cDNA Clone TEV Protease

• Plasmid: pGEX6P-1-TEV protease 6His S218N
• Parent Plasmid: pGEX6P1
• Genbank: NP_734212
• Tag: 6His
• Species: Tobacco Etch Virus

Sequence of Insert

GGATCCGAAAGCTTGTTTAAGGGACCACGTGATTACAACCCGATATCGAG
CACCATTTGTCATTTGACGAATGAATCTGATGGGCACACAACATCGTTGT
ATGGTATTGGATTTGGTCCCTTCATCATTACAAACAAGCACTTGTTTAGA
AGAAATAATGGAACACTGTTGGTCCAATCACTACATGGTGTATTCAAGGT
CAAGAACACCACGACTTTGCAACAACACCTCATTGATGGGAGGGACATGA
TAATTATTCGCATGCCTAAGGATTTCCCACCATTTCCTCAAAAGCTGAAA
TTTAGAGAGCCACAAAGGGAAGAGCGCATATGTCTTGTGACAACCAACTT
CCAAACTAAGAGCATGTCTAGCATGGTGTCAGACACTAGTTGCACATTCC
CTTCATCTGATGGCATATTCTGGAAGCATTGGATTCAAACCAAGGATGGG
CAGTGTGGCAGTCCATTAGTATCAACTAGAGATGGGTTCATTGTTGGTAT
ACACTCAGCATCGAATTTCACCAACACAAACAATTATTTCACAAGCGTGC
CGAAAAACTTCATGGAATTGTTGACAAATCAGGAGGCGCAGCAGTGGGTT
AGTGGTTGGCGATTAAATGCTGACTCAGTATTGTGGGGGGGCCATAAAGT
TTTCATGAACAAACCTGAAGAGCCTTTTCAGCCAGTTAAGGAAGCGACTC
AACTCATGAATGAATTGGTGTACTCGCAACATCATCACCATCACCATTAA
GAATTCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSESLFKGPRDYNPISSTICH
LTNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLLVQSLHGVFKVKNTT
TLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKS
MSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSAS
NFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMNK
PEEPFQPVKEATQLMNELVYSQHHHHHH

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3522
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminal GST and C terminal 6His tags. S218N prevents autocleavage.