cDNA Clone PLK1

• Plasmid: pFB PLK1
• Parent Plasmid: pFastbac1 mod
• Expressed: 6His-PLK1
• Genbank: NM_005030
• Tag: 6His
• Species: Human

Sequence of Insert

CATATGAGTGCTGCAGTGACTGCAGGGAAGCTGGCACGGGCACCGGCCGA
CCCTGGGAAAGCCGGGGTCCCCGGAGTTGCAGCTCCCGGAGCTCCGGCGG
CGGCTCCACCGGCGAAAGAGATCCCGGAGGTCCTAGTGGACCCACGCAGC
CGGCGGCGCTATGTGCGGGGCCGCTTTTTGGGCAAGGGCGGCTTTGCCAA
GTGCTTCGAGATCTCGGACGCGGACACCAAGGAGGTGTTCGCGGGCAAGA
TTGTGCCTAAGTCTCTGCTGCTCAAGCCGCACCAGAGGGAGAAGATGTCC
ATGGAAATATCCATTCACCGCAGCCTCGCCCACCAGCACGTCGTAGGATT
CCACGGCTTTTTCGAGGACAACGACTTCGTGTTCGTGGTGTTGGAGCTCT
GCCGCCGGAGGTCTCTCCTGGAGCTGCACAAGAGGAGGAAAGCCCTGACT
GAGCCTGAGGCCCGATACTACCTACGGCAAATTGTGCTTGGCTGCCAGTA
CCTGCACCGAAACCGAGTTATTCATCGAGACCTCAAGCTGGGCAACCTTT
TCCTGAATGAAGATCTGGAGGTGAAAATAGGGGATTTTGGACTGGCAACC
AAAGTCGAATATGACGGGGAGAGGAAGAAGACCCTGTGTGGGACTCCTAA
TTACATAGCTCCCGAGGTGCTGAGCAAGAAAGGGCACAGTTTCGAGGTGG
ATGTGTGGTCCATTGGGTGTATCATGTATACCTTGTTAGTGGGCAAACCA
CCTTTTGAGACTTCTTGCCTAAAAGAGACCTACCTCCGGATCAAGAAGAA
TGAATACAGTATTCCCAAGCACATCAACCCCGTGGCCGCCTCCCTCATCC
AGAAGATGCTTCAGACAGATCCCACTGCCCGCCCAACCATTAACGAGCTG
CTTAATGACGAGTTCTTTACTTCTGGCTATATCCCTGCCCGTCTCCCCAT
CACCTGCCTGACCATTCCACCAAGGTTTTCGATTGCTCCCAGCAGCCTGG
ACCCCAGCAACCGGAAGCCCCTCACAGTCCTCAATAAAGGCTTGGAGAAC
CCCCTGCCTGAGCGTCCCCGGGAAAAAGAAGAACCAGTGGTTCGAGAGAC
AGGTGAGGTGGTCGACTGCCACCTCAGTGACATGCTGCAGCAGCTGCACA
GTGTCAATGCCTCCAAGCCCTCGGAGCGTGGGCTGGTCAGGCAAGAGGAG
GCTGAGGATCCTGCCTGCATCCCCATCTTCTGGGTCAGCAAGTGGGTGGA
CTATTCGGACAAGTACGGCCTTGGGTATCAGCTCTGTGATAACAGCGTGG
GGGTGCTCTTCAATGACTCAACACGCCTCATCCTCTACAATGATGGTGAC
AGCCTGCAGTACATAGAGCGTGACGGCACTGAGTCCTACCTCACCGTGAG
TTCCCATCCCAACTCCTTGATGAAGAAGATCACCCTCCTTAAATATTTCC
GCAATTACATGAGCGAGCACTTGCTGAAGGCAGGTGCCAACATCACGCCG
CGCGAAGGTGATGAGCTCGCCCGGCTGCCCTACCTACGGACCTGGTTCCG
CACCCGCAGCGCCATCATCCTGCACCTCAGCAACGGCAGCGTGCAGATCA
ACTTCTTCCAGGATCACACCAAGCTCATCTTGTGCCCACTGATGGCAGCC
GTGACCTACATCGACGAGAAGCGGGACTTCCGCACATACCGCCTGAGTCT
CCTGGAGGAGTACGGCTGCTGCAAGGAGCTGGCCAGCCGGCTCCGCTACG
CCCGCACTATGGTGGACAAGCTGCTGAGCTCACGCTCGGCCAGCAACCGT
CTCAAGGCCTCCTAACTCGAG

Amino Acid Sequence

MHHHHHHMSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEV
LVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPH
QREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHK
RRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIG
DFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYT
LLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTAR
PTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVL
NKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERG
LVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLI
LYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKA
GANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLIL
CPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSS
RSASNRLKAS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU3482
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal 6his tag for baculovirus expression.