cDNA Clone 14-3-3 zeta

• Synonyms: YWHAZ
• Plasmid: ECFP-C1 14-3-3-zeta
• Parent Plasmid: pECFPC1
• Expressed: CFP-14-3-3-zeta
• Genbank: NM_145690
• Tag: CFP
• Species: Human

Sequence of Insert

GGATCCGATAAAAATGAGCTGGTTCAGAAGGCCAAACTGGCCGAGCAGGC
TGAGCGATATGATGACATGGCAGCCTGCATGAAGTCTGTAACTGAGCAAG
GAGCTGAATTATCCAATGAGGAGAGGAATCTTCTCTCAGTTGCTTATAAA
AATGTTGTAGGAGCCCGTAGGTCATCTTGGAGGGTCGTCTCAAGTATTGA
ACAAAAGACGGAAGGTGCTGAGAAAAAACAGCAGATGGCTCGAGAATACA
GAGAGAAAATTGAGACGGAGCTAAGAGATATCTGCAATGATGTACTGTCT
CTTTTGGAAAAGTTCTTGATCCCCAATGCTTCACAAGCAGAGAGCAAAGT
CTTCTATTTGAAAATGAAAGGAGATTACTACCGTTACTTGGCTGAGGTTG
CCGCTGGTGATGACAAGAAAGGGATTGTCGATCAGTCACAACAAGCATAC
CAAGAAGCTTTTGAAATCAGCAAAAAGGAAATGCAACCAACACATCCTAT
CAGACTGGGTCTGGCCCTTAACTTCTCTGTGTTCTATTATGAGATTCTGA
ACTCCCCAGAGAAAGCCTGCTCTCTTGCAAAGACAGCTTTTGATGAAGCC
ATTGCTGAACTTGATACATTAAGTGAAGAGTCATACAAAGACAGCACGCT
AATAATGCAATTACTGAGAGACAACTTGACATTGTGGACATCGGATACCC
AAGGAGACGAAGCTGAAGCAGGAGAAGGAGGGGAAAATTAAGGATCC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHN
VYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEE
RNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETEL
RDICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKG
IVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACS
LAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAG
EGGEN*

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU3183
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal CFP for mammalian expression.