cDNA Clone TIFA

• Plasmid: pcDNA5D FRT TO (Ubc Pr)-TIFA-HA
• Parent Plasmid: pcDNA5D FRT TO (UbC Pr)
• Expressed: TIFA-HA
• Genbank: AB062110.1
• Tag: HA
• Species: Human

Sequence of Insert

cttaagATGACCAGTTTTGAAGATGCTGACACAGAAGAGACAGTAACTTGTCTCCAGATGACGGTTTACCATCCTGGCCAGTTGCAGTGTGGAATATTTCAGTCAATAAGTTTTAACAGAGAGAAACTCCCTTCCAGCGAAGTGGTGAAATTTGGCCGAAATTCCAACATCTGTCATTATACTTTTCAGGACAAACAGGTTTCCCGAGTTCAGTTTTCTCTGCAGCTGTTTAAAAAATTCAACAGCTCAGTTCTCTCCTTTGAAATAAAAAATATGAGTAAAAAGACCAATCTGATCGTGGACAGCAGAGAGCTGGGCTACCTAAATAAAATGGACCTGCCATACAGGTGCATGGTCAGATTCGGAGAGTATCAGTTTCTGATGGAGAAGGAAGATGGCGAGTCATTGGAATTTTTTGAGACTCAATTTATTTTATCTCCAAGATCACTCTTGCAAGAAAACAACTGGCCACCACACAGGCCCATACCGGAGTATGGCACTTACTCGCTCTGCTCCTCCCAAAGCAGTTCTCCGACAGAAATGGATGAAAATGAGTCATacccatacgatgtgccagattacgcctGAGCGGCCGC

Amino Acid Sequence

MTSFEDADTEETVTCLQMTVYHPGQLQCGIFQSISFNREKLPSSEVVKFGRNSNICHYTFQDKQVSRVQFSLQLFKKFNSSVLSFEIKNMSKKTNLIVDSRELGYLNKMDLPYRCMVRFGEYQFLMEKEDGESLEFFETQFILSPRSLLQENNWPPHRPIPEYGTYSLCSSQSSSPTEMDENESYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5D FRT TO (Ubc Pr)-TIFA-HA2.17 MB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU71044
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 70173 (pcDNA5D FRT TO Ubc Pr) 3HA tag removed by cloning TIFA-HA into the upstream AflII site.; No Kozak sequence
• Low Temp: No