGACAAATCCTGGTGTATC
CGCTTGGCAGGTCAACACAGCTTATACTGCGGGACAATTGGTCACATATA
ACGGCAAGACGTATAAATGTTTGCAGCCCCACACCTCCTTGGCAGGATGG
GAACCATCCAACGTTCCTGCCTTGTGGCAGCTTCAATGACTGCAG

Amino Acid Sequence

MGCSSGSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLK
KLKESYSQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQT
GCITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPVLADR
LFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKP
GDYAVIQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDA
QAIADELTDGRFYYAKVASVTDAGVQPVYSLRVDTADHAFITNGFVSHAT
GLTGLNSGLTTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWE
PSNVPALWQLQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pTXB1-SUMO1 (Met1 GCSSG-Ser2) C52S delta G97-Mxe Intein-CBD55.59 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65454
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: GCSSG inserted between Met1 and Ser2 of SUMO1 ORF; small ubiquitin-related modifier 1 isoform a precursor; codon optimised for bacterial expression
• Low Temp: No