cDNA Clone VPS15

• Plasmid: pFBHTc FLAG VPS15
• Parent Plasmid: pFastbac HTc
• Expressed: 6His-FLAG VPS15
• Genbank: NP_055417
• Tag: 6His
• Tag: FLAG
• Species: Human

Sequence of Insert

GTCGACATGGACTACAAGGACGATGACGATAAGGGAAATCAGCTTGCTGG
CATTGCTCCCTCCCAGATCCTTTCTGTAGAGAGTTATTTTTCAGATATTC
ATGACTTTGAATATGATAAAAGCCTGGGGAGTACTCGGTTTTTTAAAGTT
GCTCGAGCCAAGCACCGAGAAGGCCTGGTCGTTGTGAAGGTTTTTGCAAT
TCAGGATCCCACATTGCCTTTAACCAGCTATAAACAAGAGCTGGAGGAAC
TGAAAATCAGGCTTAATTCTGCACAGAATTGTCTACCTTTCCAGAAAGCA
TCAGAAAAAGCATCTGAGAAAGCAGCTATGCTCTTTAGGCAGTATGTGCG
AGACAATCTCTATGATCGCATCAGTACCCGTCCATTCTTGAATAACATTG
AGAAGCGCTGGATTGCTTTCCAGATCCTGACAGCTGTGGACCAAGCACAC
AAATCTGGAGTTCGTCATGGGGACATCAAGACTGAGAATGTGATGGTCAC
CAGTTGGAATTGGGTTCTTCTAACTGATTTTGCCAGTTTTAAGCCCACTT
ATCTTCCAGAAGACAACCCGGCAGATTTCAATTATTTCTTTGACACATCA
CGGAGGAGAACTTGCTATATTGCTCCTGAACGTTTTGTTGATGGTGGGAT
GTTTGCCACTGAGTTAGAATATATGAGAGATCCTTCAACTCCGCTTGTAG
ACTTAAATAGCAATCAGAGAACAAGAGGAGAGTTGAAGAGAGCAATGGAC
ATCTTTTCAGCAGGTTGTGTGATAGCTGAGCTTTTTACAGAAGGTGTACC
ATTATTTGATCTCTCTCAACTTTTGGCTTATAGAAATGGACATTTTTTCC
CTGAACAAGTGCTAAATAAAATTGAAGATCACAGTATCAGAGAATTGGTA
ACTCAGATGATTCACCGTGAGCCAGATAAACGTTTAGAGGCAGAAGATTA
CTTAAAACAGCAGCGTGGCAATGCCTTTCCTGAAATATTTTACACTTTTC
TTCAGCCCTACATGGCCCAGTTTGCCAAGGAAACGTTTCTTTCTGCAGAT
GAGCGTATTCTGGTTATACGGAAGGATTTGGGCAACATTATTCACAATCT
CTGTGGACATGATCTGCCAGAAAAAGCCGAAGGAGAGCCTAAGGAAAATG
GGCTGGTTATCTTGGTATCTGTTATAACATCCTGCCTACAGACCCTTAAA
TACTGTGATTCCAAACTAGCTGCTTTGGAACTGATTCTTCATTTGGCTCC
AAGATTAAGTGTTGAAATCCTTTTGGATCGTATTACTCCATATCTTTTGC
ATTTCAGCAATGACTCTGTTCCTAGGGTGAGGGCTGAAGCCTTGAGGACG
TTGACCAAAGTTCTTGCTCTCGTCAAAGAGGTTCCTCGTAATGATATCAA
TATTTATCCGGAATACATTCTGCCAGGCATAGCCCACTTAGCCCAAGATG
ATGCTACTATCGTTAGACTAGCCTATGCTGAAAACATAGCTCTGCTGGCA
GAAACAGCTCTGAGATTCCTGGAATTAGTACAGTTAAAAAATCTTAATAT
GGAAAATGACCCCAATAATGAAGAAATAGATGAGGTTACACATCCAAATG
GAAATTATGACACAGAGCTCCAAGCCTTACATGAAATGGTCCAGCAGAAA
GTTGTTACTTTGCTAAGTGACCCTGAAAATATTGTAAAACAAACCTTGAT
GGAAAATGGAATAACACGGCTGTGTGTATTCTTTGGACGTCAGAAAGCCA
ACGATGTTTTGTTGTCCCACATGATTACTTTCCTAAATGATAAGAATGAT
TGGCATCTACGTGGAGCATTTTTTGATAGTATAGTTGGTGTTGCTGCCTA
TGTTGGCTGGCAAAGCTCCTCAATTCTCAAGCCTCTGCTGCAACAAGGTC
TTAGTGATGCTGAGGAATTTGTCATTGTGAAAGCTCTTTATGCCCTTACT
TGTATGTGCCAGTTAGGACTGCTACAAAAACCCCATGTTTACGAATTTGC
CAGTGATATTGCCCCCTTCCTGTGTCATCCCAATTTATGGATACGTTATG
GTGCCGTGGGATTTATCACAGTGGTAGCTCGTCAAATAAGTACAGCTGAT
GTCTACTGTAAACTGATGCCTTATCTTGACCCATATATTACCCAACCAAT
AATACAGATTGAAAGAAAACTTGTTCTGCTCAGTGTTTTAAAGGAACCAG
TAAGTCGTTCTATATTTGATTATGCTTTGAGGTCTAAAGATATTACTAGC
TTGTTCAGACATCTTCACATGCGTCAGAAGAAACGAAATGGTTCTCTTCC
CGACTGCCCTCCGCCAGAGGATCCTGCCATAGCACAGCTTCTGAAGAAGT
TGCTCTCACAGGGAATGACAGAGGAAGAGGAAGACAAACTTCTGGCACTG
AAAGACTTCATGATGAAATCTAATAAAGCAAAGGCCAATATAGTGGACCA
GAGCCATCTTCATGATAGTAGTCAGAAAGGTGTAATTGACTTGGCAGCTT
TAGGCATAACTGGGAGACAAGTTGATCTTGTTAAAACCAAACAAGAACCA
GATGACAAACGGGCCAGAAAACATGTAAAACAAGACTCAAATGTAAATGA
AGAATGGAAAAGCATGTTTGGGTCACTGGACCCACCAAACATGCCACAGG
CCCTACCTAAAGGGAGTGATCAGGAGGTGATTCAGACTGGGAAACCTCCT
CGTTCCGAGTCCTCTGCTGGCATTTGTGTCCCTTTGTCAACTTCTTCACA
GGTTCCAGAAGTGACAACTGTCCAAAATAAAAAACCAGTAATACCGGTTT
TAAGTAGTACAATCTTACCATCCACCTATCAGATTCGAATTACAACTTGT
AAAACTGAACTTCAGCAACTCATCCAGCAAAAGCGGGAGCAGTGCAATGC
TGAGAGAATAGCTAAGCAGATGATGGAAAATGCTGAATGGGAGAGTAAAC
CACCACCACCTGGATGGCGTCCTAAAGGGCTGTTAGTTGCCCATCTTCAT
GAGCATAAATCTGCTGTGAATCGAATTAGAGTCTCTGATGAACACTCACT
TTTTGCAACATGTTCAAATGATGGCACAGTGAAAATCTGGAACAGTCAAA
AGATGGAGGGGAAGACCACCACTACCAGATCTATTCTTACATACAGCCGA
ATTGGAGGACGAGTCAAGACGCTCACATTCTGCCAAGGCTCCCACTATTT
AGCCATAGCATCTGATAATGGTGCTGTCCAGCTTCTTGGAATTGAGGCTT
CTAAGCTGCCCAAGTCTCCTAAAATCCATCCTCTACAAAGCAGAATTCTA
GATCAGAAGGAGGACGGTTGTGTTGTGGATATGCATCACTTCAACTCTGG
AGCACAGTCTGTTCTTGCCTATGCCACTGTGAATGGCTCTCTGGTTGGCT
GGGACCTTAGGTCTTCAAGCAATGCGTGGACTTTAAAGCATGATTTAAAG
TCGGGCCTCATCACTTCCTTTGCTGTGGACATCCACCAATGCTGGCTCTG
CATTGGTACAAGCAGTGGTACCATGGCTTGTTGGGACATGAGGTTCCAGT
TGCCAATTTCAAGTCACTGTCATCCTTCCAGGGCTCGAATCAGACGCCTC
TCAATGCACCCTCTGTATCAGTCCTGGGTGATTGCAGCTGTTCAGGGCAA
CAACGAAGTGTCCATGTGGGACATGGAGACTGGTGACAGAAGATTTACTC
TCTGGGCCAGCAGTGCACCACCACTTTCTGAATTACAGCCTTCTCCTCAT
AGCGTCCATGGTATCTACTGTAGTCCTGCAGATGGAAATCCTATCCTACT
AACAGCTGGCTCAGATATGAAAATAAGGTTTTGGGACTTGGCTTACCCAG
AAAGGTCCTATGTTGTTGCAGGAAGTACTAGTTCCCCATCTGTGTCCTAC
TACAGGAAAATAATTGAAGGCACTGAAGTTGTCCAGGAAATTCAGAATAA
GCAGAAAGTAGGACCAAGTGATGACACCCCTCGAAGGGGCCCAGAGTCCC
TGCCCGTGGGACATCATGACATCATCACTGATGTCGCCACATTCCAGACC
ACACAGGGCTTCATCGTAACTGCTTCTAGAGATGGGATTGTGAAGGTGTG
GAAATAAGTCGAC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGIRNSKAYVDMDYKDDDDKGNQLA
GIAPSQILSVESYFSDIHDFEYDKSLGSTRFFKVARAKHREGLVVVKVFA
IQDPTLPLTSYKQELEELKIRLNSAQNCLPFQKASEKASEKAAMLFRQYV
RDNLYDRISTRPFLNNIEKRWIAFQILTAVDQAHKSGVRHGDIKTENVMV
TSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERFVDGG
MFATELEYMRDPSTPLVDLNSNQRTRGELKRAMDIFSAGCVIAELFTEGV
PLFDLSQLLAYRNGHFFPEQVLNKIEDHSIRELVTQMIHREPDKRLEAED
YLKQQRGNAFPEIFYTFLQPYMAQFAKETFLSADERILVIRKDLGNIIHN
LCGHDLPEKAEGEPKENGLVILVSVITSCLQTLKYCDSKLAALELILHLA
PRLSVEILLDRITPYLLHFSNDSVPRVRAEALRTLTKVLALVKEVPRNDI
NIYPEYILPGIAHLAQDDATIVRLAYAENIALLAETALRFLELVQLKNLN
MENDPNNEEIDEVTHPNGNYDTELQALHEMVQQKVVTLLSDPENIVKQTL
MENGITRLCVFFGRQKANDVLLSHMITFLNDKNDWHLRGAFFDSIVGVAA
YVGWQSSSILKPLLQQGLSDAEEFVIVKALYALTCMCQLGLLQKPHVYEF
ASDIAPFLCHPNLWIRYGAVGFITVVARQISTADVYCKLMPYLDPYITQP
IIQIERKLVLLSVLKEPVSRSIFDYALRSKDITSLFRHLHMRQKKRNGSL
PDCPPPEDPAIAQLLKKLLSQGMTEEEEDKLLALKDFMMKSNKAKANIVD
QSHLHDSSQKGVIDLAALGITGRQVDLVKTKQEPDDKRARKHVKQDSNVN
EEWKSMFGSLDPPNMPQALPKGSDQEVIQTGKPPRSESSAGICVPLSTSS
QVPEVTTVQNKKPVIPVLSSTILPSTYQIRITTCKTELQQLIQQKREQCN
AERIAKQMMENAEWESKPPPPGWRPKGLLVAHLHEHKSAVNRIRVSDEHS
LFATCSNDGTVKIWNSQKMEGKTTTTRSILTYSRIGGRVKTLTFCQGSHY
LAIASDNGAVQLLGIEASKLPKSPKIHPLQSRILDQKEDGCVVDMHHFNS
GAQSVLAYATVNGSLVGWDLRSSSNAWTLKHDLKSGLITSFAVDIHQCWL
CIGTSSGTMACWDMRFQLPISSHCHPSRARIRRLSMHPLYQSWVIAAVQG
NNEVSMWDMETGDRRFTLWASSAPPLSELQPSPHSVHGIYCSPADGNPIL
LTAGSDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQN
KQKVGPSDDTPRRGPESLPVGHHDIITDVATFQTTQGFIVTASRDGIVKV
WK*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3587
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6His/FLAG tag for baculovirus expression.