cDNA Clone Sharpin

• Synonyms: SHANK associated RH domain interactor, SIPL1
• Plasmid: pGEX6P-1-Sharpin S165-L348 L179A/I183A/V271E
• Parent Plasmid: pGEX6P-1
• Expressed: GST-Sharpin S165-L348 L179A/I183A/V271E
• Genbank: NM_030974.4 → NP_112236.3
• Tag: GST
• Species: Human

Sequence of Insert

ggatccAGCCCTGGAAACTTGACGGAGAGAGAAGAGCTGGCAGGGAGCgcGGCCCGGGCTgcTGCAGGTGGAGACGAGAAGGGGGCAGCCCAAGTGGCAGCCGTCCTGGCCCAGCATCGTGTGGCCCTGAGTGTTCAGCTTCAGGAGGCCTGCTTCCCACCTGGCCCCATCAGGCTGCAGGTCACACTTGAAGACGCTGCCTCTGCCGCATCCGCCGCGTCCTCTGCACACGTTGCCCTGCAGGTCCACCCCCACTGCACTGTTGCAGCTCTCCAGGAGCAGGTGTTCTCAGAGCTCGGTTTCCCGCCAGCCGTGCAACGCTGGGagATCGGACGGTGCCTGTGTGTGCCTGAGCGCAGCCTTGCCTCTTACGGGGTTCGGCAGGATGGGGACCCTGCTTTCCTCTACTTGCTGTCAGCTCCcCGAGAAGCCCCAGCCACAGGACCTAGCCCTCAGCACCCCCAGAAGATGGACGGGGAACTTGGACGCTTGTTTCCCCCATCATTGGGGCTACCCCCAGGCCCCCAGCCAGCTGCCTCCAGCCTGCCCAGTCCACTCtaggcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSPGNLTEREELAGSAARAAAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASAASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWEIGRCLCVPERSLASYGVRQDGDPAFLYLLSAPREAPATGPSPQHPQKMDGELGRLFPPSLGLPPGPQPAASSLPSPL*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P-1-Sharpin S165-L348 L179A/I183A/V271E134.13 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65945
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-term GST tag on Sharpin's LTM-UBL domain with mutations in LTM and UBL; Contains silent change at Pro303 (CCT > CCC) to knock out native Xho1 site at nt909.; Transcript Variant 1
• Low Temp: No