cDNA Clone RAB8A

• Synonyms: MEL; RAB8
• Plasmid: pCMV5D HA RAB8A Mito (MAO tag)
• Parent Plasmid: pCMV5D HA mcs Mito
• Expressed: HA RAB8A Mito (MAO tag)
• Genbank: NM_005370.4
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGCGAAGACCTACGATTACCTGTTCAAGCTGCTGCTGATCGGGGACTCGGGGGTGGGGAAGACCTGTGTCCTGTTCCGCTTCTCCGAGGACGCCTTCAACTCCACTTTTATCTCCACCATAGGAATTGACTTTAAAATTAGGACCATAGAGCTCGATGGCAAGAGAATTAAACTGCAGATATGGGACACAGCCGGTCAGGAACGGTTTCGGACGATCACAACGGCCTACTACAGGGGTGCAATGGGCATCATGCTGGTCTACGACATCACCAACGAGAAGTCCTTCGACAACATCCGGAACTGGATTCGCAACATTGAGGAGCACGCCTCTGCAGACGTCGAAAAGATGATACTCGGGAACAAGTGTGATGTGAATGACAAGAGACAAGTTTCCAAGGAACGGGGAGAAAAGCTGGCCCTCGACTATGGAATCAAGTTCATGGAGACCAGCGCGAAGGCCAACATCAATGTGGAAAATGCATTTTTCACTCTCGCCAGAGATATCAAAGCAAAAATGGACAAAAAATTGGAAGGCAACAGCCCCCAGGGGAGCAACCAGGGAGTCAAAATCACACCGGACCAGCAGAAGAGGAGCAGCTTTTTCCGAGGTGCTGGCGCCGGTGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVY
DITNEKSFDNIRNWIRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSNQG
VKITPDQQKRSSFFRGAGAGAAAFWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5D HA RAB8A Mito (MAO tag)822.85 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61286
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: HA Rab8A 1-203 (CVLL residues removed at the C-terminus) fused to 3 alanines and a C terminal MAO fragment 489-end (FWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS).
• Low Temp: No