cDNA Clone S6K2

• Plasmid: pFBHTb p70S6K2 1-397
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-p70S6K2 1-397
• Genbank: NM_003952
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCGGCCGTGTTTGATTTGGATTTGGAGACGGAGGAAGGCAG
CGAGGGCGAGGGCGAGCCAGAGCTCAGCCCCGCGGACGCATGTCCCCTTG
CCGAGTTGAGGGCAGCTGGCCTAGAGCCTGTGGGACACTATGAAGAGGTG
GAGCTGACTGAGACCAGCGTGAACGTTGGCCCAGAGCGCATCGGGCCCCA
CTGCTTTGAGCTGCTGCGTGTGCTGGGCAAGGGGGGCTATGGCAAGGTGT
TCCAGGTGCGAAAGGTGCAAGGCACCAACTTGGGCAAAATATATGCCATG
AAAGTCCTAAGGAAGGCCAAAATTGTGCGCAATGCCAAGGACACAGCACA
CACACGGGCTGAGCGGAACATTCTAGAGTCAGTGAAGCACCCCTTTATTG
TGGAACTGGCCTATGCCTTCCAGACTGGTGGCAAACTCTACCTCATCCTT
GAGTGCCTCAGTGGTGGCGAGCTCTTCACGCATCTGGAGCGAGAGGGCAT
CTTCCTGGAAGATACGGCCTGCTTCTACCTGGCTGAGATCACGCTGGCCC
TGGGCCATCTCCACTCCCAGGGCATCATCTACCGGGACCTCAAGCCCGAG
AACATCATGCTCAGCAGCCAGGGCCACATCAAACTGACCGACTTTGGACT
CTGCAAGGAGTCTATCCATGAGGGCGCCGTCACTCACACCTTCTGCGGCA
CCATTGAGTACATGGCCCCTGAGATTCTGGTGCGCAGTGGCCACAACCGG
GCTGTGGACTGGTGGAGCCTGGGGGCCCTGATGTACGACATGCTCACTGG
ATCGCCGCCCTTCACCGCAGAGAACCGGAAGAAAACCATGGATAAGATCA
TCAGGGGCAAGCTGGCATTGCCCCCCTACCTCACCCCAGATGCCCGGGAC
CTTGTCAAAAAGTTTCTGAAACGGAATCCCAGCCAGCGGATTGGGGGTGG
CCCAGGGGATGCTGCTGATGTGCAGAGACATCCCTTTTTCCGGCACATGA
ATTGGGACGACCTTCTGGCCTGGCGTGTGGACCCCCCTTTCAGGCCCTGT
CTGCAGTCAGAGGAGGACGTGAGCCAGTTTGATACCCGCTTCACACGGCA
GACGCCGGTGGACAGTCCTGATGACACAGCCCTCAGCGAGAGTGCCAACC
AGGCCTTCCTGGGCTTCACATACGTGGCGCCGTCTGTCCTGGACAGCTAA
GCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMAAVFDLDLETEEGSEGEGEPE
LSPADACPLAELRAAGLEPVGHYEEVELTETSVNVGPERIGPHCFELLRV
LGKGGYGKVFQVRKVQGTNLGKIYAMKVLRKAKIVRNAKDTAHTRAERNI
LESVKHPFIVELAYAFQTGGKLYLILECLSGGELFTHLEREGIFLEDTAC
FYLAEITLALGHLHSQGIIYRDLKPENIMLSSQGHIKLTDFGLCKESIHE
GAVTHTFCGTIEYMAPEILVRSGHNRAVDWWSLGALMYDMLTGSPPFTAE
NRKKTMDKIIRGKLALPPYLTPDARDLVKKFLKRNPSQRIGGGPGDAADV
QRHPFFRHMNWDDLLAWRVDPPFRPCLQSEEDVSQFDTRFTRQTPVDSPD
DTALSESANQAFLGFTYVAPSVLDS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3843
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His for baculovirus expression.; Calc Mol Weight : 47546 Daltons