cDNA Clone PPM1H

• Synonyms: ARHCL1; NERPP-2C; URCC2
• Plasmid: pET15b 6His SUMO PPM1H M1-E309
• Parent Plasmid: pET15b 6His SUMO
• Expressed: 6His SUMO PPM1H M1-E309
• Genbank: NM_020700.2
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

GGATCCATGCTCACTCGAGTGAAATCTGCCGTGGCCAATTTCATGGGCGGCATCATGGCTGGCAGCTCAGGCTCCGAGCACGGCGGCGGCAGCTGCGGAGGCTCGGACCTGCCCCTGCGTTTCCCCTACGGGCGGCCAGAGTTCCTGGGGCTGTCTCAGGACGAGGTGGAGTGCAGCGCCGACCACATCGCCCGCCCCATCCTCATCCTCAAGGAGACTCGGCGGCTGCCCTGGGCCACTGGCTACGCAGAGGTTATCAATGCCGGGAAGAGCACACACAATGAAGACCAAGCCAGCTGTGAGGTGCTCACTGTGAAGAAGAAGGCAGGGGCCGTGACCTCAACCCCAAACAGGAACTCATCCAAGAGACGGTCCTCCCTTCCCAATGGGGAAGGGCTGCAGCTGAAGGAGAACTCGGAATCCGAGGGTGTTTCCTGCCACTATTGGTCGCTGTTTGACGGGCACGCGGGGTCCGGGGCCGCGGTGGTGGCGTCACGCCTGCTGCAGCACCACATCACGGAGCAGCTGCAGGACATCGTGGACATCCTGAAGAACTCCGCCGTCCTGCCCCCTACCTGCCTGGGGGAGGAGCCTGAGAACACGCCCGCCAACAGCCGGACTCTGACCCGGGCAGCCTCCCTGCGCGGAGGGGTGGGGGCCCCGGGCTCCCCCAGCACGCCCCCCACACGCTTCTTTACCGAGAAGAAGATTCCCCATGAGTGCCTGGTCATCGGAGCGCTTGAAAGTGCATTCAAGGAAATGGACCTACAGATAGAACGAGAGAGGAGTTCATATAATATATCTGGTGGCTGCACGGCCCTCATTGTGATTTGCCTTTTGGGGAAGCTGTATGTTGCAAATGCTGGGGATAGCAGGGCCATAATCATCAGAAATGGAGAAATTATCCCCATGTCTTCAGAATTTACCCCCGAGTGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSMLTRVKSAVANFMGGIMAGSSGSEHGGGSCGGSDLPLRFPYGRPEFLGLSQDEVECSADHIARPILILKETRRLPWATGYAEVINAGKSTHNEDQ
ASCEVLTVKKKAGAVTSTPNRNSSKRRSSLPNGEGLQLKENSESEGVSCHYWSLFDGHAGSGAAVVASRLLQHHITEQLQDIVDILKNSAVLPPTCLGEE
PENTPANSRTLTRAASLRGGVGAPGSPSTPPTRFFTEKKIPHECLVIGALESAFKEMDLQIERERSSYNISGGCTALIVICLLGKLYVANAGDSRAIIIR
NGEIIPMSSEFTPE*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET15b 6His SUMO PPM1H M1-E30988.45 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61328
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: SUMO is not blunt in this clone.
• Low Temp: No