cDNA Clone RILPL1

• Plasmid: pET15b 6His sumo 12xRILPL1 391-403
• Parent Plasmid: pET15b 6His SUMO
• Expressed: 6HIS-SUMO-12x[GSGS-RILPL1(391-403)]
• Genbank: NM_178314.4
• Tag: 6His
• Tag: SUMO
• Species: Human

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGGSGSGYTEQGQEALQHLGSGSGYTEQGQEALQHLGSGSGYTEQGQEALQHLGSGSGYTEQGQEALQHLGSGSGYTEQGQEALQHLGSGSGYTEQGQ
EALQHLGSGSGYTEQGQEALQHLGSGSGYTEQGQEALQHLGSGSGYTEQGQEALQHLGSGSGYTEQGQEALQHLGSGSGYTEQGQEALQHLGSGSGYTEQ
GQEALQHL*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pET15b 6His sumo 12xRILPL1 391-403105.91 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU73939
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 12 repeats of RILPL1(391-403) with GSGS linker between the repeats, N-terminal His-SUMO with GSGS linker between the tag and the protein.; By recombination:; Amplified pET15 6His sumo: RT19216/19217: 6 kb; Amplified insert from synthesised seq: RT19218/19219 640 bp
• Low Temp: No