cDNA Clone PKA

• Synonyms: PRKACA, PKA C-alpha, PKACa
• Plasmid: pFBHTb PKA
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-PKA
• Genbank: P17612
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCGGCAACGCCGCCGCCGCCAAGAAGGGCAGCGAGCAGGAGAGCGT
GAAAGAATTCTTAGCCAAAGCCAAAGAAGATTTTCTTAAAAAATGGGAAA
GTCCCGCTCAGAACACAGCCCACTTGGATCAGTTTGAACGAATCAAGACC
CTCGGCACGGGCTCCTTCGGGCGGGTGATGCTGGTGAAACACAAGGAGAC
CGGGAACCACTATGCCATGAAGATCCTCGACAAACAGAAGGTGGTGAAAC
TGAAACAGATCGAACACACCCTGAATGAAAAGCGCATCCTGCAAGCTGTC
AACTTTCCGTTCCTCGTCAAACTCGAGTTCTCCTTCAAGGACAACTCAAA
CTTATACATGGTCATGGAGTACGTGCCCGGCGGGGAGATGTTCTCACACC
TACGGCGGATCGGAAGGTTCAGTGAGCCCCATGCCCGTTTCTACGCGGCC
CAGATCGTCCTGACCTTTGAGTATCTGCACTCGCTGGATCTCATCTACAG
GGACCTGAAGCCGGAGAATCTGCTCATTGACCAGCAGGGCTACATTCAGG
TGACAGACTTCGGTTTCGCCAAGCGCGTGAAGGGCCGCACTTGGACCTTG
TGCGGCACCCCTGAGTACCTGGCCCCTGAGATTATCCTGAGCAAAGGCTA
CAACAAGGCCGTGGACTGGTGGGCCCTGGGGGTTCTTATCTATGAAATGG
CCGCTGGCTACCCGCCCTTCTTCGCAGACCAGCCCATCCAGATCTATGAG
AAGATCGTCTCTGGGAAGGTGCGCTTCCCTTCCCACTTCAGCTCTGACTT
GAAGGACCTGCTGCGGAACCTCCTGCAGGTAGATCTCACCAAGCGCTTTG
GGAACCTCAAGAATGGGGTCAACGATATCAAGAACCACAAGTGGTTTGCC
ACAACTGACTGGATTGCCATCTACCAGAGGAAGGTGGAAGCTCCCTTCAT
ACCAAAGTTTAAAGGCCCTGGGGATACGAGTAACTTTGACGACTATGAGG
AAGAAGAAATCCGGGTCTCCATCAATGAGAAGTGTGGCAAGGAGTTTTCT
GAGTTTTAGGTCGAC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSGNAAAAMGNAAAAKKGSEQESV
KEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKET
GNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSN
LYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYR
DLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGY
NKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDL
KDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFI
PKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3680
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6HIS fusion for baculovirus expression.