cDNA Clone RPM-1 6

• Synonyms: -
• Plasmid: pGEX6P1 RPM-1 D3420-F3766
• Parent Plasmid: pGEX6P1
• Expressed: GST-RPM-1 D3420-F3766 (end)
• Genbank: NP_505267.1, NM_072866.4
• Tag: GST
• Species: Caenorhabditis elegans

Sequence of Insert

GGATCCATGGACACTCTCTTTCCCACGTCAACTTCTTCCATACAACCGGCAATGCAAAGTTCAAGTGCATTTCTTGGCATCCATCCCATGACATGTCGGTTCTGTGGAAATAATGTTCCAGTGGAAGATCAGAGTCTTGATGGAACTTGTACTCATGAGGATTGTGTTAATTATGCAAAAACCGCGTGTCAAGTAATGCATACTTGCAACCATTTCTGTGGTGGAATTCGAAACGAAGAAGAATGTCTTCCGTGTATGACCTGTAAACGAGAAGATGCAGCTCAAGATGGAGACGATGTTTGTGTAATTTGTTTCACTGAAAGACTTGGAGCAGCACCATGTATTCGCCTCGGATGTGGTCATATGTTCCATTTCCATTGTGTTCGAATGATTTTGGAAAGAAGATGGAATGGTCCAAGAATTGTTTTCCGTTTTATGCAGTGTCCATTGTGCATTCAACCTATTGAACATTCTGGATTACAAGATCTTATCGAACCATTGAAAACAATTCGACAAGAAGTTGTAGATAAAGCAAAAATGCGTTTGGAATATGATGGTCTCCTTACAACTCCAGCTCTAACTGATCCAAGATCCGAGTTTTACAATCAACCAGAGGAATATGCTCTTGATAGATATATGTATGTCTTGTGTCACAAGTGTAAGAAAGCCTACTTTGGTGGAGAAAGTCGTTGTCAAGCCGCATTAGATAGCTCGCAGTTCAATCCTGAAGAATTACTATGTGGTGGTTGTTCAGATACAAGCGGAGTTCAAGTCTGCCCAAGACATGGAGTCGAATATCTGGAGTACAAATGCCGATTCTGTTGCTCTATTGCTGTTTATTTCTGTTTTGGTACAACTCATTTCTGCGCACCTTGCCATGATGACTTCCAGCGTTTAATGTCTCTTCCAAAACATCTTCTTCCAACATGTCCAGTTGGCCCTCGATCAACACCAATGGAAGAACAAACATGCCCCCTGAAGATGAAGCATCCTCCAACTGGTGATGAATTTGCAATGGGTTGTGGAATTTGTAGAAATATTAGTACTTTTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMDTLFPTSTSSIQPAMQSSSAFLGIHPMTCRFCGNNVPVEDQSLDGTCTHEDCVNYAKTACQVMHTCNHFCGGIRNEEECLPCMTCKREDAAQDGDDVCVICFTERLGAAPCIRLGCGHMFHFHCVRMILERRWNGPRIVFRFMQCPLCIQPIEHSGLQDLIEPLKTIRQEVVDKAKMRLEYDGLLTTPALTDPRSEFYNQPEEYALDRYMYVLCHKCKKAYFGGESRCQAALDSSQFNPEELLCGGCSDTSGVQVCPRHGVEYLEYKCRFCCSIAVYFCFGTTHFCAPCHDDFQRLMSLPKHLLPTCPVGPRSTPMEEQTCPLKMKHPPTGDEFAMGCGICRNISTF*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 RPM-1 D3420-F3766136.07 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU66358
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: We would like a longer construct as RPM-1 aa3555-3766 does not include the RING domain of this E3. Please can we order a synthetic construct to add residues 3420-3555, so that the final construct is GST-3C-aa3420-3766.
• Low Temp: No