cDNA Clone PPM1H

• Plasmid: pET15b 6HIS SUMO PPM1H S33-end K88A D288A
• Parent Plasmid: p~ET15b 6HIS SUMO
• Expressed: 6HIS SUMO PPM1H S33-end K88A D288A
• Genbank: Q9ULR3.2
• Tag: 6His
• Tag: SUMO
• Species: Human

Sequence of Insert

TCGGACCTGCCCCTGCGTTTCCCCTACGGGCGGCCAGAGTTCCTGGGGCTGTCTCAGGACGAGGTGGAGTGCAGCGCCGACCACATCGCCCGCCCCATCCTCATCCTCAAGGAGACTCGGCGGCTGCCCTGGGCCACTGGCTACGCAGAGGTTATCAATGCCGGGgcGAGCACACACAATGAAGACCAAGCCAGCTGTGAGGTGCTCACTGTGAAGAAGAAGGCAGGGGCCGTGACCTCAACCCCAAATAGGAACTCATCCAAGAGACGGTCCTCCCTTCCCAATGGGGAAGGGCTGCAGCTGAAGGAGAACTCGGAATCCGAGGGTGTTTCCTGCCACTATTGGTCGCTGTTTGACGGGCACGCGGGGTCCGGGGCCGCGGTGGTGGCGTCACGCCTGCTGCAGCACCACATCACGGAGCAGCTGCAGGACATCGTGGACATCCTGAAGAACTCCGCCGTCCTGCCCCCTACCTGCCTGGGGGAGGAGCCTGAGAACACGCCCGCCAACAGCCGGACTCTGACCCGGGCAGCCTCCCTGCGCGGAGGGGTGGGGGCCCCGGGCTCCCCCAGCACGCCCCCCACACGCTTCTTTACCGAGAAGAAGATTCCCCATGAGTGCCTGGTCATCGGAGCGCTTGAAAGTGCATTCAAGGAAATGGACCTACAGATAGAACGAGAGAGGAGTTCATATAATATATCTGGTGGCTGCACGGCCCTCATTGTGATTTGCCTTTTGGGGAAGCTGTATGTTGCAAATGCTGGGGcTAGCAGGGCCATAATCATCAGAAATGGAGAAATTATCCCCATGTCTTCAGAATTTACCCCCGAGACGGAGCGCCAGCGACTTCAGTACCTGGCATTCATGCAGCCTCACTTGCTGGGAAATGAGTTCACACATTTGGAGTTTCCAAGGAGAGTACAGAGAAAGGAGCTTGGAAAGAAGATGCTCTACAGGGACTTTAATATGACAGGCTGGGCATACAAAACCATTGAGGATGAGGACTTGAAGTTCCCCCTTATATATGGAGAAGGCAAGAAGGCCCGGGTAATGGCAACTATTGGAGTGACCAGGGGACTTGGGGACCATGACCTGAAGGTGCATGACTCCAACATCTACATTAAACCATTCCTGTCTTCAGCTCCAGAGGTAAGAATCTACGATCTTTCAAAATATGATCATGGATCAGATGATGTGCTGATCTTGGCCACTGATGGACTCTGGGACGTTTTATCAAATGAAGAAGTAGCAGAAGCAATCACTCAGTTTCTTCCTAACTGTGATCCAGATGATCCTCACAGGTACACACTGGCAGCTCAGGACCTGGTGATGCGTGCCCGGGGTGTGCTGAAGGACAGAGGATGGCGGATATCTAATGACCGACTGGGCTCAGGAGACGACATTTCTGTATATGTCATTCCTTTAATACATGGAAACAAGCTGTCATGAGCGGCCGC

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSDLPLRFPYGRPEFLGLSQDEVECSADHIARPILILKETRRLPWATGYAEVINAGASTHNEDQASCEVLTVKKKAGAVTSTPNRNSSKRRSSLPNG
EGLQLKENSESEGVSCHYWSLFDGHAGSGAAVVASRLLQHHITEQLQDIVDILKNSAVLPPTCLGEEPENTPANSRTLTRAASLRGGVGAPGSPSTPPTR
FFTEKKIPHECLVIGALESAFKEMDLQIERERSSYNISGGCTALIVICLLGKLYVANAGASRAIIIRNGEIIPMSSEFTPETERQRLQYLAFMQPHLLGN
EFTHLEFPRRVQRKELGKKMLYRDFNMTGWAYKTIEDEDLKFPLIYGEGKKARVMATIGVTRGLGDHDLKVHDSNIYIKPFLSSAPEVRIYDLSKYDHGS
DDVLILATDGLWDVLSNEEVAEAITQFLPNCDPDDPHRYTLAAQDLVMRARGVLKDRGWRISNDRLGSGDDISVYVIPLIHGNKLS*AA

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET15b 6HIS SUMO PPM1H S33-end K88A D288A376.19 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU68664
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No