cDNA Clone PHK liver

• Plasmid: pGEX4T-1-PBK liver delta 302-407
• Parent Plasmid: pGEX4T1
• Expressed: GST-PBK liver delta 302-407
• Genbank: M31606
• Tag: GST
• Species: Human

Sequence of Insert

GAATTCACGCTGGACGTGGGGCCGGAGGATGAGCTGCCCGACTGGGCCGC
CGCCAAAGAGTTTTACCAGAAGTACGACCCTAAGGACGTCATCGGCAGAG
GAGTGAGCTCTGTGGTCCGCCGTTGTGTTCATCGAGCTACTGGCCACGAG
TTTGCGGTGAAGATTATGGAAGTGACAGCTGAGCGGCTGAGTCCTGAGCA
GCTGGAGGAGGTGCGGGAAGCCACACGGCGAGAGACACACATCCTTCGCC
AGGTCGCCGGCCACCCCCACATCATCACCCTCATCGATTCCTACGAGTCT
TCTAGCTTCATGTTCCTGGTGTTTGACCTGATGCGGAAGGGAGAGCTGTT
TGACTATCTCACAGAGAAGGTGGCCCTCTCTGAAAAGGAAACCAGGTCCA
TCATGCGGTCTCTGCTGGAAGCAGTGAGCTTTCTCCATGCCAACAACATT
GTGCATCGAGATCTGAAGCCCGAGAATATTCTCCTAGATGACAATATGCA
GATCCGACTTTCAGATTTCGGGTTCTCCTGCCACTTGGAACCTGGCGAGA
AGCTTCGAGAGTTGTGTGGGACCCCAGGGTATCTAGCGCCAGAGATCCTT
AAATGCTCCATGGATGAAACCCACCCAGGCTATGGCAAGGAGGTCGACCT
CTGGGCCTGTGGGGTGATCTTGTTCACACTCCTGGCTGGCTCGCCACCCT
TCTGGCACCGGCGGCAGATCCTGATGTTACGCATGATCATGGAGGGCCAG
TACCAGTTCAGTTCCCCCGAGTGGGATGACCGTTCCAGCACTGTCAAAGA
CCTGATCTCCAGGCTGCTGCAGGTGGATCCTGAGGCACGCCTGACAGCTG
AGCAGGCCCTACAGCACCCCTTCTTTGAGCGTTGTGAAGGCAGCCAACCC
TGGAACTAACTCGAG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSPEFTLDVGPEDELPDWAAAKEFYQ
KYDPKDVIGRGVSSVVRRCVHRATGHEFAVKIMEVTAERLSPEQLEEVRE
ATRRETHILRQVAGHPHIITLIDSYESSSFMFLVFDLMRKGELFDYLTEK
VALSEKETRSIMRSLLEAVSFLHANNIVHRDLKPENILLDDNMQIRLSDF
GFSCHLEPGEKLRELCGTPGYLAPEILKCSMDETHPGYGKEVDLWACGVI
LFTLLAGSPPFWHRRQILMLRMIMEGQYQFSSPEWDDRSSTVKDLISRLL
QVDPEARLTAEQALQHPFFERCEGSQPWN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3530
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.