cDNA Clone 14-3-3 zeta

• Synonyms: YWHAZ
• Plasmid: pGEX6P-1-14-3-3-zeta
• Parent Plasmid: pGEX6P1
• Expressed: GST-14-3-3-zeta
• Genbank: NM_145690
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGATAAAAATGAGCTGGTTCAGAAGGCCAAACTGGCCGAGCAGGC
TGAGCGATATGATGACATGGCAGCCTGCATGAAGTCTGTAACTGAGCAAG
GAGCTGAATTATCCAATGAGGAGAGGAATCTTCTCTCAGTTGCTTATAAA
AATGTTGTAGGAGCCCGTAGGTCATCTTGGAGGGTCGTCTCAAGTATTGA
ACAAAAGACGGAAGGTGCTGAGAAAAAACAGCAGATGGCTCGAGAATACA
GAGAGAAAATTGAGACGGAGCTAAGAGATATCTGCAATGATGTACTGTCT
CTTTTGGAAAAGTTCTTGATCCCCAATGCTTCACAAGCAGAGAGCAAAGT
CTTCTATTTGAAAATGAAAGGAGATTACTACCGTTACTTGGCTGAGGTTG
CCGCTGGTGATGACAAGAAAGGGATTGTCGATCAGTCACAACAAGCATAC
CAAGAAGCTTTTGAAATCAGCAAAAAGGAAATGCAACCAACACATCCTAT
CAGACTGGGTCTGGCCCTTAACTTCTCTGTGTTCTATTATGAGATTCTGA
ACTCCCCAGAGAAAGCCTGCTCTCTTGCAAAGACAGCTTTTGATGAAGCC
ATTGCTGAACTTGATACATTAAGTGAAGAGTCATACAAAGACAGCACGCT
AATAATGCAATTACTGAGAGACAACTTGACATTGTGGACATCGGATACCC
AAGGAGACGAAGCTGAAGCAGGAGAAGGAGGGGAAAATTAAGGATCC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSDKNELVQKAKLAEQAERYD
DMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTE
GAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKVFYLK
MKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGL
ALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQL
LRDNLTLWTSDTQGDEAEAGEGGEN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU3174
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N terminal GST for bacterial expression.