cDNA Clone
p97 E305Q

Synonyms
CDC48/p97/VCP
Plasmid
pK27SUMO
Expressed
p97 E305Q (Human)
Genbank
NP_009057.1
Tag
SUMO
10His
Amino Acid Sequence
MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLK
GKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPID
DTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDT
VIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRG
ILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAI
IFIDqLDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRF
GRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAAL
QAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFI
SIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRV
INQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKAN
LRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAM
EVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG
Vector Type
Bacterial
Antibiotic
Kan
Full Snapgene Map
pK27SUMO151.15 KB Snapgene files can be opened using software available here: www.snapgene.com
DU Number
DU73312
Unit Source
Protein Phosphorylation and Ubiquitylation Unit
Location
PPU
MTA
Dundee
Notes
DU73312. Deposited by Karim Labib group.

14His-Smt3-human p97 E305Q (PCR mutagenesis from DU73310 by oligos 9198+9199)

Mutation in D1 walker B domain (Defective for ATP hydrolysis)
Low Temp
No
Price
£125.00
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