cDNA Clone Ufc1

• Plasmid: pGEX6P1 Ufc1(mouse)(K0) C116K
• Parent Plasmid: pGEX6P1
• Expressed: GST-C3-UFC1 (K0) C116K
• Genbank: Q9CR09 NM_025388.2
• Tag: GST

Sequence of Insert

GGATCCATGGCGGACGAGGCCACTCGGCGGGTCGTGTCTGAGATCCCGGTGCTGAGGACCAACGCCGGACCCCGAGATCGGGAATTGTGGGTGCAGCGACTAAGGGAGGAATATCAGTCCCTGATCCGGTATGTGGAGAACAACAGGAATTCGGACAATGATTGGTTCCGACTGGAGTCCAACAGGGAAGGGACCCGGTGGTTTGGAAGATGCTGGTACATCCACGATTTCCTCAGATATGAGTTCGACATCGAGTTTGAAATTCCTATCACATATCCCACTACTGCTCCGGAAATTGCAGTCCCTGAGCTGGACGGGAGGACAGCAAGGATGTACAGGGGTGGCAGAATAAAGCTGACTGATCATTTCAGACCTTTGTGGGCCAGGAATGTGCCTAGGTTTGGACTAGCTCATCTCATGGCCCTGGGGCTGGGTCCTTGGCTGGCAGTGGAAGTCCCCGATCTGATCCAGAGGGGTGTGATCCAGCACAGAGAAAGATGCAGCCAATGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMADEATRRVVSEIPVLRTNAGPRDRELWVQRLREEYQSLIRYVENNRNSDNDWFRLESNREGTRWFGRCWYIHDFLRYEFDIEFEIPITYPTTAPEIAVPELDGRTARMYRGGRIKLTDHFRPLWARNVPRFGLAHLMALGLGPWLAVEVPDLIQRGVIQHRERCSQ*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 Ufc1(mouse)(K0) C116K115.44 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU66383
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Kindly mutate Cysteine at 116 to Lysine on UFC1 K0 background. Please use 59746 construct as a template! Many thanks in advance! K0=K17R, K33R , K47R ,K60R ,K68R ,K77R ,K105R ,K108R ,K114R ,K122R ,K131R ,K156R ,K162R ,K164R
• Low Temp: No