cDNA Clone Sharpin

• Synonyms: SHANK-associated RH domain interactor, DKFZp434N1923, SIPL1, hSIPL1, sharpin, shank-associated RH domain-interacting protein, shank-interacting protein-like 1
• Plasmid: pGEX6P-1-Sharpin Transcript Variant 1
• Parent Plasmid: pGEX6P1
• Expressed: GST Sharpin
• Genbank: NM_030974.4 → NP_112236.3
• Tag: GST
• Species: Human

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAPPAGGAAAAASDLGSAAVLLAVHAAVRPLGAGPDAEAQLRRLQLSADPERPGRFRLELLGAGPGAVNLEWPLESVSYTIRGPTQHELQPPPGGPGTLSLHFLNPQEAQRWAVLVRGATVEGQNGSKSNSPPALGPEACPVSLPSPPEASTLKGPPPEADLPRSPGNLTEREELAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASAASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDPAFLYLLSAPREAPATGPSPQHPQKMDGELGRLFPPSLGLPPGPQPAASSLPSPLQPSWSCPSCTFINAPDRPGCEMCSTQRPCTWDPLAAAST*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P-1-Sharpin Transcript Variant 1328.92 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65992
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Full length Transcript Variant 1.; RT-PCR product comprising C-terminal 260bp section (comprising NZF domain missing from 32976) flanked Xho-Not1 (using native Xho1 site at nt 903) ligated to Xho-Not cut 32976 to build TV1; ; Silent change compared to NCBI ref at Arg52 CGG>AGG
• Low Temp: No