cDNA Clone RAB5B

• Plasmid: pCMV5D HA RAB5B Mito (MAO tag)
• Parent Plasmid: pCMV5D HA mcs Mito
• Expressed: HA RAB5B Mito (MAO tag)
• Genbank: NM_002868.3
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGACTAGCAGAAGCACAGCTAGGCCCAATGGGCAACCCCAGGCCAGCAAAATTTGCCAGTTCAAATTGGTCCTGCTGGGAGAATCTGCAGTGGGAAAGTCAAGCCTGGTATTACGTTTTGTCAAAGGGCAGTTCCATGAGTACCAGGAGAGCACCATTGGAGCGGCCTTCCTCACCCAGTCCGTTTGTCTAGATGACACAACAGTGAAGTTTGAGATCTGGGACACAGCTGGGCAGGAGCGATATCACAGCTTAGCCCCCATGTACTACAGGGGTGCCCAAGCTGCAATCGTGGTTTACGACATTACTAATCAGGAAACCTTTGCCCGAGCAAAGACATGGGTGAAGGAACTACAGCGACAGGCCAGTCCTAGCATCGTTATTGCCCTGGCAGGGAACAAAGCTGACCTGGCCAACAAACGTATGGTGGAGTATGAAGAGGCCCAGGCATATGCAGATGACAACAGCTTATTGTTCATGGAGACTTCAGCCAAGACAGCTATGAACGTGAATGATCTCTTCCTGGCAATAGCTAAGAAGTTGCCAAAGAGTGAACCCCAGAATCTGGGAGGTGCAGCAGGCCGAAGCCGGGGTGTGGATCTCCATGAACAGTCCCAGCAGAACAAGAGCCAGGGTGCTGGCGCCGGTGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMTSRSTARPNGQPQASKICQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQSVCLDDTTVKFEIWDTAGQERYHSLAPM
YYRGAQAAIVVYDITNQETFARAKTWVKELQRQASPSIVIALAGNKADLANKRMVEYEEAQAYADDNSLLFMETSAKTAMNVNDLFLAIAKKLPKSEPQN
LGGAAGRSRGVDLHEQSQQNKSQGAGAGAAAFWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5D HA RAB5B Mito (MAO tag)918.24 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61288
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: HA Rab5B 1-211 (CCSN residues removed at the C-terminus) fused to 3 alanines and a C terminal MAO fragment 489-end (FWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS).
• Low Temp: No