cDNA Clone TRAF6

• Synonyms: TNF receptor-associated factor 6
• Plasmid: pBabeD puro HA-TRAF6 KallR
• Parent Plasmid: pBabeD puro HA
• Expressed: human TRAF6 KallR
• Genbank: NM_145803.3 → NP_665802.1
• Tag: HA
• Species: Human

Sequence of Insert

ggatccATGAGTCTGCTAAACTGTGAAAACAGCTGTGGATctAGCCAGTCTGAAAGTGACTGCTGTGTGGCCATGGCCAGCTCCTGTAGCGCTGTAACAAgAGATGATAGTGTGGGTGGAACTGCCAGCACGGGGAACCTCTCCAGCTCATTTATGGAGGAGATCCAGGGATATGATGTAGAGTTTGACCCACCCCTGGAAAGCAgGTATGAATGCCCCATCTGCTTGATGGCATTACGAGAAGCAGTGCAAACGCCATGCGGCCATAGGTTCTGCAgAGCCTGCATCATAAgATCAATAAGGGATGCAGGTCACAgATGTCCAGTTGACAATGAAATACTGCTGGAAAATCAACTATTTCCAGACAATTTTGCAAgACGTGAGATTCTTTCTCTGATGGTGAgATGTCCAAATGAAGGTTGTTTGCACAgGATGGAACTGAGACATCTTGAGGATCATCAAGCACATTGTGAGTTTGCTCTTATGGATTGTCCCCAATGCCAGCGTCCCTTCCAAAgATTCCATATTAATATTCACATTCTGAgGGATTGTCCAAGGAGACAGGTTTCTTGTGACAACTGTGCTGCATCAATGGCATTTGAAGATAgAGAGATCCATGACCAGAACTGTCCTTTGGCAAATGTCATCTGTGAATACTGCAATACTATACTCATCAGAGAACAGATGCCTAATCATTATGATCTAGACTGCCCTACAGCCCCAATTCCATGCACATTCAGTACTTTTGGTTGCCATGAAAgGATGCAGAGGAATCACTTGGCACGCCACCTACAAGAGAACACCCAGTCACACATGAGAATGTTGGCCCAGGCTGTTCATAGTTTGAGCGTTATACCCGACTCTGGGTATATCTCAGAGGTCCGGAATTTCCAGGAAACTATTCACCAGTTAGAGGGTCGCCTTGTAAGACAAGACCATCAAATCCGGGAGCTGACTGCTAgAATGGAAACTCAGAGTATGTATGTAAGTGAGCTCAgACGAACCATTCGAACCCTTGAGGACAgAGTTGCTGAAATCGAAGCACAGCAGTGCAATGGAATTTATATTTGGAgGATTGGCAACTTTGGAATGCATTTGAgATGTCAAGAAGAGGAGAgACCTGTTGTGATTCATAGCCCTGGATTCTACACTGGCAgACCCGGGTACAgACTGTGCATGCGCTTGCACCTTCAGTTACCGACTGCTCAGCGCTGTGCAAACTATATATCCCTTTTTGTCCACACAATGCAAGGAGAATATGACAGCCACCTCCCTTGGCCCTTCCAGGGTACAATACGCCTTACAATTCTTGATCAGTCTGAAGCACCTGTAAGGCAAAACCACGAAGAGATAATGGATGCCAgACCAGAGCTGCTTGCTTTCCAGCGACCCACAATCCCACGGAACCCAAgAGGTTTTGGCTATGTAACTTTTATGCATCTGGAAGCCCTAAGACAAAGAACTTTCATTAgGGATGACACATTATTAGTGCGCTGTGAGGTCTCCACCCGCTTTGACATGGGTAGCCTTCGGAGGGAGGGTTTTCAGCCACGAAGTACTGATGCAGGGGTATAGGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMSLLNCENSCGSSQSESDCCVAMASSCSAVTRDDSVGGTASTGNLSSSFMEEIQGYDVEFDPPLESRYECPICLMALREAVQTPCGHRFCRACIIRSIRDAGHRCPVDNEILLENQLFPDNFARREILSLMVRCPNEGCLHRMELRHLEDHQAHCEFALMDCPQCQRPFQRFHINIHILRDCPRRQVSCDNCAASMAFEDREIHDQNCPLANVICEYCNTILIREQMPNHYDLDCPTAPIPCTFSTFGCHERMQRNHLARHLQENTQSHMRMLAQAVHSLSVIPDSGYISEVRNFQETIHQLEGRLVRQDHQIRELTARMETQSMYVSELRRTIRTLEDRVAEIEAQQCNGIYIWRIGNFGMHLRCQEEERPVVIHSPGFYTGRPGYRLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDARPELLAFQRPTIPRNPRGFGYVTFMHLEALRQRTFIRDDTLLVRCEVSTRFDMGSLRREGFQPRSTDAGV*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pBabeD puro HA-TRAF6 KallR237.17 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65898
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 44060; Ser12 TCC>TCT to remove internal BamHI site silently; ; 23 Lysines mutated to Arginines:; K32, K67, K91, K96, K104, K124, K133, K142, K171, K180, K201, K252, K319, K331, K340, K356, K365, K371, K384, K388, K453, K469, K489
• Low Temp: No